PMID- 38140601
OWN - NLM
STAT- MEDLINE
DCOM- 20231225
LR  - 20231225
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 15
IP  - 12
DP  - 2023 Nov 30
TI  - The Antiviral Effect of Berdazimer Sodium on Molluscum Contagiosum Virus Using a 
      Novel In Vitro Methodology.
LID - 10.3390/v15122360 [doi]
LID - 2360
AB  - Molluscum contagiosum (MC) is characterized by skin lesions containing the highly 
      contagious molluscum contagiosum poxvirus (MCV). MCV primarily infects children, 
      with one US Food and Drug Administration (FDA)-approved drug-device treatment in 
      use but no approved medications. Assessing antivirals is hindered by the 
      inability of MCV to replicate in vitro. Here, we use vaccinia virus as a 
      surrogate to provide evidence of the anti-poxvirus properties of berdazimer 
      sodium, a new chemical entity, and the active substance in berdazimer gel, 10.3%, 
      a nitric oxide-releasing topical in phase 3 development for the treatment of MC. 
      We show that berdazimer sodium reduced poxvirus replication and, through a novel 
      methodology, demonstrate that cells infected with drug-treated MCV virions have 
      reduced early gene expression. Specifically, this is accomplished by studying the 
      nuclear factor kappa-light-chain-enhancer of activated B cell (NF-kB)-blocking 
      protein MC160 as an example of an early gene. The results provide a plausible 
      unique antiviral mechanism of action supporting increased MCV resolution observed 
      in patients treated with berdazimer gel, 10.3% and describe a novel methodology 
      that overcomes limitations in investigating MCV response in vitro to a potential 
      new MC topical medication.
FAU - Ward, Brian M
AU  - Ward BM
AD  - Department of Microbiology and Immunology, University of Rochester Medical 
      Center, Rochester, NY 14642, USA.
FAU - Riccio, Daniel A
AU  - Riccio DA
AUID- ORCID: 0009-0001-4255-4306
AD  - Novan, Durham, NC 27703, USA.
FAU - Cartwright, Martina
AU  - Cartwright M
AUID- ORCID: 0000-0001-7207-2810
AD  - Novan, Durham, NC 27703, USA.
FAU - Maeda-Chubachi, Tomoko
AU  - Maeda-Chubachi T
AUID- ORCID: 0000-0002-3614-1505
AD  - Novan, Durham, NC 27703, USA.
LA  - eng
PT  - Journal Article
DEP - 20231130
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - ORT9SID4QY (berdazimer sodium)
RN  - 0 (Siloxanes)
RN  - 0 (Antiviral Agents)
SB  - IM
MH  - United States
MH  - Child
MH  - Humans
MH  - *Molluscum contagiosum virus/genetics
MH  - *Molluscum Contagiosum/drug therapy
MH  - Siloxanes/metabolism
MH  - Antiviral Agents/pharmacology/metabolism
PMC - PMC10747301
OTO - NOTNLM
OT  - berdazimer sodium
OT  - mechanism of action
OT  - molluscum contagiosum
OT  - molluscum contagiosum virus
OT  - nitric oxide
COIS- B.M.W. received research funding for this study from Novan; D.A.R., M.C. and 
      T.M.-C. are employees of Novan and receive a salary and stock or stock options.
EDAT- 2023/12/23 12:44
MHDA- 2023/12/25 06:43
PMCR- 2023/11/30
CRDT- 2023/12/23 01:29
PHST- 2023/10/04 00:00 [received]
PHST- 2023/11/23 00:00 [revised]
PHST- 2023/11/28 00:00 [accepted]
PHST- 2023/12/25 06:43 [medline]
PHST- 2023/12/23 12:44 [pubmed]
PHST- 2023/12/23 01:29 [entrez]
PHST- 2023/11/30 00:00 [pmc-release]
AID - v15122360 [pii]
AID - viruses-15-02360 [pii]
AID - 10.3390/v15122360 [doi]
PST - epublish
SO  - Viruses. 2023 Nov 30;15(12):2360. doi: 10.3390/v15122360.