PMID- 38114180
OWN - NLM
STAT- MEDLINE
DCOM- 20231221
LR  - 20231221
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 48
IP  - 21
DP  - 2023 Nov
TI  - [Effect and mechanism of Jiming Powder on myocardial fibrosis in mice with 
      myocardial infarction].
PG  - 5838-5850
LID - 10.19540/j.cnki.cjcmm.20230605.401 [doi]
AB  - Jiming Powder is a traditional ancient prescription with good therapeutic effect 
      in the treatment of heart failure, but its mechanism lacks further exploration. 
      In this study, a mouse model of coronary artery ligation was used to evaluate the 
      effect and mechanism of Jiming Powder on myocardial fibrosis in mice with 
      myocardial infarction. The study constructed a mouse model of heart failure after 
      myocardial infarction using the method of left anterior descending coronary 
      artery ligation. The efficacy of Jiming Powder was evaluated from multiple 
      angles, including ultrasound imaging, hematoxylin-eosin(HE) staining, Masson 
      staining, Sirius Red staining, and serum myocardial enzyme spectrum detection. 
      Western blot analysis was performed to detect key proteins involved in 
      ventricular remodeling, including transforming growth factor-beta1(TGF-beta1), alpha-smooth 
      muscle actin(alpha-SMA), wingless-type MMTV integration site family member 3a(Wnt3a), 
      beta-catenin, matrix metallopeptidase 2(MMP2), matrix metallopeptidase 3(MMP3), TIMP 
      metallopeptidase inhibitor 1(TIMP1), and TIMP metallopeptidase inhibitor 
      2(TIMP2). The results showed that compared with the model group, the high and 
      low-dose Jiming Powder significantly reduced the left ventricular internal 
      diameter in systole(LVID;s) and diastole(LVID;d), increased the left ventricular 
      ejection fraction(LVEF) and left ventricular fractional shortening(LVFS), 
      effectively improved cardiac function in mice after myocardial infarction, and 
      effectively reduced the levels of myocardial injury markers such as creatine 
      kinase(CK), creatine kinase isoenzyme(CK-MB), and lactic dehydrogenase(LDH), thus 
      protecting ischemic myocardium. HE staining showed that Jiming Powder could 
      attenuate myocardial inflammatory cell infiltration after myocardial infarction. 
      Masson and Sirius Red staining demonstrated that Jiming Powder effectively 
      inhibited myocardial fibrosis, reduced the collagen Ⅰ/Ⅲ ratio in myocardial 
      tissues, and improved collagen remodeling after myocardial infarction. Western 
      blot results showed that Jiming Powder reduced the expression of TGF-beta1, alpha-SMA, 
      Wnt3a, and beta-catenin, decreased the levels of MMP2, MMP3, and TIMP2, and 
      increased the level of TIMP1, suggesting its role in inhibiting cardiac 
      fibroblast transformation, reducing extracellular matrix metabolism in myocardial 
      cells, and lowering collagen Ⅰ and alpha-SMA content, thus exerting an 
      anti-myocardial fibrosis effect after myocardial infarction. This study revealed 
      the role of Jiming Powder in improving ventricular remodeling and treating 
      myocardial infarction, laying the foundation for further research on the 
      pharmacological effect of Jiming Powder.
FAU - Fan, Xin-Yi
AU  - Fan XY
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine 
      Beijing 100029, China.
FAU - Wei, Xiao-Qi
AU  - Wei XQ
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine 
      Beijing 100029, China.
FAU - Zhang, Yun-Yang
AU  - Zhang YY
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine 
      Beijing 100029, China.
FAU - Pu, Hai-Yin
AU  - Pu HY
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine 
      Beijing 100029, China.
FAU - Li, Fang-He
AU  - Li FH
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine 
      Beijing 100029, China.
FAU - Gao, Kuo
AU  - Gao K
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine 
      Beijing 100029, China.
FAU - Yu, Xue
AU  - Yu X
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine 
      Beijing 100029, China.
FAU - Guo, Shu-Zhen
AU  - Guo SZ
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine 
      Beijing 100029, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - 0 (beta Catenin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - 0 (Powders)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.3.2 (Creatine Kinase)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Transforming Growth Factor beta1/metabolism
MH  - Matrix Metalloproteinase 2/metabolism
MH  - beta Catenin/metabolism
MH  - Matrix Metalloproteinase 3/metabolism/therapeutic use
MH  - Powders
MH  - Ventricular Remodeling
MH  - Stroke Volume
MH  - Ventricular Function, Left
MH  - *Myocardial Infarction/drug therapy
MH  - Myocardium/pathology
MH  - *Heart Failure/metabolism
MH  - Collagen/metabolism
MH  - Creatine Kinase
MH  - Fibrosis
OTO - NOTNLM
OT  - Jiming Powder
OT  - heart failue
OT  - myocardial fibrosis
OT  - myocardial infarction
EDAT- 2023/12/20 06:41
MHDA- 2023/12/21 06:43
CRDT- 2023/12/19 20:44
PHST- 2023/12/21 06:43 [medline]
PHST- 2023/12/20 06:41 [pubmed]
PHST- 2023/12/19 20:44 [entrez]
AID - 10.19540/j.cnki.cjcmm.20230605.401 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2023 Nov;48(21):5838-5850. doi: 
      10.19540/j.cnki.cjcmm.20230605.401.