PMID- 3814150
OWN - NLM
STAT- MEDLINE
DCOM- 19870309
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 142
IP  - 2
DP  - 1987 Jan 30
TI  - A novel protein kinase activity in rabbit gastric gland cytosol.
PG  - 559-66
AB  - A novel protein kinase activity was characterized from the cytosolic fraction of 
      isolated rabbit gastric glands. The kinase phosphorylated a major 33,000 Da 
      endogenous protein (pp33) and was stimulated by Zn2+ and Mn2+ with Kact of 1.0 
      and 7.5 mM, respectively. Mg2+ and Ca2+ failed to stimulate any pp33 kinase 
      activity. The kinase utilized both ATP and GTP as phosphate donors with a Km of 
      10 microM for both. The pp33 protein displayed an isoelectric point of 7.5 to 7.8 
      and was phosphorylated predominantly on threonine residues. The kinase activity 
      is clearly differentiable from all reported kinase activities and appeared to be 
      enriched in rabbit gastric fundic mucosa. The results indicate that gastric 
      fundic mucosa contains a novel protein kinase activity.
FAU - Goldenring, J R
AU  - Goldenring JR
FAU - Oddsdottir, M
AU  - Oddsdottir M
FAU - Modlin, I M
AU  - Modlin IM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 42Z2K6ZL8P (Manganese)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Cytosol/*enzymology
MH  - Gastric Mucosa/*enzymology
MH  - In Vitro Techniques
MH  - Isoelectric Focusing
MH  - Manganese/pharmacology
MH  - Peptide Mapping
MH  - Phosphorylation
MH  - Protein Kinases/*analysis/metabolism
MH  - Protein-Tyrosine Kinases/analysis
MH  - Rabbits
MH  - Zinc/pharmacology
EDAT- 1987/01/30 00:00
MHDA- 1987/01/30 00:01
CRDT- 1987/01/30 00:00
PHST- 1987/01/30 00:00 [pubmed]
PHST- 1987/01/30 00:01 [medline]
PHST- 1987/01/30 00:00 [entrez]
AID - 0006-291X(87)90310-X [pii]
AID - 10.1016/0006-291x(87)90310-x [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1987 Jan 30;142(2):559-66. doi: 
      10.1016/0006-291x(87)90310-x.