PMID- 38134667
OWN - NLM
STAT- MEDLINE
DCOM- 20240112
LR  - 20240112
IS  - 1095-7103 (Electronic)
IS  - 0021-9797 (Linking)
VI  - 658
DP  - 2024 Mar 15
TI  - Size and phase preservation of amorphous calcium carbonate nanoparticles in 
      aqueous media using different types of lignin for contrast-enhanced ultrasound 
      imaging.
PG  - 584-596
LID - S0021-9797(23)02427-X [pii]
LID - 10.1016/j.jcis.2023.12.106 [doi]
AB  - HYPOTHESIS: Calcium carbonate (CaCO(3)) nanoparticles could have great potential 
      for contrast-enhanced ultrasound imaging (CEUS) due to their gas-generating 
      properties and sensitivity to physiological conditions. However, the use of nano 
      CaCO(3) for biomedical applications requires the assistance of stabilizers to 
      control the size and avoid the fast dissolution/recrystallization of the 
      particles when exposed to aqueous conditions. EXPERIMENTS: Herein, we report the 
      stabilization of nano CaCO(3) using lignin, and synthesized core-shell amorphous 
      CaCO(3)-lignin nanoparticles (LigCC NPs) with a diameter below 100 nm. We have 
      then investigated the echogenicity of the LigCC NPs by monitoring the consequent 
      generation of contrast in vitro for 90 min in linear and non-linear B-mode 
      imaging. FINDINGS: This research explores how lignin type and structure affect 
      stabilization efficiency, lignin structuration around CaCO(3) cores, and particle 
      echogenicity. Interestingly, by employing lignin as the stabilizer, it becomes 
      possible to maintain the echogenic properties of CaCO(3), whereas the use of 
      lipid coatings prevents the production of signal generation in ultrasound 
      imaging. This work opens new avenue for CEUS imaging of the vascular and 
      extravascular space using CaCO(3), as it highlights the potential to generate 
      contrast for extended durations at physiological pH by utilizing the amorphous 
      phase of CaCO(3).
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Chuzeville, Lauriane
AU  - Chuzeville L
AD  - Materials Research and Technology (MRT), Luxembourg Institute of Science and 
      Technology, 5 Avenue des Hauts Fourneaux, Esch/Alzette L-4362, Luxembourg; 
      University of Luxembourg, Department of Physics & Materials Science, 162a Avenue 
      de la Faiencerie, 1511 Luxembourg city, Luxembourg.
FAU - Aissani, Abderrahmane
AU  - Aissani A
AD  - Sorbonne Universite, CNRS, INSERM, Laboratoire d'Imagerie Biomedicale, 15 rue de 
      l'ecole de medecine, 75006 Paris, France.
FAU - Manisekaran, Ahilan
AU  - Manisekaran A
AD  - Materials Research and Technology (MRT), Luxembourg Institute of Science and 
      Technology, 5 Avenue des Hauts Fourneaux, Esch/Alzette L-4362, Luxembourg; 
      University of Luxembourg, Department of Physics & Materials Science, 162a Avenue 
      de la Faiencerie, 1511 Luxembourg city, Luxembourg.
FAU - Fleming, Yves
AU  - Fleming Y
AD  - Materials Research and Technology (MRT), Luxembourg Institute of Science and 
      Technology, 5 Avenue des Hauts Fourneaux, Esch/Alzette L-4362, Luxembourg.
FAU - Grysan, Patrick
AU  - Grysan P
AD  - Materials Research and Technology (MRT), Luxembourg Institute of Science and 
      Technology, 5 Avenue des Hauts Fourneaux, Esch/Alzette L-4362, Luxembourg.
FAU - Contal, Servane
AU  - Contal S
AD  - Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of 
      Science and Technology, 5 Avenue des Hauts Fourneaux, Esch/Alzette L-4362, 
      Luxembourg.
FAU - Chary, Aline
AU  - Chary A
AD  - Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of 
      Science and Technology, 5 Avenue des Hauts Fourneaux, Esch/Alzette L-4362, 
      Luxembourg.
FAU - Duday, David
AU  - Duday D
AD  - Materials Research and Technology (MRT), Luxembourg Institute of Science and 
      Technology, 5 Avenue des Hauts Fourneaux, Esch/Alzette L-4362, Luxembourg.
FAU - Couture, Olivier
AU  - Couture O
AD  - Sorbonne Universite, CNRS, INSERM, Laboratoire d'Imagerie Biomedicale, 15 rue de 
      l'ecole de medecine, 75006 Paris, France.
FAU - Anand, Resmi
AU  - Anand R
AD  - Materials Research and Technology (MRT), Luxembourg Institute of Science and 
      Technology, 5 Avenue des Hauts Fourneaux, Esch/Alzette L-4362, Luxembourg.
FAU - Thomann, Jean-Sebastien
AU  - Thomann JS
AD  - Materials Research and Technology (MRT), Luxembourg Institute of Science and 
      Technology, 5 Avenue des Hauts Fourneaux, Esch/Alzette L-4362, Luxembourg. 
      Electronic address: jean-sebastien.thomann@list.lu.
LA  - eng
PT  - Journal Article
DEP - 20231218
PL  - United States
TA  - J Colloid Interface Sci
JT  - Journal of colloid and interface science
JID - 0043125
RN  - 9005-53-2 (Lignin)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - *Lignin
MH  - *Nanoparticles/chemistry
MH  - Ultrasonography/methods
MH  - Calcium Carbonate/chemistry
MH  - Water
OTO - NOTNLM
OT  - Calcium carbonate nanoparticles
OT  - Contrast-enhanced ultrasound imaging
OT  - Core-shell particles
OT  - Lignin
COIS- Declaration of competing interest The authors declare the following financial 
      interests/personal relationships which may be considered as potential competing 
      interests: J-S. Thomann has patent # LU102358B1 ; WO2022144136A1 pending to 
      Luxembourg Institute of Science and Technology (LIST). L. Chuzeville has patent # 
      LU102358B1;WO2022144136A1 pending to Luxembourg Institute of Science and 
      Technology (LIST).
EDAT- 2023/12/22 19:43
MHDA- 2024/01/12 06:43
CRDT- 2023/12/22 18:16
PHST- 2023/09/14 00:00 [received]
PHST- 2023/12/04 00:00 [revised]
PHST- 2023/12/17 00:00 [accepted]
PHST- 2024/01/12 06:43 [medline]
PHST- 2023/12/22 19:43 [pubmed]
PHST- 2023/12/22 18:16 [entrez]
AID - S0021-9797(23)02427-X [pii]
AID - 10.1016/j.jcis.2023.12.106 [doi]
PST - ppublish
SO  - J Colloid Interface Sci. 2024 Mar 15;658:584-596. doi: 
      10.1016/j.jcis.2023.12.106. Epub 2023 Dec 18.