PMID- 38111211
OWN - NLM
STAT- MEDLINE
DCOM- 20240131
LR  - 20240206
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 135
IP  - 1
DP  - 2024 Jan 2
TI  - Effect of (in)organic additives on microbially induced calcium carbonate 
      precipitation.
LID - lxad309 [pii]
LID - 10.1093/jambio/lxad309 [doi]
AB  - AIM: This study aimed to understand the morphological effects of (in)organic 
      additives on microbially induced calcium carbonate precipitation (MICP). METHODS 
      AND RESULTS: MICP was monitored in real time in the presence of (in)organic 
      additives: bovine serum albumin (BSA), biofilm surface layer protein A (BslA), 
      magnesium chloride (MgCl2), and poly-l-lysine. This monitoring was carried out 
      using confocal microscopy to observe the formation of CaCO3 from the point of 
      nucleation, in comparison to conditions without additives. Complementary 
      methodologies, namely scanning electron microscopy, energy-dispersive X-ray 
      spectroscopy and X-ray diffraction, were employed to assess the visual 
      morphology, elemental composition, and crystalline structures of CaCO3, 
      respectively, following the crystals' formation. The results demonstrated that in 
      the presence of additives, more CaCO3 crystals were produced at 100 min compared 
      to the reaction without additives. The inclusion of BslA resulted in larger 
      crystals than reactions containing other additives, including MgCl2. BSA induced 
      a significant number of crystals from the early stages of the reaction (20 min) 
      but did not have a substantial impact on crystal size compared to conditions 
      without additives. All additives led to a higher content of calcite compared to 
      vaterite after a 24-h reaction, with the exception of MgCl2, which produced a 
      substantial quantity of magnesium calcite. CONCLUSIONS: The work demonstrates the 
      effect of several (in)organic additives on MICP and sets the stage for further 
      research to understand additive effects on MICP to achieve controlled CaCO3 
      precipitation.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of Applied 
      Microbiology International.
FAU - Haystead, Jamie
AU  - Haystead J
AD  - Hub for Biotechnology in the Built Environment, Department of Applied Sciences, 
      Northumbria University, Ellison Place, Newcastle upon Tyne NE1 8ST, United 
      Kingdom.
FAU - Gilmour, Katie
AU  - Gilmour K
AD  - Hub for Biotechnology in the Built Environment, Department of Applied Sciences, 
      Northumbria University, Ellison Place, Newcastle upon Tyne NE1 8ST, United 
      Kingdom.
FAU - Sherry, Angela
AU  - Sherry A
AD  - Hub for Biotechnology in the Built Environment, Department of Applied Sciences, 
      Northumbria University, Ellison Place, Newcastle upon Tyne NE1 8ST, United 
      Kingdom.
FAU - Dade-Robertson, Martyn
AU  - Dade-Robertson M
AD  - Hub for Biotechnology in the Built Environment, School of Architecture, Planning 
      and Landscape, The Quadrangle, Newcastle University, Newcastle upon Tyne NE1 7RU, 
      United Kingdom.
AD  - Hub for Biotechnology in the Built Environment, Department of Architecture and 
      Built Environment, Northumbria University, NE1 8ST, United Kingdom.
FAU - Zhang, Meng
AU  - Zhang M
AUID- ORCID: 0000-0003-1089-6464
AD  - Hub for Biotechnology in the Built Environment, Department of Applied Sciences, 
      Northumbria University, Ellison Place, Newcastle upon Tyne NE1 8ST, United 
      Kingdom.
LA  - eng
GR  - EP/R003629/1/Engineering and Physical Sciences Research Council/
PT  - Journal Article
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - H0G9379FGK (Calcium Carbonate)
RN  - 02F3473H9O (Magnesium Chloride)
SB  - IM
MH  - *Calcium Carbonate/metabolism
MH  - Magnesium Chloride/metabolism
MH  - *Sporosarcina/metabolism
MH  - Chemical Precipitation
MH  - Microscopy, Electron, Scanning
OTO - NOTNLM
OT  - Sporosarcina pasteurii
OT  - (in)organic additives
OT  - CaCO3 morphology
OT  - microbially induced calcium carbonate precipitation (MICP)
EDAT- 2023/12/19 06:42
MHDA- 2024/01/31 06:42
CRDT- 2023/12/19 02:06
PHST- 2023/10/07 00:00 [received]
PHST- 2023/11/28 00:00 [revised]
PHST- 2023/12/15 00:00 [accepted]
PHST- 2024/01/31 06:42 [medline]
PHST- 2023/12/19 06:42 [pubmed]
PHST- 2023/12/19 02:06 [entrez]
AID - 7478027 [pii]
AID - 10.1093/jambio/lxad309 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2024 Jan 2;135(1):lxad309. doi: 10.1093/jambio/lxad309.