PMID- 38138918
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231225
IS  - 2075-4426 (Print)
IS  - 2075-4426 (Electronic)
IS  - 2075-4426 (Linking)
VI  - 13
IP  - 12
DP  - 2023 Dec 5
TI  - Blood Plasma Circulating DNA-Protein Complexes: Involvement in Carcinogenesis and 
      Prospects for Liquid Biopsy of Breast Cancer.
LID - 10.3390/jpm13121691 [doi]
LID - 1691
AB  - Circulating DNA (cirDNA) is a promising tool in translational medicine. However, 
      studies of cirDNA have neglected its association with proteins, despite ample 
      evidence that this interaction may affect the fate of DNA in the bloodstream and 
      its molecular functions. The goal of the current study is to shed light on the 
      differences between the proteomic cargos of histone-containing nucleoprotein 
      complexes (NPCs) from healthy female (HFs) and breast cancer patients (BCPs), and 
      to reveal the proteins involved in carcinogenesis. NPCs were isolated from the 
      blood samples of HFs and BCPs using affinity chromatography. A total of 177 and 
      169 proteins were identified in NPCs from HFs and BCPs using MALDI-TOF mass 
      spectrometry. A bioinformatics analysis revealed that catalytically active 
      proteins, as well as proteins that bind nucleic acids and regulate the activity 
      of receptors, are the most represented among the unique proteins of blood NPCs 
      from HFs and BCPs. In addition, the proportion of proteins participating in ion 
      channels and proteins binding proteins increases in the NPCs from BCP blood. 
      However, the involvement in transport and signal transduction was greater in BCP 
      NPCs compared to those from HFs. Gene ontology term (GO) analysis revealed that 
      the NPC protein cargo from HF blood was enriched with proteins involved in the 
      negative regulation of cell proliferation, and in BCP blood, proteins involved in 
      EMT, invasion, and cell migration were observed. The combination of SPG7, ADRB1, 
      SMCO4, PHF1, and PSMG1 NPC proteins differentiates BCPs from HFs with a 
      sensitivity of 100% and a specificity of 80%. The obtained results indirectly 
      indicate that, in tandem with proteins, blood cirDNA is an important part of 
      intercellular communication, playing a regulatory and integrating role in the 
      physiology of the body.
FAU - Shefer, Aleksei
AU  - Shefer A
AUID- ORCID: 0000-0001-5369-397X
AD  - V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 
      630090 Novosibirsk, Russia.
FAU - Tutanov, Oleg
AU  - Tutanov O
AD  - Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 
      37203, USA.
FAU - Belenikin, Maxim
AU  - Belenikin M
AD  - Evogen LLC, 115191 Moscow, Russia.
FAU - Tsentalovich, Yuri P
AU  - Tsentalovich YP
AUID- ORCID: 0000-0002-1380-3000
AD  - International Tomography Center, Siberian Branch of Russian Academy of Sciences, 
      630090 Novosibirsk, Russia.
FAU - Tamkovich, Svetlana
AU  - Tamkovich S
AUID- ORCID: 0000-0001-7774-943X
AD  - V. Zelman Institute for Medicine and Psychology, Novosibirsk State University, 
      630090 Novosibirsk, Russia.
LA  - eng
GR  - 22-25-00130/Russian Science Foundation/
PT  - Journal Article
DEP - 20231205
PL  - Switzerland
TA  - J Pers Med
JT  - Journal of personalized medicine
JID - 101602269
PMC - PMC10744380
OTO - NOTNLM
OT  - MALDI-TOF mass spectrometry
OT  - bioinformatics analysis
OT  - breast cancer
OT  - circulating DNA
OT  - liquid biopsy
OT  - nucleoprotein complexes
OT  - plasma
COIS- Dr. Maxim Belenikin is an employee of Evogen LLC. He was engaged in analyzing 
      proteins on Gene ontology term (GO). This analytical work is in no way related to 
      the company he works for.
EDAT- 2023/12/23 12:43
MHDA- 2023/12/23 12:44
PMCR- 2023/12/05
CRDT- 2023/12/23 01:19
PHST- 2023/10/12 00:00 [received]
PHST- 2023/11/21 00:00 [revised]
PHST- 2023/11/29 00:00 [accepted]
PHST- 2023/12/23 12:44 [medline]
PHST- 2023/12/23 12:43 [pubmed]
PHST- 2023/12/23 01:19 [entrez]
PHST- 2023/12/05 00:00 [pmc-release]
AID - jpm13121691 [pii]
AID - jpm-13-01691 [pii]
AID - 10.3390/jpm13121691 [doi]
PST - epublish
SO  - J Pers Med. 2023 Dec 5;13(12):1691. doi: 10.3390/jpm13121691.