PMID- 38141707
OWN - NLM
STAT- MEDLINE
DCOM- 20240208
LR  - 20240208
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 258
IP  - Pt 2
DP  - 2024 Feb
TI  - Conjugation of chitopentaose with beta-lactoglobulin using Maillard reaction, and 
      its effect on the allergic desensitization in vivo.
PG  - 128913
LID - S0141-8130(23)05812-9 [pii]
LID - 10.1016/j.ijbiomac.2023.128913 [doi]
AB  - The conjugation of chitopentaose (CHP) on beta-lactoglobulin (betaLg) via Maillard 
      reaction was used to desensitize betaLg. The stable betaLg-CHP conjugate (betaC-4) was 
      formed at 4 h incubation, which contains 5 CHP attached molecules and a 
      conjugated degree of 42 %. The conjugation promoted the thermal stability and 
      emulsifying properties of betaLg, and inhibited the immunoglobulin E (IgE) combining 
      capacity by decreasing the content of beta-sheet in betaLg. Moreover, betaLg-CHP 
      conjugates were imparted with anti-oxidant properties and anti-inflammatory 
      activities. Further, the combined action of inhibited IgE combining capacity and 
      anti-inflammatory activities improved the allergy desensitization in betaLg 
      sensitized mice. The results showed that overexpressed IgE and inflammatory 
      factors, unbalanced Th1-/Th2- immune cytokines were significantly attenuated 
      after betaLg was conjugated with CHP, avoiding the inflammatory lesions in spleen 
      and colon. Additionally, the adverse changes in gut microbiota were alleviated in 
      betaC-4 group with a decrease of Bacteroidetes and increase of Firmicutes at phylum 
      level and the probiotic bacteria of Lactobacillaceae was significantly improved 
      at the family level. Thus, the conjugation of CHP can desensitize allergic 
      reaction caused by betaLg.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Zhang, Xiaoning
AU  - Zhang X
AD  - School of Food Science & Engineering, Qilu University of Technology (Shandong 
      Academy of Science), Jinan 250353, China. Electronic address: 
      xiaoningzhang@126.com.
FAU - Chen, Meng
AU  - Chen M
AD  - Center for Disease Control and Prevention of Tengzhou City, Zaozhuang 277500, 
      China.
FAU - Wang, Ning
AU  - Wang N
AD  - School of Food Science & Engineering, Qilu University of Technology (Shandong 
      Academy of Science), Jinan 250353, China.
FAU - Luo, Juanjuan
AU  - Luo J
AD  - School of Food Science & Engineering, Qilu University of Technology (Shandong 
      Academy of Science), Jinan 250353, China.
FAU - Li, Meifeng
AU  - Li M
AD  - School of Public Health, Chengdu University of Traditional Chinese Medicine, 
      Chengdu 610075, China. Electronic address: meifeng.li@cdutcm.edu.cn.
FAU - Li, Sining
AU  - Li S
AD  - College of Food Science and Technology, Southwest Minzu University, Chengdu 
      610041, China. Electronic address: ruoly123@126.com.
FAU - Hemar, Yacine
AU  - Hemar Y
AD  - School of Natural Sciences, Massey University, Private Bag 11 222, Palmerston 
      North 4442, New Zealand.
LA  - eng
PT  - Journal Article
DEP - 20231221
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Lactoglobulins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Anti-Inflammatory Agents)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Lactoglobulins
MH  - Maillard Reaction
MH  - Immunoglobulin E
MH  - *Hypersensitivity
MH  - Anti-Inflammatory Agents
OTO - NOTNLM
OT  - Chitopentaose (CHP)
OT  - Desensitization
OT  - Gut microbiota
OT  - Maillard reaction
OT  - beta-Lactoglobulin (betaLg)
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/12/24 09:42
MHDA- 2024/02/08 06:43
CRDT- 2023/12/23 19:24
PHST- 2023/11/09 00:00 [received]
PHST- 2023/12/08 00:00 [revised]
PHST- 2023/12/18 00:00 [accepted]
PHST- 2024/02/08 06:43 [medline]
PHST- 2023/12/24 09:42 [pubmed]
PHST- 2023/12/23 19:24 [entrez]
AID - S0141-8130(23)05812-9 [pii]
AID - 10.1016/j.ijbiomac.2023.128913 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2024 Feb;258(Pt 2):128913. doi: 
      10.1016/j.ijbiomac.2023.128913. Epub 2023 Dec 21.