PMID- 38104433
OWN - NLM
STAT- MEDLINE
DCOM- 20240110
LR  - 20240116
IS  - 1878-3252 (Electronic)
IS  - 0946-672X (Linking)
VI  - 82
DP  - 2024 Mar
TI  - Heavy metal levels are positively associated with serum periostin and miRNA-125b 
      levels, but inversely associated with miRNA-26a levels in pediatric asthma cases. 
      A case-control study.
PG  - 127364
LID - S0946-672X(23)00240-7 [pii]
LID - 10.1016/j.jtemb.2023.127364 [doi]
AB  - BACKGROUND: The study investigated heavy metals levels [urinary cadmium (U-Cd), 
      erythrocytic cadmium (E-Cd), urinary arsenic (U-As), and whole blood lead 
      (WB-Pb)] in children with bronchial asthma (BA) and tested their associations 
      with serum periostin, miRNA-125b and miRNA-26a levels, and with asthma severity 
      clinically and laboratory [blood eosinophils count (BEC) and serum total 
      immunoglobin E (IgE)]. Also, we tested cut-off points, for the studied 
      parameters, to distinguish BA cases from healthy children. METHODS: This 
      case-control study included 158 children divided into control group; n = 72 and 
      BA group; n = 86. Heavy metals were measured by an inductively coupled 
      plasma-optical emission spectrophotometer. Serum periostin and IgE levels were 
      measured by their corresponding ELISA kits. miRNAs relative expressions were 
      estimated by RT-qPCR using the 2(-DeltaDeltaCT) method. RESULTS: Heavy metals, serum 
      periostin, and miR-125b levels were significantly high in BA group (p < 0.001). 
      Heavy metals levels correlated positively with serum periostin, miR-125b and IgE 
      levels, BEC, and asthma severity. The reverse was observed regarding serum 
      miR-26a levels. Receiver operating characteristics (ROC) curve analysis showed 
      good to excellent abilities of U-Cd, E-Cd, U-As, WB-Pb, serum periostin, 
      miRNA-125b, and miRNA - 26a, and total IgE levels to distinguish BA cases from 
      healthy children. CONCLUSIONS: Heavy metal toxicity in children is associated 
      with BA severity, increased serum periostin and miRNA-125b levels, and decreased 
      miRNA-26a levels. Specific measures to reduce children's exposure to heavy metals 
      should be taken. Future research should consider blocking miRNA-125b action or 
      enhancing miRNA-26a action to manage BA cases.
CI  - Copyright (c) 2023 Elsevier GmbH. All rights reserved.
FAU - Mohany, Khalid M
AU  - Mohany KM
AD  - Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, 
      Assiut University, Assiut 71515, Egypt. Electronic address: 
      khalidmohany@aun.edu.eg.
FAU - Gamal, Yasser
AU  - Gamal Y
AD  - Department of Pediatrics, Faculty of Medicine, Assiut University, Assiut 71515, 
      Egypt.
FAU - Abdel Raheem, Yaser F
AU  - Abdel Raheem YF
AD  - Department of Pediatrics, Faculty of Medicine, Assiut University, Assiut 71515, 
      Egypt.
LA  - eng
PT  - Journal Article
DEP - 20231212
PL  - Germany
TA  - J Trace Elem Med Biol
JT  - Journal of trace elements in medicine and biology : organ of the Society for 
      Minerals and Trace Elements (GMS)
JID - 9508274
RN  - 0 (MicroRNAs)
RN  - 00BH33GNGH (Cadmium)
RN  - 0 (Periostin)
RN  - 2P299V784P (Lead)
RN  - 0 (Metals, Heavy)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Child
MH  - Humans
MH  - *MicroRNAs
MH  - Cadmium
MH  - Periostin
MH  - Case-Control Studies
MH  - Lead
MH  - *Asthma
MH  - *Metals, Heavy
MH  - Immunoglobulin E
MH  - Biomarkers
OTO - NOTNLM
OT  - Bronchial asthma
OT  - Heavy metals
OT  - MiRNA-125b
OT  - MiRNA-26a
OT  - Periostin
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/12/18 00:42
MHDA- 2024/01/10 06:42
CRDT- 2023/12/17 18:07
PHST- 2023/08/10 00:00 [received]
PHST- 2023/12/06 00:00 [revised]
PHST- 2023/12/11 00:00 [accepted]
PHST- 2024/01/10 06:42 [medline]
PHST- 2023/12/18 00:42 [pubmed]
PHST- 2023/12/17 18:07 [entrez]
AID - S0946-672X(23)00240-7 [pii]
AID - 10.1016/j.jtemb.2023.127364 [doi]
PST - ppublish
SO  - J Trace Elem Med Biol. 2024 Mar;82:127364. doi: 10.1016/j.jtemb.2023.127364. Epub 
      2023 Dec 12.