PMID- 38143256
OWN - NLM
STAT- MEDLINE
DCOM- 20240229
LR  - 20240229
IS  - 1861-0293 (Electronic)
IS  - 1340-3443 (Linking)
VI  - 78
IP  - 2
DP  - 2024 Mar
TI  - Kaempferol-3-O-(2''-O-galloyl-beta-D-glucopyranoside): a novel neuroprotective agent 
      from Diospryros kaki against cerebral ischemia-induced brain injury.
PG  - 312-327
LID - 10.1007/s11418-023-01765-z [doi]
AB  - Our previous study demonstrated neuroprotective and therapeutic effects of a 
      standardized flavonoid extract from leaves of Diospyros kaki L.f. (DK) on middle 
      cerebral artery occlusion-and-reperfusion (MCAO/R)-induced brain injury and its 
      underlying mechanisms. This study aimed to clarify flavonoid components 
      responsible for the effects of DK using in vitro and in vivo transient brain 
      ischemic models. Organotypic hippocampal slice cultures (OHSCs) subjected to 
      oxygen- and glucose-deprivation (OGD) were performed to evaluate in vitro 
      neuroprotective activity of DK extract and nine isolated flavonoid components. 
      MCAO/R mice were employed to elucidate in vivo neuroprotective effects of the 
      flavonoid component that exhibited the most potent neuroprotective effect in 
      OHSCs. DK extract and seven flavonoids [quercetin, isoquercetin, hyperoside, 
      quercetin-3-O-(2''-O-galloyl-beta-D-galactopyranoside), kaempferol, astragalin, and 
      kaempferol-3-O-(2''-O-galloyl-beta-D-glucopyranoside) compound (9)] attenuated 
      OGD-induced neuronal cell damage and compound (9) possessed the most potent 
      neuroprotective activity in OHSCs. The MCAO/R mice showed cerebral infarction, 
      massive weight loss, characteristic neurological symptoms, and deterioration of 
      neuronal cells in the brain. Compound (9) and a reference drugs, edaravone, 
      significantly attenuated these physical and neurological impairments. Compound 
      (9) mitigated the blood-brain barrier dysfunction and the change of glutathione 
      and malondialdehyde content in the MCAO mouse brain. Edaravone suppressed the 
      oxidative stress but did not significantly affect the blood-brain barrier 
      permeability. The present results indicated that compound (9) is a flavonoid 
      constituent of DK with a potent neuroprotective activity against transient 
      ischemia-induced brain damage and this action, at least in part, via preservation 
      of blood-brain barrier integrity and suppression of oxidative stress caused by 
      ischemic insult.
CI  - (c) 2023. The Author(s) under exclusive licence to The Japanese Society of 
      Pharmacognosy.
FAU - Nguyen, Loan Thanh Thi
AU  - Nguyen LTT
AD  - Department of Pharmacology and Biochemistry, National Institute of Medicinal 
      Materials, Hanoi, Vietnam.
AD  - Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam.
FAU - Le, Xoan Thi
AU  - Le XT
AUID- ORCID: 0000-0002-4471-3155
AD  - Department of Pharmacology and Biochemistry, National Institute of Medicinal 
      Materials, Hanoi, Vietnam. xoanle@nimm.org.vn.
FAU - Nguyen, Ha Thi
AU  - Nguyen HT
AD  - Department of Extraction Technology, Vietnam National Institute of Medicinal 
      Materials, Hanoi, Vietnam.
FAU - Nguyen, Tai Van
AU  - Nguyen TV
AD  - Department of Phytochemistry, National Institute of Medicinal Materials, Hanoi, 
      Vietnam.
FAU - Pham, Hang Nguyet Thi
AU  - Pham HNT
AD  - Department of Pharmacology and Biochemistry, National Institute of Medicinal 
      Materials, Hanoi, Vietnam.
FAU - Van Thi Pham, Anh
AU  - Van Thi Pham A
AD  - Department of Pharmacology, Hanoi Medical University, Hanoi, Vietnam.
FAU - Matsumoto, Kinzo
AU  - Matsumoto K
AD  - Graduate School of Pharmaceutical Sciences, Daiichi University of Pharmacy, 
      Fukuoka, Japan.
LA  - eng
GR  - 01/2022/HD-DTCS-DLSH/National Institute of Medicinal Materials/
PT  - Journal Article
DEP - 20231224
PL  - Japan
TA  - J Nat Med
JT  - Journal of natural medicines
JID - 101518405
RN  - 0 (Neuroprotective Agents)
RN  - 9IKM0I5T1E (Quercetin)
RN  - S798V6YJRP (Edaravone)
RN  - 0 (Kaempferols)
RN  - 0 (Flavonoids)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Neuroprotective Agents/pharmacology/therapeutic use
MH  - Quercetin/pharmacology/therapeutic use
MH  - Edaravone/therapeutic use
MH  - *Diospyros
MH  - Kaempferols/pharmacology/therapeutic use
MH  - *Brain Ischemia/drug therapy
MH  - Cerebral Infarction/drug therapy
MH  - Flavonoids/pharmacology
MH  - *Reperfusion Injury/drug therapy
MH  - Oxygen
MH  - *Brain Injuries/drug therapy
OTO - NOTNLM
OT  - Diospyros kaki
OT  - Blood-brain barrier integrity
OT  - Cerebral ischemia
OT  - Kaempferol-3-O-(2''-O-galloyl-beta-d-glucopyranoside)
OT  - Neuroprotective effect
OT  - Oxidative stress
EDAT- 2023/12/25 00:42
MHDA- 2024/02/29 06:43
CRDT- 2023/12/24 23:13
PHST- 2023/08/22 00:00 [received]
PHST- 2023/11/16 00:00 [accepted]
PHST- 2024/02/29 06:43 [medline]
PHST- 2023/12/25 00:42 [pubmed]
PHST- 2023/12/24 23:13 [entrez]
AID - 10.1007/s11418-023-01765-z [pii]
AID - 10.1007/s11418-023-01765-z [doi]
PST - ppublish
SO  - J Nat Med. 2024 Mar;78(2):312-327. doi: 10.1007/s11418-023-01765-z. Epub 2023 Dec 
      24.