PMID- 38139040
OWN - NLM
STAT- MEDLINE
DCOM- 20231225
LR  - 20231225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 24
DP  - 2023 Dec 7
TI  - Metabolomics (Non-Targeted) of Induced Type 2 Diabetic Sprague Dawley Rats 
      Comorbid with a Tissue-Dwelling Nematode Parasite.
LID - 10.3390/ijms242417211 [doi]
LID - 17211
AB  - Type 2 diabetes is a non-communicable metabolic syndrome that is characterized by 
      the dysfunction of pancreatic beta-cells and insulin resistance. Both animal and 
      human studies have been conducted, demonstrating that helminth infections are 
      associated with a decreased prevalence of type 2 diabetes mellitus (T2DM). 
      However, there is a paucity of information on the impact that helminths have on 
      the metabolome of the host and how the infection ameliorates T2DM or its 
      progression. Therefore, this study aimed at using a non-targeted metabolomics 
      approach to systematically identify differentiating metabolites from serum 
      samples of T2DM-induced Sprague Dawley (SD) rats infected with a tissue-dwelling 
      nematode, Trichinella zimbabwensis, and determine the metabolic pathways impacted 
      during comorbidity. Forty-five male SD rats with a body weight between 160 g and 
      180 g were used, and these were randomly selected into control (non-diabetic and 
      not infected with T. zimbabwensis) (n = 15) and T2DM rats infected with T. 
      zimbabwensis (TzDM) (n = 30). The results showed metabolic separation between the 
      two groups, where d-mannitol, d-fructose, and glucose were upregulated in the 
      TzDM group, when compared to the control group. L-tyrosine, glycine, diglycerol, 
      L-lysine, and L-hydroxyproline were downregulated in the TzDM group when compared 
      to the control group. Metabolic pathways which were highly impacted in the TzDM 
      group include biotin metabolism, carnitine synthesis, and lactose degradation. We 
      conclude from our study that infecting T2DM rats with a tissue-dwelling nematode, 
      T. zimbabwensis, causes a shift in the metabolome, causing changes in different 
      metabolic pathways. Additionally, the infection showed the potential to regulate 
      or improve diabetes complications by causing a decrease in the amino acid 
      concentration that results in metabolic syndrome.
FAU - Ndlovu, Innocent Siyanda
AU  - Ndlovu IS
AUID- ORCID: 0000-0002-1384-2026
AD  - School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 
      4001, South Africa.
FAU - Tshilwane, Selaelo Ivy
AU  - Tshilwane SI
AUID- ORCID: 0000-0002-3549-4675
AD  - Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, 
      University of Pretoria, Pretoria 0110, South Africa.
FAU - Ngcamphalala, Philile Ignecious
AU  - Ngcamphalala PI
AD  - School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 
      4001, South Africa.
FAU - Vosloo, Andre'
AU  - Vosloo A
AD  - School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 
      4001, South Africa.
FAU - Chaisi, Mamohale
AU  - Chaisi M
AD  - Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, 
      University of Pretoria, Pretoria 0110, South Africa.
AD  - Foundational Biodiversity Science, South African National Biodiversity Institute, 
      Pretoria 0001, South Africa.
FAU - Mukaratirwa, Samson
AU  - Mukaratirwa S
AUID- ORCID: 0000-0001-6843-1497
AD  - School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 
      4001, South Africa.
AD  - One Health Center for Zoonoses and Tropical Veterinary Medicine, School of 
      Veterinary Medicine, Ross University, Basseterre KN0101, Saint Kitts and Nevis.
LA  - eng
GR  - 41011-20/Ross University School of Veterinary Medicine/
GR  - P2020/11/National Research Foundation: Professional Development Programme (PDP)/
PT  - Journal Article
DEP - 20231207
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Rats
MH  - Male
MH  - Humans
MH  - Animals
MH  - Rats, Sprague-Dawley
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - *Parasites
MH  - *Metabolic Syndrome
MH  - Metabolomics
MH  - *Trichinella
MH  - Comorbidity
PMC - PMC10743009
OTO - NOTNLM
OT  - Sprague Dawley rats
OT  - Trichinella zimbabwensis
OT  - amino acids
OT  - carbohydrates
OT  - metabolomics
OT  - type 2 diabetes
COIS- The authors declare no conflict of interest.
EDAT- 2023/12/23 12:43
MHDA- 2023/12/25 06:42
PMCR- 2023/12/07
CRDT- 2023/12/23 01:19
PHST- 2023/10/12 00:00 [received]
PHST- 2023/11/22 00:00 [revised]
PHST- 2023/11/27 00:00 [accepted]
PHST- 2023/12/25 06:42 [medline]
PHST- 2023/12/23 12:43 [pubmed]
PHST- 2023/12/23 01:19 [entrez]
PHST- 2023/12/07 00:00 [pmc-release]
AID - ijms242417211 [pii]
AID - ijms-24-17211 [pii]
AID - 10.3390/ijms242417211 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Dec 7;24(24):17211. doi: 10.3390/ijms242417211.