PMID- 38140684
OWN - NLM
STAT- MEDLINE
DCOM- 20231225
LR  - 20231225
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 15
IP  - 12
DP  - 2023 Dec 16
TI  - Changes in the Murine Microbiome and Bacterial Extracellular Vesicle Production 
      in Response to Antibiotic Treatment and Norovirus Infection.
LID - 10.3390/v15122443 [doi]
LID - 2443
AB  - Norovirus infection is influenced by the presence of commensal bacteria, and both 
      human and murine norovirus (MNV) bind to these bacteria. These virus-bacterial 
      interactions, as well as MNV infection, promote the increased production of 
      bacterial extracellular vesicles (bEVs). However, no correlation has been made 
      between specific bacterial groups, their vesicles, and their impact on norovirus 
      infection. The current study evaluated the impact of select bacterial 
      compositions of murine microbiomes using antibiotic (ABX) cocktails on MNV 
      infection and bEV production. The goal of this research was to determine if 
      increases in bEVs following MNV infection in mice were associated with changes in 
      specific bacterial populations. Bacterial taxa were found to be differentially 
      abundant in both ABX-treated and untreated mice, with the greatest change in 
      bacterial taxa seen in mice treated with a broad-spectrum ABX cocktail. 
      Specifically, Lachnospiraeae were found to be differentially abundant between a 
      variety of treatment factors, including MNV infection. Overall, these results 
      demonstrate that MNV infection can alter the abundance of bacterial taxa within 
      the microbiota, as well as their production of extracellular vesicles, and that 
      the use of selective antibiotic treatments can allow the detection of viral 
      impacts on the microbiome that might otherwise be masked.
FAU - Mosby, Chanel A
AU  - Mosby CA
AUID- ORCID: 0000-0002-5848-5229
AD  - Microbiology and Cell Science Department, IFAS, University of Florida, 
      Gainesville, FL 32611, USA.
FAU - Long, Kendall J
AU  - Long KJ
AUID- ORCID: 0000-0003-3159-246X
AD  - Microbiology and Cell Science Department, IFAS, University of Florida, 
      Gainesville, FL 32611, USA.
FAU - Phillips, Matthew B
AU  - Phillips MB
AD  - Molecular Genetics and Microbiology Department, College of Medicine, University 
      of Florida, Gainesville, FL 32611, USA.
FAU - Bartel, Julia
AU  - Bartel J
AD  - Microbiology and Cell Science Department, IFAS, University of Florida, 
      Gainesville, FL 32611, USA.
FAU - Jones, Melissa K
AU  - Jones MK
AD  - Microbiology and Cell Science Department, IFAS, University of Florida, 
      Gainesville, FL 32611, USA.
LA  - eng
GR  - Hatch project 7004422/National Institute of Food and Agriculture/
GR  - Microbiology and Cell Science Department/University of Florida/
PT  - Journal Article
DEP - 20231216
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Mice
MH  - *Microbiota
MH  - *Caliciviridae Infections
MH  - Anti-Bacterial Agents/pharmacology/therapeutic use
PMC - PMC10747002
OTO - NOTNLM
OT  - 16S sequencing
OT  - Lachnospiraeae
OT  - MNV infection
OT  - OMV
OT  - bEV
OT  - bacterial extracellular vesicles
OT  - microbiome composition
OT  - murine norovirus
OT  - outer membrane vesicle
COIS- The authors declare no conflict of interest.
EDAT- 2023/12/23 12:42
MHDA- 2023/12/25 06:42
PMCR- 2023/12/16
CRDT- 2023/12/23 01:30
PHST- 2023/11/17 00:00 [received]
PHST- 2023/12/12 00:00 [revised]
PHST- 2023/12/15 00:00 [accepted]
PHST- 2023/12/25 06:42 [medline]
PHST- 2023/12/23 12:42 [pubmed]
PHST- 2023/12/23 01:30 [entrez]
PHST- 2023/12/16 00:00 [pmc-release]
AID - v15122443 [pii]
AID - viruses-15-02443 [pii]
AID - 10.3390/v15122443 [doi]
PST - epublish
SO  - Viruses. 2023 Dec 16;15(12):2443. doi: 10.3390/v15122443.