PMID- 38126564
OWN - NLM
STAT- MEDLINE
DCOM- 20231227
LR  - 20240114
IS  - 1678-7765 (Electronic)
IS  - 1678-7757 (Print)
IS  - 1678-7757 (Linking)
VI  - 31
DP  - 2023
TI  - Possible role of ALDH1 and CD44 in lip carcinogenesis.
PG  - e20230227
LID - S1678-77572023000100464 [pii]
LID - 10.1590/1678-7757-2023-0227 [doi]
LID - e20230227
AB  - Lip squamous cell carcinoma (LSCC) accounts for 12% of all head and neck cancers. 
      It is caused by chronic exposure to ultraviolet light solar radiation and related 
      to previous actinic cheilitis (AC). This study aimed to investigate the 
      immunostaining of the putative cancer stem cells (CSC) markers ALDH1 and CD44 in 
      AC (n=30) and LSCC (n=20). ALDH1 positivity was found to be statistically higher 
      in LSCC than in AC lesions (p=0.0045), whilst CD44 expression was statistically 
      higher in AC than in LSCC lesions (p=0.0155). ALDH1+ cells in AC lesions were 
      associated with specific clinical features: a younger age (<60 years old), the 
      female gender, white skin, not smoking or consuming alcohol, and a fast 
      evolution, and not associated with the chronic exposure to UV radiation 
      (p<0.0001). CD44 positivity was associated with patients who were male, feoderm, 
      smoked, consumed alcohol, underwent occupational exposure to UV-radiation, and 
      demonstrated lesions with log-time evolution (p<0.0001). ALDH1 + cells were 
      associated with mild dysplasia using a system from the World Health Organization 
      (WHO), and with a low risk of malignant transformation, according to the binary 
      system (p<0.0001). CD44+ cells were also associated with moderated dysplasia, 
      according to the WHO system. In LSCC, ALDH1 + cells were positively associated 
      with patients who were older (>/= 60 years old), smokers, and with those who 
      consumed alcohol (p<0.0001). CD44 + cells in LSCC were associated with older (>/= 
      60 years old) patients as well, but also with female patients, white skin, 
      non-smokers, and individuals who did not consume alcohol (p<0.0001), all of whom 
      showed distinct patterns in pre- and malignant lesions of both markers. 
      Additionally, in LSCC, both ALDH1 and CD44 staining were associated with smaller 
      tumor sizes (T1/T2; p<0.0001). In summary, although both ALDH1 and CD44 were 
      associated with the presence of dysplasia in AC lesions, the present findings 
      suggest that ALDH1 and CD44 may be activated by different etiopathogenic 
      pathways, predominantly in distinct steps of oral carcinogenesis. CD44 would thus 
      be more significantly related to the potentially malignant lesion, while ALDH1 
      would be closely linked to malignancy.
FAU - Ortiz, Rafael Carneiro
AU  - Ortiz RC
AUID- ORCID: 0000-0002-2794-0460
AD  - Universidade de Sao Paulo , Faculdade de Odontologia de Bauru , Departamento de 
      Ciencias Biologicas , Bauru , SP , Brasil .
FAU - Gois, Gabriele Gomes
AU  - Gois GG
AD  - Universidade de Sao Paulo , Faculdade de Odontologia de Bauru , Departamento de 
      Ciencias Biologicas , Bauru , SP , Brasil .
FAU - Costa, Camila Alves
AU  - Costa CA
AUID- ORCID: 0000-0002-8493-1835
AD  - Universidade Federal de Goias , Faculdade de Odontologia da Goiania , Centro de 
      Pesquisa em Saude Bucal Sistemica da Goias , Goiania , GO , Brasil .
FAU - Costa, Nadia Lago
AU  - Costa NL
AUID- ORCID: 0000-0003-0198-5828
AD  - Universidade Federal de Goias , Faculdade de Odontologia , Departamento de 
      Patologia Bucal, Estomatologia e Radiologia , Goiania , GO , Brasil .
FAU - Rodini, Camila Oliveira
AU  - Rodini CO
AUID- ORCID: 0000-0002-8763-4967
AD  - Universidade de Sao Paulo , Faculdade de Odontologia de Bauru , Departamento de 
      Ciencias Biologicas , Bauru , SP , Brasil .
LA  - eng
PT  - Journal Article
DEP - 20231222
PL  - Brazil
TA  - J Appl Oral Sci
JT  - Journal of applied oral science : revista FOB
JID - 101189774
RN  - EC 1.2.1 (Aldehyde Dehydrogenase 1 Family)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CD44 protein, human)
RN  - 0 (Hyaluronan Receptors)
RN  - Actinic cheilitis
SB  - IM
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Aldehyde Dehydrogenase 1 Family
MH  - Biomarkers, Tumor
MH  - Carcinogenesis
MH  - Hyaluronan Receptors/metabolism
MH  - Lip/metabolism/pathology
MH  - *Lip Neoplasms/etiology/metabolism/pathology
MH  - *Squamous Cell Carcinoma of Head and Neck/etiology/metabolism/pathology
PMC - PMC10786456
COIS- Conflict of Interest The authors declare no conflicts of interest.
EDAT- 2023/12/21 12:42
MHDA- 2023/12/22 06:43
PMCR- 2023/12/22
CRDT- 2023/12/21 08:05
PHST- 2023/07/03 00:00 [received]
PHST- 2023/11/06 00:00 [accepted]
PHST- 2023/12/22 06:43 [medline]
PHST- 2023/12/21 12:42 [pubmed]
PHST- 2023/12/21 08:05 [entrez]
PHST- 2023/12/22 00:00 [pmc-release]
AID - S1678-77572023000100464 [pii]
AID - 10.1590/1678-7757-2023-0227 [doi]
PST - epublish
SO  - J Appl Oral Sci. 2023 Dec 22;31:e20230227. doi: 10.1590/1678-7757-2023-0227. 
      eCollection 2023.