PMID- 38096640
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240214
IS  - 1878-0407 (Electronic)
IS  - 1744-117X (Linking)
VI  - 49
DP  - 2024 Mar
TI  - Transcriptome analysis reveals immune function-related mRNA expression in donkey 
      mammary glands during four developmental stages.
PG  - 101169
LID - S1744-117X(23)00114-4 [pii]
LID - 10.1016/j.cbd.2023.101169 [doi]
AB  - The low susceptibility to mastitis of female donkey (jenny) mammary glands and 
      the strong immune properties of donkey milk are acknowledged, but little is known 
      about the genes involved in mammary gland immunity in jennies. Herein, we used 
      RNA-sequencing and bioinformatics analyses to explore jenny mammary gland 
      transcriptomes and detect potential functional differentially expressed (DE) 
      mRNAs related to immunity during four specific developmental stages: foetal (F), 
      pubertal (P), adult parous nonlactation (N) and lactation (L). A total of 2497, 
      583 and 1820 DE mRNAs were identified in jenny mammary glands at F vs. P, P vs. 
      N, and N vs. L, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Gene 
      and Genomes (KEGG) analyses revealed numerous GO terms related to immune 
      function, especially between F and P. Seven significantly enriched profiles were 
      identified, among which 497 and 1261 DE mRNAs were upregulated in profiles 19 and 
      17. Eleven mRNAs were enriched in over 10 KEGG pathways. beta-2-microglobulin (B2M), 
      immunoglobulin heavy constant mu (IGHM), toll like receptor 2 (TLR2), toll like 
      receptor 4 (TLR4) and myeloid differentiation factor 88 (MYD88) were mainly 
      involved in phosphoinositide 3-kinase (PI3K)-Akt signalling, phagosome and 
      nuclear factor kappa-B (NF-kappa B) signalling pathways. The findings provide 
      insight into the molecular features underpinning the low prevalence of 
      intramammary infections (i.e., mastitis) in donkeys.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Wang, Xinyue
AU  - Wang X
AD  - Department of Animal Genetics, Breeding and Reproduction, College of Animal 
      Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 
      110866, China.
FAU - Fei, Yaqi
AU  - Fei Y
AD  - Department of Animal Genetics, Breeding and Reproduction, College of Animal 
      Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 
      110866, China.
FAU - Shao, Yang
AU  - Shao Y
AD  - Department of Animal Genetics, Breeding and Reproduction, College of Animal 
      Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 
      110866, China.
FAU - Liao, Qingchao
AU  - Liao Q
AD  - Department of Animal Genetics, Breeding and Reproduction, College of Animal 
      Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 
      110866, China.
FAU - Meng, Qingze
AU  - Meng Q
AD  - Department of Animal Genetics, Breeding and Reproduction, College of Animal 
      Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 
      110866, China.
FAU - Chen, Ran
AU  - Chen R
AD  - Department of Animal Genetics, Breeding and Reproduction, College of Animal 
      Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 
      110866, China.
FAU - Deng, Liang
AU  - Deng L
AD  - Department of Animal Genetics, Breeding and Reproduction, College of Animal 
      Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 
      110866, China. Electronic address: ldeng@syau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20231207
PL  - Netherlands
TA  - Comp Biochem Physiol Part D Genomics Proteomics
JT  - Comparative biochemistry and physiology. Part D, Genomics & proteomics
JID - 101270611
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Female
MH  - Animals
MH  - Humans
MH  - *Equidae/genetics/metabolism
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - Gene Expression Profiling
MH  - Transcriptome
MH  - RNA, Messenger/genetics
MH  - *Mastitis
MH  - Immunity
OTO - NOTNLM
OT  - Developmental stage
OT  - Donkey mammary gland
OT  - Immune function
OT  - Intramammary infection
OT  - Transcriptomics
COIS- Declaration of competing interest The authors have no conflicts of interests to 
      declare.
EDAT- 2023/12/15 00:43
MHDA- 2024/02/13 12:46
CRDT- 2023/12/14 18:02
PHST- 2023/08/13 00:00 [received]
PHST- 2023/11/29 00:00 [revised]
PHST- 2023/11/30 00:00 [accepted]
PHST- 2024/02/13 12:46 [medline]
PHST- 2023/12/15 00:43 [pubmed]
PHST- 2023/12/14 18:02 [entrez]
AID - S1744-117X(23)00114-4 [pii]
AID - 10.1016/j.cbd.2023.101169 [doi]
PST - ppublish
SO  - Comp Biochem Physiol Part D Genomics Proteomics. 2024 Mar;49:101169. doi: 
      10.1016/j.cbd.2023.101169. Epub 2023 Dec 7.