PMID- 38112810
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231222
IS  - 2191-0855 (Print)
IS  - 2191-0855 (Electronic)
IS  - 2191-0855 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Dec 19
TI  - Klebsiella michiganensis: a nitrogen-fixing endohyphal bacterium from Ustilago 
      maydis.
PG  - 146
LID - 10.1186/s13568-023-01618-8 [doi]
LID - 146
AB  - Ustilago maydis is a pathogenic fungus in Basidiomycota causing corn smut 
      disease. A strain of U. maydis YZZF202006 was isolated from the tumor of corn 
      smut collected from Jingzhou city in China. The intracellular bacteria were 
      confirmed inner hyphal of the strain YZZF202006 by PCR amplification and 
      fluorescence in situ hybridization (FISH) and SYTO-9. An endohyphal bacterium 
      YZUMF202001 was isolated from the protoplasts of the strain YZZF202006. It was 
      gram-negative, short rod-shaped with smooth light yellow colony. The endohyphal 
      bacterium was genomic evidenced as Klebsiella michiganensis on the basis of 
      average nucleotide identity (ANI) analysis and the phylogram. Then K. 
      michiganensis was GFP-Labeled and reintroduced into U. maydis, which confirmed 
      the bacterium can live in hyphae of U.maydis. The bacterium can grow on N-free 
      culture media. Its nitrogenase activity was reached av. 646.25 +/- 38.61 
      nmol.mL(- 1).h(- 1) C(2)H(4) by acetylene reduction assay. A cluster of nitrogen 
      fixation genes (nifJHDKTXENXUSVWZMFLABQ) was found from its genome. The 
      endohyphal K. michiganensis may play an important role to help nitrogen fixation 
      for fungi in the future.
CI  - (c) 2023. The Author(s).
FAU - Liang, Pengyu
AU  - Liang P
AD  - Department of Plant Protection, College of Agriculture, Yangtze University, 
      Jingzhou, 434025, China.
FAU - Jiang, Jianwei
AU  - Jiang J
AD  - Department of Plant Protection, College of Agriculture, Yangtze University, 
      Jingzhou, 434025, China.
FAU - Sun, Zhengxiang
AU  - Sun Z
AD  - Department of Plant Protection, College of Agriculture, Yangtze University, 
      Jingzhou, 434025, China.
FAU - Li, Yanyan
AU  - Li Y
AD  - Tobacco Research Institute of Hubei Province, Wuhan, 430000, China.
FAU - Yang, Chunlei
AU  - Yang C
AD  - Tobacco Research Institute of Hubei Province, Wuhan, 430000, China. 
      ycl193737@163.com.
FAU - Zhou, Yi
AU  - Zhou Y
AD  - Department of Plant Protection, College of Agriculture, Yangtze University, 
      Jingzhou, 434025, China. zhouyi@yangtzeu.edu.cn.
LA  - eng
GR  - 2020BBA051/the key research and development plan project of Hubei Province/
GR  - 110202101013 (XJ-5）/Science and technology project of China National Tobacco 
      Corporation/
GR  - 027Y2022-023/Key science and technology projects of China National Tobacco 
      Corporation in Hubei Province/
PT  - Journal Article
DEP - 20231219
PL  - Germany
TA  - AMB Express
JT  - AMB Express
JID - 101561785
PMC - PMC10730499
OTO - NOTNLM
OT  - Endohyphal bacterium
OT  - Klebsiella michiganensis
OT  - Nitrogen fixation
OT  - Ustilago maydis
COIS- The authors declare no competing interests.
EDAT- 2023/12/19 19:54
MHDA- 2023/12/19 19:55
PMCR- 2023/12/19
CRDT- 2023/12/19 11:17
PHST- 2023/01/26 00:00 [received]
PHST- 2023/10/03 00:00 [accepted]
PHST- 2023/12/19 19:55 [medline]
PHST- 2023/12/19 19:54 [pubmed]
PHST- 2023/12/19 11:17 [entrez]
PHST- 2023/12/19 00:00 [pmc-release]
AID - 10.1186/s13568-023-01618-8 [pii]
AID - 1618 [pii]
AID - 10.1186/s13568-023-01618-8 [doi]
PST - epublish
SO  - AMB Express. 2023 Dec 19;13(1):146. doi: 10.1186/s13568-023-01618-8.