PMID- 38114178
OWN - NLM
STAT- MEDLINE
DCOM- 20231221
LR  - 20231221
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 48
IP  - 21
DP  - 2023 Nov
TI  - [Neuroprotective effect and mechanism of Zuogui Jiangtang Jieyu Formula on 
      diabetes mellitus complicated with depression model rats based on CX3CL1-CX3CR1 
      axis].
PG  - 5822-5829
LID - 10.19540/j.cnki.cjcmm.20230605.406 [doi]
AB  - Based on the CX3C chemokine ligand 1(CX3CL1)-CX3C chemokine receptor 1(CX3CR1) 
      axis, this study explored the potential mechanism by which Zuogui Jiangtang Jieyu 
      Formula(ZGJTJY) improved neuroinflammation and enhanced neuroprotective effect in 
      a rat model of diabetes mellitus complicated with depression(DD). The DD rat 
      model was established by feeding a high-fat diet combined with 
      streptozotocin(STZ) intraperitoneal injection for four weeks and chronic 
      unpredictable mild stress(CUMS) combined with isolated cage rearing for five 
      weeks. The rats were divided into a control group, a model group, a positive 
      control group, an inhibitor group, and a ZGJTJY group. The open field test and 
      forced swimming test were used to assess the depression-like behaviors of the 
      rats. Enzyme-linked immunosorbent assay(ELISA) was performed to measure the 
      expression levels of the pro-inflammatory cytokines interleukin-1beta(IL-1beta) and 
      tumor necrosis factor-alpha(TNF-alpha) in plasma. Immunofluorescence staining was used to 
      detect the expression of ionized calcium-binding adapter molecule 1(Iba1), 
      postsynaptic density protein-95(PSD95), and synapsin-1(SYN1) in the hippocampus. 
      Hematoxylin-eosin(HE) staining, Nissl staining, and TdT-mediated dUTP nick end 
      labeling(TUNEL) fluorescence staining were performed to assess hippocampal 
      neuronal damage. Western blot was used to measure the expression levels of 
      CX3CL1, CX3CR1, A2A adenosine receptor(A2AR), glutamate receptor 2A(NR2A), 
      glutamate receptor 2B(NR2B), and brain-derived neurotrophic factor(BDNF) in the 
      hippocampus. Compared with the model group, the ZGJTJY group showed improved 
      depression-like behaviors in DD rats, enhanced neuroprotective effect, increased 
      expression of PSD95, SYN1, and BDNF(P<0.01), and decreased expression of Iba1, 
      IL-1beta, and TNF-alpha(P<0.01), as well as the expression of CX3CL1, CX3CR1, A2AR, 
      NR2A, and NR2B(P<0.01). These results suggest that ZGJTJY may exert its 
      neuroprotective effect by inhibiting the CX3CL1-CX3CR1 axis and activation of 
      hippocampal microglia, thereby improving neuroinflammation and abnormal 
      activation of N-methyl-D-aspartate receptor(NMDAR) subunits, and ultimately 
      enhancing the expression of synaptic-related proteins PSD95, SYN1, and BDNF in 
      the hippocampus.
FAU - Li, Ping
AU  - Li P
AD  - Technology Innovation Center,Hunan University of Chinese Medicine Changsha 
      410208,China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Technology Innovation Center,Hunan University of Chinese Medicine Changsha 
      410208,China.
FAU - Zou, Man-Shu
AU  - Zou MS
AD  - Technology Innovation Center,Hunan University of Chinese Medicine Changsha 
      410208,China Hunan Provincial Key Laboratory of Prevention and Treatment of 
      Depressive Diseases with Traditional Chinese Medicine Changsha 410208,China.
FAU - Wang, Ting-Ting
AU  - Wang TT
AD  - Technology Innovation Center,Hunan University of Chinese Medicine Changsha 
      410208,China.
FAU - Guo, Hai-Peng
AU  - Guo HP
AD  - Technology Innovation Center,Hunan University of Chinese Medicine Changsha 
      410208,China.
FAU - Ren, Ting-Ting
AU  - Ren TT
AD  - Technology Innovation Center,Hunan University of Chinese Medicine Changsha 
      410208,China.
FAU - He, Ying
AU  - He Y
AD  - Technology Innovation Center,Hunan University of Chinese Medicine Changsha 
      410208,China.
FAU - Wang, Hua
AU  - Wang H
AD  - the First Affiliated Hospital of Hunan University of Chinese Medicine Changsha 
      410007,China.
FAU - Wang, Yu-Hong
AU  - Wang YH
AD  - Technology Innovation Center,Hunan University of Chinese Medicine Changsha 
      410208,China Hunan Provincial Key Laboratory of Prevention and Treatment of 
      Depressive Diseases with Traditional Chinese Medicine Changsha 410208,China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (zuogui)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Receptors, Glutamate)
RN  - 0 (CX3CR1 protein, rat)
RN  - 0 (CX3C Chemokine Receptor 1)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Depression/drug therapy
MH  - Brain-Derived Neurotrophic Factor
MH  - *Neuroprotective Agents
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Neuroinflammatory Diseases
MH  - *Diabetes Mellitus
MH  - Receptors, Glutamate
MH  - CX3C Chemokine Receptor 1/genetics
OTO - NOTNLM
OT  - CX3C chemokine ligand 1(CX3CL1)
OT  - CX3C chemokine receptor 1(CX3CR1)
OT  - Zuogui Jiangtang Jieyu Formula
OT  - diabetes mellitus complicated with depression
OT  - synaptic proteins
EDAT- 2023/12/20 06:41
MHDA- 2023/12/21 06:43
CRDT- 2023/12/19 20:44
PHST- 2023/12/21 06:43 [medline]
PHST- 2023/12/20 06:41 [pubmed]
PHST- 2023/12/19 20:44 [entrez]
AID - 10.19540/j.cnki.cjcmm.20230605.406 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2023 Nov;48(21):5822-5829. doi: 
      10.19540/j.cnki.cjcmm.20230605.406.