PMID- 38134557
OWN - NLM
STAT- MEDLINE
DCOM- 20240101
LR  - 20240408
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 174
DP  - 2024 Feb
TI  - Anti-inflammatory effects of neuregulin-1 in HaCaT keratinocytes and atopic 
      dermatitis-like mice stimulated with Der p 38.
PG  - 156439
LID - S1043-4666(23)00317-4 [pii]
LID - 10.1016/j.cyto.2023.156439 [doi]
AB  - Neuregulin (NRG)-1 plays fundamental roles in several organ systems after binding 
      to its receptors, ErbB2 and ErbB4. This study examines the role of NRG-1 in 
      atopic dermatitis (AD), a chronic skin disease that causes dryness, pruritus, and 
      inflammation. In mice administered Der p 38, the skin presents AD-like symptoms 
      including filaggrin downregulation and infiltration of neutrophils and 
      eosinophils. Noticeably, there is an increased expression of NRG-1, ErbB2, and 
      ErbB4 in the skin. Upregulation of these proteins is significantly correlated to 
      the clinical skin severity score. In human keratinocyte HaCaT cells, exposure to 
      Der p 38 decreased filaggrin expression, and NRG-1 alone had no effect on the 
      expression. However, co-treatment of Der p 38 with NRG-1 enhanced the filaggrin 
      expression decreased by Der p 38. Pre-treatment with AG879 (an ErbB2 inhibitor) 
      or ErbB4 siRNA blocked the recovery of filaggrin expression in the cells after 
      co-treatment with Der p 38 and NRG-1. Der p 38 treatment enhanced the secretion 
      of interleukin-6 (IL-6), IL-8, and monocyte chemoattractant protein-1 (MCP-1). 
      Co-treatment of Der p 38 with NRG-1 lowered the cytokine secretion increased by 
      Der p 38, although NRG-1 alone was not effective on cytokine alteration. 
      Neutrophil apoptosis was not altered by NRG-1 or supernatants of cells treated 
      with NRG-1, but the cell supernatants co-treated with Der p 38 and NRG-1 blocked 
      the anti-apoptotic effects of Der p 38-treated supernatants on neutrophils, which 
      was involved in the activation of caspase 9 and caspase 3. Taken together, we 
      determined that NRG-1 has anti-inflammatory effects in AD triggered by Der p 38. 
      These results will pave the way to understanding the functions of NRG-1 and in 
      the future development of AD treatment.
CI  - Copyright (c) 2023. Published by Elsevier Ltd.
FAU - Yun, Jeong Hee
AU  - Yun JH
AD  - Department of Biomedical Laboratory Science, College of Health Science, Eulji 
      University, Uijeongbu 11759, Republic of Korea.
FAU - Hong, Yujin
AU  - Hong Y
AD  - Department of Senior Healthcare, Graduate School, Eulji University, Uijeongbu 
      11759, Republic of Korea.
FAU - Hong, Min Hwa
AU  - Hong MH
AD  - Department of Senior Healthcare, Graduate School, Eulji University, Uijeongbu 
      11759, Republic of Korea.
FAU - Kim, Geunyeong
AU  - Kim G
AD  - Department of Senior Healthcare, Graduate School, Eulji University, Uijeongbu 
      11759, Republic of Korea.
FAU - Lee, Ji-Sook
AU  - Lee JS
AD  - Department of Clinical Laboratory Science, Wonkwang Health Science University, 
      Iksan 54538, Republic of Korea.
FAU - Woo, Ran-Sook
AU  - Woo RS
AD  - Department of Anatomy and Neuroscience, Eulji University School of Medicine, 
      Daejeon 34824, Republic of Korea.
FAU - Lee, Juram
AU  - Lee J
AD  - Department of Biomedical Laboratory Science, College of Health Science, Eulji 
      University, Uijeongbu 11759, Republic of Korea.
FAU - Yang, Eun Ju
AU  - Yang EJ
AD  - Department of Biomedical Laboratory Science, Daegu Haany University, Gyeongsan 
      38610, Republic of Korea. Electronic address: ejyang@dhu.ac.kr.
FAU - Kim, In Sik
AU  - Kim IS
AD  - Department of Biomedical Laboratory Science, College of Health Science, Eulji 
      University, Uijeongbu 11759, Republic of Korea; Department of Senior Healthcare, 
      Graduate School, Eulji University, Uijeongbu 11759, Republic of Korea. Electronic 
      address: orientree@eulji.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20231221
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Filaggrin Proteins)
RN  - 0 (Neuregulin-1)
RN  - 0 (Der p 38)
RN  - 0 (Cytokines)
RN  - EC 2.7.10.1 (Receptor, ErbB-4)
RN  - 0 (Anti-Inflammatory Agents)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Humans
MH  - *Dermatitis, Atopic/genetics
MH  - Filaggrin Proteins
MH  - Neuregulin-1/pharmacology/metabolism/therapeutic use
MH  - Keratinocytes/metabolism
MH  - Skin/metabolism
MH  - Cytokines/metabolism
MH  - Receptor, ErbB-4/metabolism/pharmacology
MH  - Anti-Inflammatory Agents/pharmacology
OTO - NOTNLM
OT  - Anti-inflammatory effect
OT  - Atopic dermatitis
OT  - Cytokine secretion
OT  - NRG-1
OT  - Neutrophil apoptosis
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/12/22 19:43
MHDA- 2024/01/02 11:44
CRDT- 2023/12/22 18:10
PHST- 2023/07/25 00:00 [received]
PHST- 2023/11/07 00:00 [revised]
PHST- 2023/11/10 00:00 [accepted]
PHST- 2024/01/02 11:44 [medline]
PHST- 2023/12/22 19:43 [pubmed]
PHST- 2023/12/22 18:10 [entrez]
AID - S1043-4666(23)00317-4 [pii]
AID - 10.1016/j.cyto.2023.156439 [doi]
PST - ppublish
SO  - Cytokine. 2024 Feb;174:156439. doi: 10.1016/j.cyto.2023.156439. Epub 2023 Dec 21.