PMID- 38097757
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20231227
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Dec 14
TI  - Soil microbial improvement using enriched vinasse as a new abundant waste.
PG  - 22279
LID - 10.1038/s41598-023-49401-w [doi]
LID - 22279
AB  - This study proposes the use of vinasse, an inexpensive and readily available 
      waste biopolymer, as a fundamental component of a waste culture medium that can 
      enhance the effectiveness and cost-efficiency of the microbial-induced calcite 
      precipitation (MICP) method for sustainable soil improvement. Vinasse enriched 
      with urea, sodium caseinate, or whey protein concentrate is employed to optimize 
      bacterial growth and urease activity of Sporosarcina pasteurii (S. pasteurii) 
      bacterium. The best culture medium is analyzed using Taguchi design of 
      experiments (TDOE) and statistical analysis, considering the concentration of 
      vinasse and urea as effective parameters during growth time. To test the best 
      culture medium for bio-treated soil, direct shear tests were performed on loose 
      and bio-treated sand. The results demonstrate a substantial cost reduction from 
      $0.455 to $0.005 per liter when using the new culture medium (vinasse and urea) 
      compared to the conventional Nutrient Broth (NB) culture medium. Additionally, 
      the new medium enhances soil shear strength, increasing the friction angle by 2.5 
      degrees and cohesion to 20.7 kPa compared to the conventional medium. 
      Furthermore, the recycling of vinasse as a waste product can promote the progress 
      of a circular economy and reduce environmental pollution. As ground improvement 
      is essential for many construction projects, especially those that require high 
      shear strength or are built on loose soil, this study provides a promising 
      approach to achieving cost-effective and sustainable soil microbial improvement 
      using enriched vinasse.
CI  - (c) 2023. The Author(s).
FAU - Kariminia, Tahereh
AU  - Kariminia T
AD  - Department of Civil Engineering, Isfahan University of Technology, Isfahan, 
      84156-83111, Iran.
FAU - Rowshanzamir, Mohammad A
AU  - Rowshanzamir MA
AD  - Department of Civil Engineering, Isfahan University of Technology, Isfahan, 
      84156-83111, Iran.
FAU - Abtahi, S Mahdi
AU  - Abtahi SM
AD  - Department of Civil Engineering, Isfahan University of Technology, Isfahan, 
      84156-83111, Iran. mabtahi@iut.ac.ir.
FAU - Soleimanian-Zad, Sabihe
AU  - Soleimanian-Zad S
AD  - Department of Food Science and Technology, College of Agriculture, Isfahan 
      University of Technology, Isfahan, 84156-83111, Iran.
AD  - Research Institute for Biotechnology and Bioengineering, Isfahan University of 
      Technology, Isfahan, 84156-83111, Iran.
FAU - Bak, Hamid Mortazavi
AU  - Bak HM
AD  - School of Civil and Environmental Engineering, University of New South Wales, 
      Sydney, 2052, Australia.
FAU - Baghbanan, Alireza
AU  - Baghbanan A
AD  - Department of Mining Engineering, Isfahan University of Technology, Isfahan, 
      84156-83111, Iran.
LA  - eng
PT  - Journal Article
DEP - 20231214
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Soil)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - 8W8T17847W (Urea)
RN  - 0 (Culture Media)
SB  - IM
MH  - *Soil
MH  - *Soil Microbiology
MH  - Calcium Carbonate/metabolism
MH  - Bacteria/metabolism
MH  - Urea/metabolism
MH  - Culture Media/pharmacology
PMC - PMC10721901
COIS- The authors declare no competing interests.
EDAT- 2023/12/15 00:43
MHDA- 2023/12/17 17:43
PMCR- 2023/12/14
CRDT- 2023/12/14 23:47
PHST- 2023/06/11 00:00 [received]
PHST- 2023/12/07 00:00 [accepted]
PHST- 2023/12/17 17:43 [medline]
PHST- 2023/12/15 00:43 [pubmed]
PHST- 2023/12/14 23:47 [entrez]
PHST- 2023/12/14 00:00 [pmc-release]
AID - 10.1038/s41598-023-49401-w [pii]
AID - 49401 [pii]
AID - 10.1038/s41598-023-49401-w [doi]
PST - epublish
SO  - Sci Rep. 2023 Dec 14;13(1):22279. doi: 10.1038/s41598-023-49401-w.