PMID- 38126537
OWN - NLM
STAT- MEDLINE
DCOM- 20231222
LR  - 20231223
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 56
DP  - 2023
TI  - The level of Tim-3+CD8+ T cells can serve as a potential marker for evaluating 
      the severity of acute graft-versus-host disease after haplo-PBSCT.
PG  - e12997
LID - S0100-879X2023000100696 [pii]
LID - 10.1590/1414-431X2023e12997 [doi]
LID - e12997
AB  - Early and accurate diagnosis of acute graft-versus-host disease (aGVHD) after 
      allogeneic hematopoietic stem cell transplantation is crucial for the prognosis 
      of patients. This study identified a potential biomarker for the severity of 
      aGVHD after human leukocyte antigen (HLA)-haploidentical peripheral blood 
      hematopoietic stem cell transplantation (haplo-PBSCT). We included 20 healthy 
      subjects and 57 patients who underwent haplo-PBSCT. Of these patients, 22 
      developed aGVHD after haplo-PBSCT. The results showed that patients with aGVHD 
      had significantly increased levels of Tim-3+/Perforin+/Granzyme B+CD8+ T cells, 
      but significantly decreased Galectin-9. The differences in Galectin-9 and 
      Tim-3+/Granzyme B+CD8+ T cells between grade I-II aGVHD and III-IV aGVHD were 
      also significant. In vitro, the apoptosis of CD8+ T cells from aGVHD patients was 
      significantly increased after Tim-3/Galectin-9 pathway activation, which 
      decreased Granzyme B secretion. As revealed by univariate analysis, the level of 
      Tim-3+CD8+ T cells was a risk factor for severe aGVHD. ROC analysis demonstrated 
      that high levels of Tim-3+CD8+ T cells had a significant diagnostic value for 
      severe aGVHD, with an area under the curve of 0.854 and cut-off value of 14.155%. 
      In conclusion, the binding of Tim-3 with exogenous Galectin-9 can promote 
      apoptosis of CD8+ T cells and affect the secretion of Granzyme B. Tim-3+CD8+ T 
      cells have the potential to serve as immunological markers for assessing the 
      severity of aGVHD after haplo-PBSCT and identifying patients at a higher risk for 
      severe aGVHD.
FAU - Pang, Nannan
AU  - Pang N
AUID- ORCID: 0000-0003-0975-192X
AD  - Department of Pathology, the First Affiliated Hospital of Shihezi University, 
      Shihezi, Xinjiang, China.
AD  - CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical 
      Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
FAU - Yu, Mingkai
AU  - Yu M
AUID- ORCID: 0000-0001-6046-2846
AD  - School of Public Health, Xinjiang Medical University, Urumqi, China.
FAU - Xu, Jianli
AU  - Xu J
AUID- ORCID: 0000-0002-9538-0370
AD  - Center of Hematology, the First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, China.
FAU - Yuan, Hailong
AU  - Yuan H
AUID- ORCID: 0000-0002-6251-2285
AD  - Center of Hematology, the First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, China.
FAU - Chen, Gang
AU  - Chen G
AUID- ORCID: 0000-0002-6067-1397
AD  - Center of Hematology, the First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, China.
FAU - Wang, Dong
AU  - Wang D
AUID- ORCID: 0009-0002-2670-8616
AD  - Center of Hematology, the First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, China.
FAU - Han, Chunxia
AU  - Han C
AUID- ORCID: 0009-0009-0261-1810
AD  - Center of Hematology, the First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, China.
FAU - Wang, Weiguo
AU  - Wang W
AUID- ORCID: 0000-0003-2688-8557
AD  - Department of Urology, Suzhou Hospital, Affiliated Hospital of Medical School, 
      Nanjing University, Suzhou, China.
FAU - Ding, Jianbing
AU  - Ding J
AUID- ORCID: 0000-0001-5506-7665
AD  - School of Public Health, Xinjiang Medical University, Urumqi, China.
FAU - Jiang, Ming
AU  - Jiang M
AUID- ORCID: 0000-0003-2523-7285
AD  - Center of Hematology, the First Affiliated Hospital of Xinjiang Medical 
      University, Xinjiang Uygur Autonomous Region Research Institute of Hematology, 
      Urumqi, China.
LA  - eng
PT  - Journal Article
DEP - 20231218
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - EC 3.4.21.- (Granzymes)
RN  - 0 (Hepatitis A Virus Cellular Receptor 2)
RN  - 0 (Galectins)
SB  - IM
MH  - Humans
MH  - Granzymes
MH  - Hepatitis A Virus Cellular Receptor 2
MH  - *Hematopoietic Stem Cell Transplantation/adverse effects
MH  - CD8-Positive T-Lymphocytes
MH  - *Graft vs Host Disease/diagnosis/etiology
MH  - Galectins
MH  - Acute Disease
PMC - PMC10729645
EDAT- 2023/12/21 12:41
MHDA- 2023/12/22 06:43
PMCR- 2023/12/18
CRDT- 2023/12/21 08:04
PHST- 2023/08/11 00:00 [received]
PHST- 2023/10/29 00:00 [accepted]
PHST- 2023/12/22 06:43 [medline]
PHST- 2023/12/21 12:41 [pubmed]
PHST- 2023/12/21 08:04 [entrez]
PHST- 2023/12/18 00:00 [pmc-release]
AID - S0100-879X2023000100696 [pii]
AID - 10.1590/1414-431X2023e12997 [doi]
PST - epublish
SO  - Braz J Med Biol Res. 2023 Dec 18;56:e12997. doi: 10.1590/1414-431X2023e12997. 
      eCollection 2023.