PMID- 38104881 OWN - NLM STAT- MEDLINE DCOM- 20240117 LR - 20240117 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 322 DP - 2024 Mar 25 TI - Exploring the hypoglycemic mechanism of chlorogenic acids from Pyrrosia petiolosa (Christ) Ching on type 2 diabetes mellitus based on network pharmacology and transcriptomics strategy. PG - 117580 LID - S0378-8741(23)01450-2 [pii] LID - 10.1016/j.jep.2023.117580 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Pyrrosia petiolosa (Christ) Ching (YBSW) is a Traditional Chinese medicine rich in chlorogenic acids. It is an important component in many Traditional Chinese medicinal hypoglycemic formulas and is commonly used by the Miao people to treat diabetes with good efficacy. Our previous research has suggested that chlorogenic acids may be the active ingredients in YBSW. AIM OF THE STUDY: To explore the mechanisms underlying the anti-type 2 diabetes mellitus (T2DM) hypoglycemic effects of chlorogenic acids contained in YBSW. MATERIALS AND METHODS: In vivo experiments, hematoxylin-eosin staining (HE) staining, and immunohistochemistry (IHC) were used to determine the effects of chlorogenic acids contained in YBSW in rats. mRNA expression profiling, microarray analysis, and network pharmacology were used to analyze the underlying mechanisms of the effects. Finally, apoptosis and changes in the related pathways were evaluated in vitro using a 3-(4,5-dimethyl-2-thia-zolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay, quantitative real-time polymerase chain reaction, immunofluorescence (IF) assessment, and flow cytometry. RESULTS: After the administration of isochlorogenic acid B, the levels of triglycerides, serum total cholesterol, and fasting blood glucose significantly decreased. HE and IHC staining revealed that isochlorogenic acid B significantly increased insulin expression in islet cells. Using network pharmacology and RNA-seq Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, we screened the advanced glycation end products-receptor for advanced glycation end products (AGE-RAGE) signaling pathway. We also verified that YBSW and its chlorogenic acid can inhibit apoptosis and downregulate the expression of related mRNA in the AGE-RAGE pathway in RIN-m5f cells. CONCLUSIONS: YBSW exhibits a significant hypoglycemic effect, with chlorogenic acid being an effective component. The therapeutic effect of chlorogenic acids contained in YBSW is mainly realized by promoting insulin secretion and pancreatic tissue repair. Moreover, YBSW substantially mitigates apoptosis via the AGE-RAGE pathway in T2DM. CI - Copyright (c) 2023 Elsevier B.V. All rights reserved. FAU - He, Hanjiao AU - He H AD - College of Pharmacy, Guizhou University of Traditional Chinese Medicine, No. 4 Dongqingnan Road, Huaxi District, Guiyang, Guizhou 550025, PR China. FAU - Wei, Qing AU - Wei Q AD - College of Pharmacy, Guizhou University of Traditional Chinese Medicine, No. 4 Dongqingnan Road, Huaxi District, Guiyang, Guizhou 550025, PR China. FAU - Chang, Jiao AU - Chang J AD - College of Pharmacy, Guizhou University of Traditional Chinese Medicine, No. 4 Dongqingnan Road, Huaxi District, Guiyang, Guizhou 550025, PR China. FAU - Yi, Xu AU - Yi X AD - Second Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, No. 32 Feishan Road, Nanming District, Guiyang, Guizhou 550002, PR China. FAU - Yu, Xiang AU - Yu X AD - College of Pharmacy, Guizhou University of Traditional Chinese Medicine, No. 4 Dongqingnan Road, Huaxi District, Guiyang, Guizhou 550025, PR China. FAU - Luo, Guoyong AU - Luo G AD - College of Pharmacy, Guizhou University of Traditional Chinese Medicine, No. 4 Dongqingnan Road, Huaxi District, Guiyang, Guizhou 550025, PR China. FAU - Li, Xinfeng AU - Li X AD - College of Pharmacy, Guizhou University of Traditional Chinese Medicine, No. 4 Dongqingnan Road, Huaxi District, Guiyang, Guizhou 550025, PR China. Electronic address: 975155325@qq.com. FAU - Yang, Wude AU - Yang W AD - College of Pharmacy, Guizhou University of Traditional Chinese Medicine, No. 4 Dongqingnan Road, Huaxi District, Guiyang, Guizhou 550025, PR China. Electronic address: yangwude476@gzy.edu.cn. FAU - Long, Yi AU - Long Y AD - College of Pharmacy, Guizhou University of Traditional Chinese Medicine, No. 4 Dongqingnan Road, Huaxi District, Guiyang, Guizhou 550025, PR China. Electronic address: longyi621@gzy.edu.cn. LA - eng PT - Journal Article DEP - 20231216 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - E57A0DKE0B (3,4-di-O-caffeoylquinic acid) RN - 318ADP12RI (Chlorogenic Acid) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Hypoglycemic Agents) RN - 0 (RNA, Messenger) SB - IM MH - Animals MH - Rats MH - Chlorogenic Acid/pharmacology/therapeutic use MH - *Diabetes Mellitus, Type 2/drug therapy/genetics MH - *Drugs, Chinese Herbal/pharmacology/therapeutic use MH - Gene Expression Profiling MH - Hypoglycemic Agents/pharmacology/therapeutic use MH - Molecular Docking Simulation MH - Network Pharmacology MH - RNA, Messenger OTO - NOTNLM OT - Chlorogenic acids OT - Mechanism OT - Network pharmacology OT - Pyrrosia petiolosa (Christ) Ching OT - T2DM OT - Transcriptomics strategy COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/12/18 00:42 MHDA- 2024/01/15 12:43 CRDT- 2023/12/17 19:28 PHST- 2023/09/17 00:00 [received] PHST- 2023/11/05 00:00 [revised] PHST- 2023/12/10 00:00 [accepted] PHST- 2024/01/15 12:43 [medline] PHST- 2023/12/18 00:42 [pubmed] PHST- 2023/12/17 19:28 [entrez] AID - S0378-8741(23)01450-2 [pii] AID - 10.1016/j.jep.2023.117580 [doi] PST - ppublish SO - J Ethnopharmacol. 2024 Mar 25;322:117580. doi: 10.1016/j.jep.2023.117580. Epub 2023 Dec 16.