PMID- 38099985
OWN - NLM
STAT- MEDLINE
DCOM- 20240311
LR  - 20240311
IS  - 1573-6857 (Electronic)
IS  - 0016-6707 (Linking)
VI  - 152
IP  - 1
DP  - 2024 Feb
TI  - Insulin and circadian rhythm genes of the Nile rat (Arvicanthis niloticus) are 
      conserved and orthologous to those in the rat, mouse and human.
PG  - 11-29
LID - 10.1007/s10709-023-00202-z [doi]
AB  - The African grass or Nile rat (NR) (Arvicanthis niloticus) is a herbivorous 
      diurnal rodent which is used as a biological model for research on type 2 
      diabetes mellitus (T2DM) and the circadian rhythm. Similar to humans, male NRs 
      develop T2DM with high-carbohydrate diets. The NR thus provides a unique 
      opportunity to identify the nutritional and underlying genetic factors that 
      characterise human T2DM, as well as the effects of potential anti-diabetic 
      phytochemicals such as Water-Soluble Palm Fruit Extract. Whole genome sequencing 
      (WGS) could help identify possible genetic causes why NRs spontaneously develop 
      T2DM in captivity. In this study, we performed WGS on a hepatic deoxyribonucleic 
      acid (DNA) sample isolated from a male NR using PacBio high-fidelity long-read 
      sequencing. The WGS data obtained were then de novo assembled and annotated using 
      PacBio HiFi isoform sequencing (Iso-Seq) data as well as previous Illumina RNA 
      sequencing (RNA-Seq) data. Genes related to insulin and circadian rhythm pathways 
      were present in the NR genome, similar to orthologues in the rat, mouse and human 
      genomes. T2DM development in the NR is thus most likely not attributable to 
      structural differences in these genes when compared to other biological models. 
      Further studies are warranted to gain additional insights on the 
      genetic-environmental factors which underlie the genetic permissiveness of NRs to 
      develop T2DM.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.
FAU - Leow, Soon-Sen
AU  - Leow SS
AD  - Malaysian Palm Oil Board, No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000, 
      Kajang, Selangor, Malaysia. ssleow@mpob.gov.my.
FAU - Khoo, Jia-Shiun
AU  - Khoo JS
AD  - Codon Genomics Sdn Bhd, No. 26, Jalan Dutamas 7, Taman Dutamas Balakong, 43200, 
      Seri Kembangan, Selangor, Malaysia.
FAU - Ng, Siuk-Mun
AU  - Ng SM
AD  - Codon Genomics Sdn Bhd, No. 26, Jalan Dutamas 7, Taman Dutamas Balakong, 43200, 
      Seri Kembangan, Selangor, Malaysia.
FAU - Lee, Wei-Kang
AU  - Lee WK
AD  - Codon Genomics Sdn Bhd, No. 26, Jalan Dutamas 7, Taman Dutamas Balakong, 43200, 
      Seri Kembangan, Selangor, Malaysia.
FAU - Hoh, Chee-Choong
AU  - Hoh CC
AD  - Codon Genomics Sdn Bhd, No. 26, Jalan Dutamas 7, Taman Dutamas Balakong, 43200, 
      Seri Kembangan, Selangor, Malaysia.
FAU - Fairus, Syed
AU  - Fairus S
AD  - Malaysian Palm Oil Board, No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000, 
      Kajang, Selangor, Malaysia.
FAU - Sambanthamurthi, Ravigadevi
AU  - Sambanthamurthi R
AD  - Malaysian Palm Oil Board, No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000, 
      Kajang, Selangor, Malaysia.
AD  - Academy of Sciences Malaysia, Level 20, West Wing, MATRADE Tower, Jalan Sultan 
      Haji Ahmad Shah, Off Jalan Tuanku Abdul Halim, 50480, Kuala Lumpur, Malaysia.
FAU - Hayes, K C
AU  - Hayes KC
AD  - Brandeis University, 415 South Street, Waltham, MA, 02454, USA.
LA  - eng
GR  - PROFENOLIS (2011101805)/Eleventh Malaysia Plan (RMK11)/
GR  - Foster Biomedical Research Laboratory funds for research and teaching/Brandeis 
      University/
PT  - Journal Article
DEP - 20231215
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (Insulin)
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - Male
MH  - *Insulin
MH  - Murinae/genetics
MH  - *Diabetes Mellitus, Type 2/genetics
MH  - Circadian Rhythm/genetics
OTO - NOTNLM
OT  - African grass rat
OT  - Circadian rhythm
OT  - Metabolic syndrome
OT  - Type 2 diabetes mellitus
OT  - Water-Soluble Palm Fruit Extract
OT  - Whole genome sequencing
EDAT- 2023/12/15 12:43
MHDA- 2024/03/11 06:44
CRDT- 2023/12/15 11:06
PHST- 2023/08/23 00:00 [received]
PHST- 2023/12/05 00:00 [accepted]
PHST- 2024/03/11 06:44 [medline]
PHST- 2023/12/15 12:43 [pubmed]
PHST- 2023/12/15 11:06 [entrez]
AID - 10.1007/s10709-023-00202-z [pii]
AID - 10.1007/s10709-023-00202-z [doi]
PST - ppublish
SO  - Genetica. 2024 Feb;152(1):11-29. doi: 10.1007/s10709-023-00202-z. Epub 2023 Dec 
      15.