PMID- 38146030
OWN - NLM
STAT- MEDLINE
DCOM- 20240227
LR  - 20240306
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 41
IP  - 2
DP  - 2024 Feb
TI  - Risks of using mitoquinone during in vitro maturation and its potential 
      protective effects against lipotoxicity-induced oocyte mitochondrial stress.
PG  - 371-383
LID - 10.1007/s10815-023-02994-7 [doi]
AB  - PURPOSE: Oxidative stress and mitochondrial dysfunction play central roles in 
      reduced oocyte quality and infertility in obese patients. Mitochondria-targeted 
      treatments containing co-enzyme Q10 such as mitoquinone (MitoQ) can increase 
      mitochondrial antioxidative capacity; however, their safety and efficiency when 
      supplemented to oocytes under lipotoxic conditions have not been described. 
      METHODS: We tested the effect of different concentrations of MitoQ or its 
      cationic carrier (TPP) (0, 0.1, 0.5, 1.0 muM each) during bovine oocyte IVM. Then, 
      we tested the protective capacity of MitoQ (0.1 muM) against palmitic acid 
      (PA)-induced lipotoxicity and mitochondrial dysfunction in oocytes. RESULTS: 
      Exposure to MitoQ, or TPP only, at 1 muM significantly (P<0.05) reduced oocyte 
      mitochondrial inner membrane potential (JC-1 staining) and resulted in reduced 
      cleavage and blastocyst rates compared with solvent control. Lower concentrations 
      of MitoQ or TPP had no effects on embryo development under control (PA-free) 
      conditions. As expected, PA increased the levels of MMP and ROS in oocytes 
      (CellROX staining) and reduced cleavage and blastocyst rates compared with the 
      controls (P<0.05). These negative effects were ameliorated by 0.1 muM MitoQ. In 
      contrast, 0.1 muM TPP alone had no protective effects. MitoQ also normalized the 
      expression of HSP10 and TFAM, and partially normalized HSP60 in the produced 
      blastocysts, indicating at least a partial alleviation of PA-induced 
      mitochondrial stress. CONCLUSION: Oocyte exposure to MitoQ may disturb 
      mitochondrial bioenergetic functions and developmental capacity due to a 
      TPP-induced cationic overload. A fine-tuned concentration of MitoQ can protect 
      against lipotoxicity-induced mitochondrial stress during IVM and restore 
      developmental competence and embryo quality.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Marei, Waleed F A
AU  - Marei WFA
AUID- ORCID: 0000-0002-8498-8903
AD  - Gamete Research Centre, Department of Veterinary Sciences, University of Antwerp, 
      Antwerp, Belgium. waleed.marei@uantwerpen.be.
AD  - Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, 
      Giza, Egypt. waleed.marei@uantwerpen.be.
FAU - Mohey-Elsaeed, Omnia
AU  - Mohey-Elsaeed O
AD  - Department of Histology and Cytology, Faculty of Veterinary Medicine, Cairo 
      University, Giza, Egypt.
AD  - Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, 
      Belgium.
FAU - Pintelon, Isabel
AU  - Pintelon I
AD  - Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, 
      Belgium.
FAU - Leroy, Jo L M R
AU  - Leroy JLMR
AD  - Gamete Research Centre, Department of Veterinary Sciences, University of Antwerp, 
      Antwerp, Belgium.
LA  - eng
GR  - R01 OH004216/OH/NIOSH CDC HHS/United States
PT  - Journal Article
DEP - 20231226
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 47BYS17IY0 (mitoquinone)
RN  - 0 (Organophosphorus Compounds)
RN  - 1339-63-5 (Ubiquinone)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Cattle
MH  - *In Vitro Oocyte Maturation Techniques/methods
MH  - Oocytes
MH  - Blastocyst/metabolism
MH  - Embryonic Development
MH  - Mitochondria/metabolism
MH  - *Mitochondrial Diseases
MH  - *Organophosphorus Compounds
MH  - Ubiquinone/*analogs & derivatives
PMC - PMC10894804
OTO - NOTNLM
OT  - CoQ10
OT  - Embryo
OT  - IVF
OT  - MitoQ
OT  - Obesity
OT  - Oocyte
OT  - Oxidative stress
COIS- The authors declare no competing interests.
EDAT- 2023/12/26 00:42
MHDA- 2024/02/27 06:45
PMCR- 2025/02/01
CRDT- 2023/12/25 23:22
PHST- 2023/07/13 00:00 [received]
PHST- 2023/11/17 00:00 [accepted]
PHST- 2025/02/01 00:00 [pmc-release]
PHST- 2024/02/27 06:45 [medline]
PHST- 2023/12/26 00:42 [pubmed]
PHST- 2023/12/25 23:22 [entrez]
AID - 10.1007/s10815-023-02994-7 [pii]
AID - 2994 [pii]
AID - 10.1007/s10815-023-02994-7 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2024 Feb;41(2):371-383. doi: 10.1007/s10815-023-02994-7. 
      Epub 2023 Dec 26.