PMID- 38115227
OWN - NLM
STAT- MEDLINE
DCOM- 20240422
LR  - 20240422
IS  - 1537-6524 (Electronic)
IS  - 1537-6516 (Linking)
VI  - 34
IP  - 4
DP  - 2024 May
TI  - Eucalyptol regulates Nrf2 and NF-kB signaling and alleviates gentamicin-induced 
      kidney injury in rats by downregulating oxidative stress, oxidative DNA damage, 
      inflammation, and apoptosis.
PG  - 413-422
LID - 10.1080/15376516.2023.2297234 [doi]
AB  - Gentamicin, an aminoglycoside antibiotic, is nowadays widely used in the 
      treatment of gram-negative microorganisms. The antimicrobial, anti-inflammatory, 
      and antioxidant activities of eucalyptol, a type of saturated monoterpene, have 
      been reported in many studies. The aim of this study was to examine the possible 
      effects of eucalyptol on gentamicin-induced renal toxicity. A total of 32 rats 
      were divided into 4 groups; Control (C), Eucalyptol (EUC), Gentamicin (GEN), and 
      Gentamicin + Eucalyptol (GEN + EUC). In order to induce renal toxicity, 100 mg/kg 
      gentamicin was administered intraperitoneally (i.p.) for 10 consecutive days in 
      the GEN and GEN + EUC groups. EUC and GEN + EUC groups were given 100 mg/kg 
      orally of eucalyptol for 10 consecutive days. Afterwards, rats were euthanized 
      and samples were taken and subjected to histopathological, biochemical, 
      immunohistochemical, and real-time PCR examinations. The blood urea nitrogen 
      (BUN) and creatinine (CRE) levels were significantly decreased in the GEN + EUC 
      group (0.76 and 0.69-fold, respectively) compared to the GEN group. The 
      glutathione peroxidase (GPx) and catalase (CAT) activities were significantly 
      increased in the GEN + EUC group (1.35 and 2.67-fold, respectively) compared to 
      the GEN group. In GEN group, Nuclear factor kappa B (NF-kB), Interleukin 1-beta 
      (IL-1beta), Inducible nitric oxide synthase (iNOS), Tumor necrosis factor-alpha (TNF-alpha), 
      Caspase-3, 8-Hydroxy-2'-deoxyguanosine (8-OHdG) and Nuclear factor erythroid 
      2-related factor (Nrf2) expression levels were found to be quite irregular. 
      GEN + EUC group decreased the expressions of NF-kB, IL-1beta, iNOS, TNF-alpha, 
      Caspase-3, and 8-OHdG (0.55, 0.67, 0.54, 0.54, 0.63 and 0.67-fold, respectively), 
      while it caused increased expression of Nrf2 (3.1 fold). In addition, eucalyptol 
      treatment ameliorated the histopathological changes that occurred with 
      gentamicin. The results of our study show that eucalyptol has anti-inflammatory, 
      antioxidative, antiapoptotic, nephroprotective, and curative effects on 
      gentamicin-induced nephrotoxicity.
FAU - Akcakavak, Gokhan
AU  - Akcakavak G
AUID- ORCID: 0000-0001-5949-4752
AD  - Department of Pathology, Yozgat Bozok University, Yozgat, Turkey.
FAU - Kazak, Filiz
AU  - Kazak F
AUID- ORCID: 0000-0002-9065-394X
AD  - Department of Biochemistry, Hatay Mustafa Kemal University, Hatay, Turkey.
FAU - Karatas, Ozhan
AU  - Karatas O
AUID- ORCID: 0000-0002-2778-8059
AD  - Department of Pathology, Cumhuriyet University, Sivas, Turkey.
FAU - Alakus, Halil
AU  - Alakus H
AUID- ORCID: 0000-0001-9265-2310
AD  - Department of Surgery Hatay Mustafa Kemal University, Hatay, Turkey.
FAU - Alakus, Ibrahim
AU  - Alakus I
AUID- ORCID: 0000-0002-2031-7035
AD  - Department of Surgery Hatay Mustafa Kemal University, Hatay, Turkey.
FAU - Kirgiz, Omer
AU  - Kirgiz O
AUID- ORCID: 0000-0002-0222-1363
AD  - Department of Surgery Hatay Mustafa Kemal University, Hatay, Turkey.
FAU - Celik, Zeynep
AU  - Celik Z
AUID- ORCID: 0000-0002-9667-5728
AD  - Department of Pathology, Selcuk University, Konya, Turkey.
FAU - Yilmaz Deveci, Mehmet Zeki
AU  - Yilmaz Deveci MZ
AUID- ORCID: 0000-0002-9532-247X
AD  - Department of Surgery Hatay Mustafa Kemal University, Hatay, Turkey.
FAU - Ozdemir, Ozgur
AU  - Ozdemir O
AUID- ORCID: 0000-0002-1595-0557
AD  - Department of Pathology, Selcuk University, Konya, Turkey.
FAU - Tuzcu, Mehmet
AU  - Tuzcu M
AUID- ORCID: 0000-0003-3118-1054
AD  - Department of Pathology, Selcuk University, Konya, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20231226
PL  - England
TA  - Toxicol Mech Methods
JT  - Toxicology mechanisms and methods
JID - 101134521
RN  - 0 (NF-kappa B)
RN  - 0 (Gentamicins)
RN  - RV6J6604TK (Eucalyptol)
RN  - EC 3.4.22.- (Caspase 3)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Antioxidants)
RN  - 0 (Anti-Inflammatory Agents)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *NF-kappa B/metabolism
MH  - *Gentamicins/toxicity
MH  - Eucalyptol/metabolism/pharmacology/therapeutic use
MH  - Caspase 3/metabolism
MH  - NF-E2-Related Factor 2/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Kidney
MH  - Oxidative Stress
MH  - Antioxidants/metabolism
MH  - Inflammation/metabolism
MH  - Anti-Inflammatory Agents/pharmacology/therapeutic use
MH  - Apoptosis
OTO - NOTNLM
OT  - Gentamicin
OT  - apoptosis
OT  - eucalyptol
OT  - inflammation
OT  - nephrotoxicity
OT  - oxidative stress
EDAT- 2023/12/20 06:42
MHDA- 2024/04/22 06:42
CRDT- 2023/12/20 00:42
PHST- 2024/04/22 06:42 [medline]
PHST- 2023/12/20 06:42 [pubmed]
PHST- 2023/12/20 00:42 [entrez]
AID - 10.1080/15376516.2023.2297234 [doi]
PST - ppublish
SO  - Toxicol Mech Methods. 2024 May;34(4):413-422. doi: 10.1080/15376516.2023.2297234. 
      Epub 2023 Dec 26.