PMID- 38132483
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231224
IS  - 1467-3045 (Electronic)
IS  - 1467-3037 (Print)
IS  - 1467-3037 (Linking)
VI  - 45
IP  - 12
DP  - 2023 Dec 18
TI  - Water Extract of Portulaca Oleracea Inhibits PEDV Infection-Induced Pyrolysis by 
      Caspase-1/GSDMD.
PG  - 10211-10224
LID - 10.3390/cimb45120637 [doi]
AB  - Porcine epidemic diarrhea virus (PEDV) belongs to the coronavirus family and the 
      coronavirus genus, causing contact enteric infection in pigs. It is one of the 
      most serious diseases that threatens the pig industry. However, there is 
      currently no specific drug to prevent and treat the disease, indicating that we 
      need to be vigilant about the spread of the disease and the development of 
      anti-PEDV drugs. The dried aerial parts of the plant Portulaca oleracea in the 
      family Portulacaceous, whose decoction can be used to treat acute enteritis, 
      dysentery, diarrhea, and other diseases. This study explored the potential 
      mechanism of water extract of Portulaca oleracea (WEPO) in PEDV-induced 
      pyroptosis in Vero cells. PEDV decreased the viability of Vero cells in a dose- 
      and time-dependent manner, causing cell damage, upregulating the level of 
      intracellular Nlrp3, and inhibiting the level of Gasdermin D (GSDMD) and the 
      activation of Caspase-1. WEPO can inhibit PEDV-induced pyroptosis, reduce the 
      elevation of inflammatory factors caused by infection, and exhibit a 
      dose-dependent effect. Knockdown of Caspase-1 and GSDMD separately can induce the 
      production of the inflammatory factor IL-1beta to significantly decrease and 
      increase, respectively. These results suggest that WEPO can inhibit cell 
      pyroptosis caused by PEDV and that the Caspase-1 and GSDMD pathways play an 
      important role in this process.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - College of Veterinary Medicine, Qingdao Agricultural University, No. 700 
      Changcheng Road, Qingdao 266109, China.
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong 
      Provincial Animal and Poultry Green Health Products Creation Engineering 
      Laboratory, Jinan 250100, China.
FAU - Liu, Yueyue
AU  - Liu Y
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong 
      Provincial Animal and Poultry Green Health Products Creation Engineering 
      Laboratory, Jinan 250100, China.
FAU - Yang, Shifa
AU  - Yang S
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong 
      Provincial Animal and Poultry Green Health Products Creation Engineering 
      Laboratory, Jinan 250100, China.
FAU - Yin, Bin
AU  - Yin B
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong 
      Provincial Animal and Poultry Green Health Products Creation Engineering 
      Laboratory, Jinan 250100, China.
FAU - Zhao, Zengcheng
AU  - Zhao Z
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong 
      Provincial Animal and Poultry Green Health Products Creation Engineering 
      Laboratory, Jinan 250100, China.
FAU - Huang, Zhongli
AU  - Huang Z
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong 
      Provincial Animal and Poultry Green Health Products Creation Engineering 
      Laboratory, Jinan 250100, China.
FAU - Wu, Jiaqiang
AU  - Wu J
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong 
      Provincial Animal and Poultry Green Health Products Creation Engineering 
      Laboratory, Jinan 250100, China.
AD  - Key Laboratory of Animal Resistant Biology of Shandong, College of Life Sciences, 
      Shandong Normal University, Jinan 250014, China.
FAU - Lin, Shuqian
AU  - Lin S
AD  - Institute of Poultry Science, Shandong Academy of Agricultural Science, Shandong 
      Provincial Animal and Poultry Green Health Products Creation Engineering 
      Laboratory, Jinan 250100, China.
FAU - Wang, Xin
AU  - Wang X
AD  - College of Veterinary Medicine, Qingdao Agricultural University, No. 700 
      Changcheng Road, Qingdao 266109, China.
LA  - eng
GR  - 32302918/National Natural Science Funds/
GR  - 2020CXGC010801/Major Science and Technology Innovation Project of Shandong 
      Province/
GR  - 2023TSGC0006/Innovation Ca-pacity Improvement Project for Technology-based SMEs 
      in Shandong Province/
GR  - 2022/Shandong Provincial Science and Technology Achievement Transfer and 
      Transformation Subsidy (Shandong-Chongqing Cooperation) Project/
PT  - Journal Article
DEP - 20231218
PL  - Switzerland
TA  - Curr Issues Mol Biol
JT  - Current issues in molecular biology
JID - 100931761
PMC - PMC10742930
OTO - NOTNLM
OT  - PEDV
OT  - Portulaca oleracea
OT  - caspase-1/GSDMD
OT  - in-vitro analysis
OT  - pyrolysis
COIS- The authors declare no conflict of interest.
EDAT- 2023/12/22 12:43
MHDA- 2023/12/22 12:44
PMCR- 2023/12/18
CRDT- 2023/12/22 09:07
PHST- 2023/11/16 00:00 [received]
PHST- 2023/12/07 00:00 [revised]
PHST- 2023/12/11 00:00 [accepted]
PHST- 2023/12/22 12:44 [medline]
PHST- 2023/12/22 12:43 [pubmed]
PHST- 2023/12/22 09:07 [entrez]
PHST- 2023/12/18 00:00 [pmc-release]
AID - cimb45120637 [pii]
AID - cimb-45-00637 [pii]
AID - 10.3390/cimb45120637 [doi]
PST - epublish
SO  - Curr Issues Mol Biol. 2023 Dec 18;45(12):10211-10224. doi: 10.3390/cimb45120637.