PMID- 38148711
OWN - NLM
STAT- MEDLINE
DCOM- 20240311
LR  - 20240311
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 39
IP  - 4
DP  - 2024 Apr
TI  - Repeated intratracheal instillation of whole-cigarette smoke condensate to assess 
      lung damage in a rat model.
PG  - 2304-2315
LID - 10.1002/tox.24113 [doi]
AB  - Cigarette smoke induces an inflammatory response in the lungs by recruiting 
      inflammatory cells, leading to lung diseases such as lung cancer, chronic 
      obstructive pulmonary disease, and pulmonary fibrosis. Existing inhalation 
      exposure methods for assessing the adverse effects of cigarette smoke require 
      expensive equipment and are labor-intensive. Therefore, we attempted to develop a 
      novel method to assess these adverse effects using intratracheal instillation 
      (ITI) of whole cigarette smoke condensate (WCSC). The WCSC (0, 5, 10, or 
      20 mg/mL) was administered by ITI once daily for 6 or 12 days using an automatic 
      video instillator. Repeated WCSC ITI increased the lung weight, and monocyte 
      chemoattractant protein-1 (MCP-1), neutrophil, and lymphocyte levels within 
      bronchoalveolar lavage fluid compared to the control. In the histopathological 
      analysis of the lung tissue, a mild inflammatory response was observed in the 6 
      and 12 days 20 mg/mL WCSC exposure groups. The genome-wide RNA-seq expression 
      patterns revealed that inflammatory and immune response-related genes, such as 
      the chemokine signaling pathway, Th1/Th2 cell differentiation, and 
      cytokine-cytokine receptor interaction, were employed following WCSC exposure. In 
      addition, MCP-1 was time-dependent and increased in the 10 mg/mL exposure group 
      compared to the control group. These results suggested that the WCSC might induce 
      the potential pulmonary inflammatory response. Furthermore, we proposed that ITI 
      may be a rapid and effective method of evaluating the adverse effects of WCSC 
      within a short exposure period (less than 2 weeks), and it can be used to 
      evaluate cigarette inhalation toxicity studies as an alternative method.
CI  - (c) 2023 The Authors. Environmental Toxicology published by Wiley Periodicals LLC.
FAU - Kim, Jinhee
AU  - Kim J
AD  - Inhalation Toxicology Research Group, Korea Institute of Toxicology (KIT), 
      Jeongeup, Jeonbuk, Republic of Korea.
FAU - Cho, Yoon
AU  - Cho Y
AD  - Inhalation Toxicology Research Group, Korea Institute of Toxicology (KIT), 
      Jeongeup, Jeonbuk, Republic of Korea.
FAU - Oh, Gi-Jun
AU  - Oh GJ
AD  - Inhalation Toxicology Research Group, Korea Institute of Toxicology (KIT), 
      Jeongeup, Jeonbuk, Republic of Korea.
FAU - Park, Hae-Bin
AU  - Park HB
AD  - Inhalation Toxicology Research Group, Korea Institute of Toxicology (KIT), 
      Jeongeup, Jeonbuk, Republic of Korea.
FAU - Yang, Mi Jin
AU  - Yang MJ
AD  - Inhalation Toxicology Research Group, Korea Institute of Toxicology (KIT), 
      Jeongeup, Jeonbuk, Republic of Korea.
FAU - Park, Chul-Min
AU  - Park CM
AD  - Inhalation Toxicology Research Group, Korea Institute of Toxicology (KIT), 
      Jeongeup, Jeonbuk, Republic of Korea.
FAU - Kim, Yong-Hyun
AU  - Kim YH
AD  - Inhalation Toxicology Research Group, Korea Institute of Toxicology (KIT), 
      Jeongeup, Jeonbuk, Republic of Korea.
AD  - Department of Environment & Energy, Jeonbuk National University, Jeonju, Jeonbuk, 
      Republic of Korea.
FAU - Choi, Kyung-Chul
AU  - Choi KC
AUID- ORCID: 0000-0002-1925-1198
AD  - Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, 
      Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
FAU - Go, Ryeo-Eun
AU  - Go RE
AD  - Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, 
      Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
FAU - Kim, Min-Seok
AU  - Kim MS
AUID- ORCID: 0000-0001-8431-1516
AD  - Inhalation Toxicology Research Group, Korea Institute of Toxicology (KIT), 
      Jeongeup, Jeonbuk, Republic of Korea.
LA  - eng
GR  - 21203MFDS318/Ministry of Food and Drug Safety, Republic of Korea/
PT  - Journal Article
DEP - 20231226
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Cigarette Smoking
MH  - Lung
MH  - *Pulmonary Disease, Chronic Obstructive/metabolism
MH  - *Lung Diseases/pathology
MH  - Bronchoalveolar Lavage Fluid
OTO - NOTNLM
OT  - alternative method
OT  - cigarette smoke
OT  - intratracheal instillation
OT  - lung toxicity
OT  - whole-cigarette smoke condensate
EDAT- 2023/12/27 06:41
MHDA- 2024/03/11 06:42
CRDT- 2023/12/27 02:53
PHST- 2023/08/29 00:00 [revised]
PHST- 2023/03/08 00:00 [received]
PHST- 2023/12/11 00:00 [accepted]
PHST- 2024/03/11 06:42 [medline]
PHST- 2023/12/27 06:41 [pubmed]
PHST- 2023/12/27 02:53 [entrez]
AID - 10.1002/tox.24113 [doi]
PST - ppublish
SO  - Environ Toxicol. 2024 Apr;39(4):2304-2315. doi: 10.1002/tox.24113. Epub 2023 Dec 
      26.