PMID- 38114120
OWN - NLM
STAT- MEDLINE
DCOM- 20231221
LR  - 20231221
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 48
IP  - 19
DP  - 2023 Oct
TI  - [Diosgenin alleviates NAFLD induced by a high-fat diet in rats via 
      mTOR/SREBP-1c/HSP60/MCAD/SCAD signaling pathway].
PG  - 5304-5314
LID - 10.19540/j.cnki.cjcmm.20230601.705 [doi]
AB  - This study aims to observe the effects of diosgenin on the expression of 
      mammalian target of rapamycin(mTOR), sterol regulatory element-binding 
      protein-1c(SREBP-1c), heat shock protein 60(HSP60), medium-chain acyl-CoA 
      dehydrogenase(MCAD), and short-chain acyl-CoA dehydrogenase(SCAD) in the liver 
      tissue of the rat model of non-alcoholic fatty liver disease(NAFLD) and explore 
      the mechanism of diosgenin in alleviating NAFLD. Forty male SD rats were 
      randomized into five groups: a control group, a model group, low-(150 
      mg.kg~(-1).d~(-1)) and high-dose(300 mg.kg~(-1).d~(-1)) diosgenin groups, and a 
      simvastatin(4 mg.kg~(-1).d~(-1)) group. The rats in the control group were fed 
      with a normal diet, while those in the other four groups were fed with a high-fat 
      diet. After feeding for 8 weeks, the body weight of rats in the high-fat diet 
      groups increased significantly. After that, the rats were administrated with the 
      corresponding dose of diosgenin or simvastatin by gavage every day for 8 weeks. 
      The levels of triglyceride(TG), total cholesterol(TC), alanine transaminase(ALT), 
      and aspartate transaminase(AST) in the serum were determined by the biochemical 
      method. The levels of TG and TC in the liver were measured by the enzyme method. 
      Oil-red O staining was employed to detect the lipid accumulation, and 
      hematoxylin-eosin(HE) staining to detect the pathological changes in the liver 
      tissue. The mRNA and protein levels of mTOR, SREBP-1c, HSP60, MCAD, and SCAD in 
      the liver tissue of rats were determined by real-time fluorescence quantitative 
      polymerase chain reaction(RT-qPCR) and Western blot, respectively. Compared with 
      the control group, the model group showed increased body weight, food uptake, 
      liver index, TG, TC, ALT, and AST levels in the serum, TG and TC levels in the 
      liver, lipid deposition in the liver, obvious hepatic steatosis, up-regulated 
      mRNA and protein expression levels of mTOR and SREBP-1c, and down-regulated mRNA 
      and protein expression levels of HSP60, MCAD, and SCAD. Compared with the model 
      group, the rats in each treatment group showed obviously decreased body weight, 
      food uptake, liver index, TG, TC, ALT, and AST levels in the serum, TG and TC 
      levels in the liver, lessened lipid deposition in the liver, ameliorated hepatic 
      steatosis, down-regulated mRNA and protein le-vels of mTOR and SREBP-1c, and 
      up-regulated mRNA and protein levels of HSP60, MCAD, and SCAD. The high-dose 
      diosgenin outperformed the low-dose diosgenin and simvastatin. Diosgenin may 
      prevent and treat NAFLD by inhibiting the expression of mTOR and SREBP-1c and 
      promoting the expression of HSP60, MCAD, and SCAD to reduce lipid synthesis, 
      improving mitochondrial function, and promoting fatty acid beta oxidation in the 
      liver.
FAU - Chen, Su-Wen
AU  - Chen SW
AD  - Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
FAU - Yin, Guo-Liang
AU  - Yin GL
AD  - Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
FAU - Song, Chao-Yuan
AU  - Song CY
AD  - Zibo Central Hospital Zibo 255000, China.
FAU - Meng, De-Cheng
AU  - Meng DC
AD  - Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
FAU - Yu, Wen-Fei
AU  - Yu WF
AD  - Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
FAU - Zhang, Xin
AU  - Zhang X
AD  - Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
FAU - Feng, Ya-Nan
AU  - Feng YN
AD  - Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
FAU - Liang, Peng-Peng
AU  - Liang PP
AD  - Shandong University of Traditional Chinese Medicine Ji'nan 250014, China.
FAU - Zhang, Feng-Xia
AU  - Zhang FX
AD  - Affiliated Hospital of Shandong University of Traditional Chinese Medicine Ji'nan 
      250014, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Sterol Regulatory Element Binding Protein 1)
RN  - K49P2K8WLX (Diosgenin)
RN  - 0 (Chaperonin 60)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - 0 (Triglycerides)
RN  - 0 (RNA, Messenger)
RN  - AGG2FN16EV (Simvastatin)
RN  - EC 2.7.1.1 (mTOR protein, rat)
SB  - IM
MH  - Rats
MH  - Male
MH  - Animals
MH  - *Non-alcoholic Fatty Liver Disease/etiology/genetics
MH  - Sterol Regulatory Element Binding Protein 1/metabolism
MH  - Diet, High-Fat/adverse effects
MH  - *Diosgenin/metabolism
MH  - Chaperonin 60/metabolism/pharmacology/therapeutic use
MH  - Rats, Sprague-Dawley
MH  - Liver
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Triglycerides
MH  - RNA, Messenger/metabolism
MH  - Simvastatin/metabolism/pharmacology/therapeutic use
MH  - Body Weight
MH  - Lipid Metabolism
MH  - Mammals/genetics/metabolism
OTO - NOTNLM
OT  - diosgenin
OT  - fatty acid beta oxidation
OT  - lipid synthesis
OT  - mitochondrial function
OT  - non-alcoholic fatty liver disease
EDAT- 2023/12/20 06:41
MHDA- 2023/12/21 06:42
CRDT- 2023/12/19 20:43
PHST- 2023/12/21 06:42 [medline]
PHST- 2023/12/20 06:41 [pubmed]
PHST- 2023/12/19 20:43 [entrez]
AID - 10.19540/j.cnki.cjcmm.20230601.705 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2023 Oct;48(19):5304-5314. doi: 
      10.19540/j.cnki.cjcmm.20230601.705.