PMID- 38132291
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231224
IS  - 2079-7737 (Print)
IS  - 2079-7737 (Electronic)
IS  - 2079-7737 (Linking)
VI  - 12
IP  - 12
DP  - 2023 Nov 24
TI  - Sja-Let-7 Attenuates Carbon Tetrachloride-Induced Liver Fibrosis in a Mouse Model 
      via Col1alpha2.
LID - 10.3390/biology12121465 [doi]
LID - 1465
AB  - Liver fibrosis (LF) is a chronic progressive disease with no definitive 
      treatment. The aim of this study was to assess helminth-derived molecules as 
      potential therapeutic targets to prevent or reverse LF. A mouse model of carbon 
      tetrachloride (CCL(4))-induced LF was established and sja-let-7 was overexpressed 
      by treatment with a miRNA agomir once per week. After four weeks, serum 
      biochemistry, hepatic hydroxyproline content measurements, liver histology, mRNA 
      expression profiling of fibrotic markers, the dual-luciferase reporter assay, and 
      fluorescence in situ hybridization (FISH) were performed. Administration of the 
      sja-let-7 agomir markedly ameliorated hepatosplenomegaly and reduced the liver 
      hydroxyproline content. Liver histological analysis showed significant reductions 
      in collagen deposition in the sja-let-7 agomir-treated mice. Additionally, the 
      mRNA levels of both pro-fibrotic markers and pro-inflammatory cytokines were 
      diminished after treatment. Furthermore, the dual-luciferase reporter assay and 
      FISH identified the alpha2 chain of collagen type 1 (Col1alpha2) as the direct target of 
      sja-let-7. Accordingly, the progression of LF was attenuated by targeting Col1alpha2 
      and the TGF-beta/Smad signaling pathway.
FAU - Zhong, Haoran
AU  - Zhong H
AUID- ORCID: 0000-0003-0967-9542
AD  - National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, 
      China.
AD  - Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural 
      Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Shanghai 200241, China.
FAU - Dong, Bowen
AU  - Dong B
AD  - National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, 
      China.
AD  - Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural 
      Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Shanghai 200241, China.
FAU - Zhu, Danlin
AU  - Zhu D
AD  - National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, 
      China.
AD  - Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural 
      Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Shanghai 200241, China.
FAU - Li, Hao
AU  - Li H
AD  - National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, 
      China.
AD  - Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural 
      Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Shanghai 200241, China.
FAU - Lu, Ke
AU  - Lu K
AD  - National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, 
      China.
AD  - Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural 
      Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Shanghai 200241, China.
FAU - Fu, Zhiqiang
AU  - Fu Z
AD  - National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, 
      China.
AD  - Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural 
      Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Shanghai 200241, China.
FAU - Liu, Jinming
AU  - Liu J
AD  - National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, 
      China.
AD  - Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural 
      Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Shanghai 200241, China.
FAU - Jin, Yamei
AU  - Jin Y
AD  - National Reference Laboratory for Animal Schistosomiasis, Shanghai Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, 
      China.
AD  - Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural 
      Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural 
      Sciences, Shanghai 200241, China.
LA  - eng
GR  - 20ZR1469300/Natural Science Foundation of Shanghai/
GR  - 31672245/Chinese National Natural Science Foundation/
PT  - Journal Article
DEP - 20231124
PL  - Switzerland
TA  - Biology (Basel)
JT  - Biology
JID - 101587988
PMC - PMC10740823
OTO - NOTNLM
OT  - Col1alpha2
OT  - TGF-beta/Smad signaling pathway
OT  - hepatic stellate cell
OT  - liver fibrosis
OT  - sja-let-7
COIS- The authors declare no conflict of interest.
EDAT- 2023/12/22 12:42
MHDA- 2023/12/22 12:43
PMCR- 2023/11/24
CRDT- 2023/12/22 09:04
PHST- 2023/10/17 00:00 [received]
PHST- 2023/11/07 00:00 [revised]
PHST- 2023/11/17 00:00 [accepted]
PHST- 2023/12/22 12:43 [medline]
PHST- 2023/12/22 12:42 [pubmed]
PHST- 2023/12/22 09:04 [entrez]
PHST- 2023/11/24 00:00 [pmc-release]
AID - biology12121465 [pii]
AID - biology-12-01465 [pii]
AID - 10.3390/biology12121465 [doi]
PST - epublish
SO  - Biology (Basel). 2023 Nov 24;12(12):1465. doi: 10.3390/biology12121465.