PMID- 38128501
OWN - NLM
STAT- Publisher
LR  - 20231221
IS  - 1423-0291 (Electronic)
IS  - 1015-2008 (Linking)
DP  - 2023 Dec 21
TI  - Molecular characterization and genetic subclassification comparison of diffuse 
      large B-cell lymphoma (DLBCL) - real-life experience with 74 cases.
LID - 10.1159/000535938 [doi]
AB  - INTRODUCTION: Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous entity. 
      Lately, several algorithms achieving therapeutically and prognostically relevant 
      DLBCL subclassification have been published. METHODS: A cohort of 74 routine 
      DLBCL cases was broadly characterized by immunohistochemistry (IHC), fluorescence 
      in situ hybridization (FISH) of the BCL2, BCL6 and MYC loci, and comprehensive 
      high throughput sequencing (HTS). Based on the genetic alterations found, cases 
      were reclassified using two probabilistic tools - LymphGen and Two-step 
      classifier, allowing for comparison of the two models. RESULTS: Hans and Tally's 
      overall IHC-based subclassification success rate was 96% and 82%, respectively. 
      HTS and FISH data allowed the LymphGen algorithm to successfully classify 11/55 
      cases, (1 - BN2, 7 - EZB, 1 - MCD, and 2 - genetically composite EZB/N1). The 
      total subclassification rate was 20%. On the other hand, the Two-step classifier 
      categorized 36/55 cases, with 65.5% success (9 - BN2, 12 - EZB, 9 - MCD, 2 - N1, 
      and 4 - ST2). Clinical correlations highlighted MCD as an aggressive subtype 
      associated with higher relapse and mortality. CONCLUSIONS: The Two-step algorithm 
      has a better success rate at subclassifying DLBCL cases based on genetic 
      differences. Further improvement of the classifiers is required to increase the 
      number of classifiable cases and thus prove their applicability in routine 
      diagnostics.
CI  - The Author(s). Published by S. Karger AG, Basel.
FAU - Ivanova, Vanesa-Sindi
AU  - Ivanova VS
FAU - Vela, Visar
AU  - Vela V
FAU - Dirnhofer, Stefan
AU  - Dirnhofer S
FAU - Dobbie, Michael
AU  - Dobbie M
FAU - Stenner, Frank
AU  - Stenner F
FAU - Knoblich, Jan
AU  - Knoblich J
FAU - Tzankov, Alexandar
AU  - Tzankov A
FAU - Menter, Thomas
AU  - Menter T
LA  - eng
PT  - Journal Article
DEP - 20231221
PL  - Switzerland
TA  - Pathobiology
JT  - Pathobiology : journal of immunopathology, molecular and cellular biology
JID - 9007504
SB  - IM
EDAT- 2023/12/22 00:42
MHDA- 2023/12/22 00:42
CRDT- 2023/12/21 18:27
PHST- 2023/09/21 00:00 [received]
PHST- 2023/12/18 00:00 [accepted]
PHST- 2023/12/22 00:42 [medline]
PHST- 2023/12/22 00:42 [pubmed]
PHST- 2023/12/21 18:27 [entrez]
AID - 000535938 [pii]
AID - 10.1159/000535938 [doi]
PST - aheadofprint
SO  - Pathobiology. 2023 Dec 21. doi: 10.1159/000535938.