PMID- 38138514
OWN - NLM
STAT- MEDLINE
DCOM- 20231225
LR  - 20240102
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 28
IP  - 24
DP  - 2023 Dec 9
TI  - Identification of a beta-Carboline Alkaloid from Chemoselectively Derived Vanilla 
      Bean Extract and Its Prevention of Lipid Droplet Accumulation in Human 
      Hepatocytes (HepG2).
LID - 10.3390/molecules28248024 [doi]
LID - 8024
AB  - Targeting bioactive compounds to prevent lipid droplet accumulation in the liver, 
      we explored an antioxidative extract from vanilla bean (Vainilla planifolia) 
      after chemo-selective derivatization through heating and acid modification. The 
      chemical analysis of vanilla bean extract through chemoselective derivatization 
      resulted in the identification of sixteen compounds (34-50) using LC-MS/MS 
      analysis. A beta-carboline alkaloid with a piperidine C-ring and a vanillin moiety 
      at C-1 (34) was identified by molecular networking and diagnostic fragmentation 
      filtering approaches. beta-carboline alkaloid 34 exhibited significant inhibitory 
      activity of lipid droplet accumulation (LDAI) in oleic acid-loaded hepatocellular 
      carcinoma HepG2 cells. The LDAI activity was associated with both activation of 
      lipolysis and suppression of lipogenesis in the cells. The study indicates that 
      crude plant extracts, following chemoselective derivatization, may contain 
      bioactive compounds that could be beneficial in preventing hepatosteatosis and 
      could serve as a source of lead compounds for drug development. This approach may 
      be useful to investigate other mixtures of natural products and food resources.
FAU - Dibwe, Dya Fita
AU  - Dibwe DF
AUID- ORCID: 0000-0003-3433-3834
AD  - Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-Ku, 
      Sapporo 060-0812, Japan.
FAU - Takeishi, Nire
AU  - Takeishi N
AD  - Graduate School of Health Sciences, Hokkaido University, Kita-12, Nishi-5, 
      Kita-Ku, Sapporo 060-0812, Japan.
FAU - Oba, Saki
AU  - Oba S
AD  - Graduate School of Health Sciences, Hokkaido University, Kita-12, Nishi-5, 
      Kita-Ku, Sapporo 060-0812, Japan.
FAU - Sakurai, Akiko
AU  - Sakurai A
AD  - Graduate School of Health Sciences, Hokkaido University, Kita-12, Nishi-5, 
      Kita-Ku, Sapporo 060-0812, Japan.
FAU - Sakurai, Toshihiro
AU  - Sakurai T
AUID- ORCID: 0000-0003-1759-3802
AD  - Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-Ku, 
      Sapporo 060-0812, Japan.
FAU - Tsukui, Takayuki
AU  - Tsukui T
AUID- ORCID: 0000-0002-9150-5199
AD  - Department of Nutrition, Sapporo University of Health Sciences, Nakanuma 
      Nishi-4-3-1-15, Higashi-Ku, Sapporo 007-0894, Japan.
FAU - Chiba, Hitoshi
AU  - Chiba H
AUID- ORCID: 0000-0003-4640-7730
AD  - Department of Nutrition, Sapporo University of Health Sciences, Nakanuma 
      Nishi-4-3-1-15, Higashi-Ku, Sapporo 007-0894, Japan.
FAU - Hui, Shu-Ping
AU  - Hui SP
AUID- ORCID: 0000-0001-9973-6461
AD  - Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-Ku, 
      Sapporo 060-0812, Japan.
LA  - eng
PT  - Journal Article
DEP - 20231209
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Alkaloids)
RN  - 0 (Plant Extracts)
RN  - 0 (Carbolines)
SB  - IM
MH  - Humans
MH  - *Vanilla/chemistry
MH  - Chromatography, Liquid
MH  - Lipid Droplets
MH  - Tandem Mass Spectrometry
MH  - *Alkaloids/pharmacology
MH  - Plant Extracts/pharmacology/chemistry
MH  - Hep G2 Cells
MH  - Carbolines/pharmacology
PMC - PMC10745329
OTO - NOTNLM
OT  - Orbitrap LC/MS
OT  - bean extracts
OT  - bioactive alkaloids
OT  - functional foods
OT  - lipid droplet accumulation inhibition
OT  - lipidomics
OT  - neutral lipids
OT  - triacylglycerols
COIS- The authors declare no conflict of interest.
EDAT- 2023/12/23 12:43
MHDA- 2023/12/25 06:43
PMCR- 2023/12/09
CRDT- 2023/12/23 01:16
PHST- 2023/09/12 00:00 [received]
PHST- 2023/12/02 00:00 [revised]
PHST- 2023/12/03 00:00 [accepted]
PHST- 2023/12/25 06:43 [medline]
PHST- 2023/12/23 12:43 [pubmed]
PHST- 2023/12/23 01:16 [entrez]
PHST- 2023/12/09 00:00 [pmc-release]
AID - molecules28248024 [pii]
AID - molecules-28-08024 [pii]
AID - 10.3390/molecules28248024 [doi]
PST - epublish
SO  - Molecules. 2023 Dec 9;28(24):8024. doi: 10.3390/molecules28248024.