PMID- 38147983
OWN - NLM
STAT- MEDLINE
DCOM- 20231228
LR  - 20231228
IS  - 1872-2075 (Electronic)
IS  - 1000-3061 (Linking)
VI  - 39
IP  - 12
DP  - 2023 Dec 25
TI  - [Eukaryotic expression of GP5 and M protein of porcine reproductive and 
      respiratory syndrome virus and immunogenicity evaluation].
PG  - 4809-4823
LID - 10.13345/j.cjb.230188 [doi]
AB  - In order to understand the prevalence and evolution of porcine reproductive and 
      respiratory syndrome virus (PRRSV) in China and to develop subunit vaccine 
      against the epidemic lineage, the genetic evolution analysis of PRRSV strains 
      isolated in China from 2001 to 2021 was performed. The representative strains of 
      the dominant epidemic lineage were selected to optimize the membrane protein GP5 
      and M nucleotide sequences, which were used, with the interferon and the Fc 
      region of immunoglobulin, to construct the eukaryotic expression plasmids 
      pCDNA3.4-IFNalpha-GP5-Fc and pCDNA3.4-IFNalpha-M-Fc. Subsequently, the recombinant 
      proteins IFNalpha-GP5-Fc and IFNalpha-M-Fc were expressed by HEK293T eukaryotic 
      expression system. The two recombinant proteins were mixed with ISA206VG adjuvant 
      to immunize weaned piglets. The humoral immunity level was evaluated by ELISA and 
      neutralization test, and the cellular immunity level was detected by ELISPOT 
      test. The results showed that the NADC30-like lineage was the main epidemic 
      lineage in China in recent years, and the combination of IFNalpha-GP5-Fc and 
      IFNalpha-M-Fc could induce high levels of antibody and cellular immunity in piglets. 
      This study may facilitate the preparation of a safer and more effective new PRRSV 
      subunit vaccine.
FAU - Lou, Huicong
AU  - Lou H
AD  - Animal Science and Technology College, Guangxi University, Nanning 530004, 
      Guangxi, China.
AD  - Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Lin, Runshan
AU  - Lin R
AD  - Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
AD  - University of Chinese Academy of Sciences, Beijing 100049, China.
FAU - Li, Yabo
AU  - Li Y
AD  - Animal Science and Technology College, Guangxi University, Nanning 530004, 
      Guangxi, China.
AD  - Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Zhao, Yuna
AU  - Zhao Y
AD  - Animal Science and Technology College, Guangxi University, Nanning 530004, 
      Guangxi, China.
AD  - Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Jiao, Pengtao
AU  - Jiao P
AD  - Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Luo, Tingrong
AU  - Luo T
AD  - Animal Science and Technology College, Guangxi University, Nanning 530004, 
      Guangxi, China.
FAU - Liu, Wenjun
AU  - Liu W
AD  - Animal Science and Technology College, Guangxi University, Nanning 530004, 
      Guangxi, China.
AD  - Key Laboratory of Pathogenic Microbiology and Immunology, Institute of 
      Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
AD  - University of Chinese Academy of Sciences, Beijing 100049, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Vaccines)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Subunit)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Swine
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - *Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - HEK293 Cells
MH  - Viral Envelope Proteins/genetics
MH  - Antibodies, Viral
MH  - *Viral Vaccines/genetics
MH  - Recombinant Proteins
MH  - Vaccines, Subunit
OTO - NOTNLM
OT  - GP5 recombinant protein
OT  - M recombinant protein
OT  - genetic evolution analysis
OT  - immunogenicity evaluation
OT  - porcine reproductive and respiratory syndrome virus (PRRS)
EDAT- 2023/12/27 00:41
MHDA- 2023/12/28 06:42
CRDT- 2023/12/26 19:42
PHST- 2023/12/28 06:42 [medline]
PHST- 2023/12/27 00:41 [pubmed]
PHST- 2023/12/26 19:42 [entrez]
AID - 10.13345/j.cjb.230188 [doi]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2023 Dec 25;39(12):4809-4823. doi: 
      10.13345/j.cjb.230188.