PMID- 38110097
OWN - NLM
STAT- MEDLINE
DCOM- 20240118
LR  - 20240118
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 912
DP  - 2024 Feb 20
TI  - Study on epigenotoxicity, sex hormone synthesis, and DNA damage of benzo[a]pyrene 
      in the testis of male Ruditapes philippinarum.
PG  - 169340
LID - S0048-9697(23)07970-6 [pii]
LID - 10.1016/j.scitotenv.2023.169340 [doi]
AB  - Research on the mechanisms of reproductive toxicity caused by persistent organic 
      pollutants (POPs) in marine animals has received significant attention. One group 
      of typical POPs, called polycyclic aromatic hydrocarbons (PAHs), has been found 
      to cause various reproductive toxicities in aquatic organisms, including 
      epigenotoxicity, reproductive endocrine disruption, DNA damage effects and other 
      reproductive toxicity, thereby affecting gonadal development. Interestingly, male 
      aquatic animals are more susceptible to the disturbance and toxicity of 
      environmental pollutants. However, current studies primarily focus on 
      vertebrates, leaving a large gap in our understanding of the reproductive 
      toxicity and mechanisms of PAHs interference in marine invertebrates. In this 
      study, male Ruditapes philippinarum was used as an experimental subject to 
      investigate reproduction-related indexes in clams under the stress of 
      benzo[a]pyrene (B[a]P) at different concentrations (0, 0.8, 4 and 20 mug/L) during 
      the proliferative, growth, maturity, and spawning period. We analyzed the 
      molecular mechanisms of reproductive toxicity caused by PAHs in marine bivalves, 
      specifically epigenotoxicity, reproductive endocrine disruption, and gonadal 
      damage-apoptotic effect. The results suggest that DNA methylation plays a crucial 
      role in mediating B[a]P-induced reproductive toxicity in male R. philippinarum. 
      B[a]P may affect sex hormone levels, impede spermatogenesis and testis 
      development in clams, by inhibiting the steroid hormone synthesis pathway and 
      downregulating genes critical for cell proliferation, testis development, and 
      spermatid expulsion. Moreover, the spermatids of male R. philippinarum were 
      severely impaired under the B[a]P stress, leading to reduced reproductive 
      performance in the clams. These findings contribute to a better understanding of 
      the reproductive toxicity response of male marine invertebrates to POPs stress.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Wang, Qiaoqiao
AU  - Wang Q
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Tang, Jian
AU  - Tang J
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Pan, Luqing
AU  - Pan L
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
FAU - Song, Aimin
AU  - Song A
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Miao, Jingjing
AU  - Miao J
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Zheng, Xin
AU  - Zheng X
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
FAU - Li, Zeyuan
AU  - Li Z
AD  - The Key Laboratory of Mariculture, Ministry of Education, Ocean University of 
      China, Qingdao 266003, PR China.
LA  - eng
PT  - Journal Article
DEP - 20231216
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - Male
MH  - Benzo(a)pyrene/toxicity/metabolism
MH  - Testis
MH  - *Bivalvia/metabolism
MH  - Aquatic Organisms
MH  - Gonadal Steroid Hormones/metabolism
MH  - DNA Damage
MH  - *Water Pollutants, Chemical/analysis
OTO - NOTNLM
OT  - DNA damage repair
OT  - Epigenetic toxicity
OT  - Polycyclic aromatic hydrocarbons
OT  - Reproductive endocrine disruption
OT  - Ruditapes philippinarum
OT  - Testis development
COIS- Declaration of competing interest The authors declare the following financial 
      interests/personal relationships which may be considered as potential competing 
      interests: Luqing Pan reports financial support was provided by Shandong 
      Provincial Natural Science Foundation.
EDAT- 2023/12/19 00:41
MHDA- 2024/01/18 06:43
CRDT- 2023/12/18 19:26
PHST- 2023/10/29 00:00 [received]
PHST- 2023/12/10 00:00 [revised]
PHST- 2023/12/11 00:00 [accepted]
PHST- 2024/01/18 06:43 [medline]
PHST- 2023/12/19 00:41 [pubmed]
PHST- 2023/12/18 19:26 [entrez]
AID - S0048-9697(23)07970-6 [pii]
AID - 10.1016/j.scitotenv.2023.169340 [doi]
PST - ppublish
SO  - Sci Total Environ. 2024 Feb 20;912:169340. doi: 10.1016/j.scitotenv.2023.169340. 
      Epub 2023 Dec 16.