PMID- 38153588
OWN - NLM
STAT- MEDLINE
DCOM- 20231229
LR  - 20240116
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Linking)
VI  - 191
IP  - 1
DP  - 2023 Dec 28
TI  - A liquid crystal-based biosensor for sensitive detection of tumor necrosis 
      factor-alpha.
PG  - 55
LID - 10.1007/s00604-023-06125-y [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a cytokine secreted by the macrophages and 
      Th1 cells of the immune system in response to inflammation. Given its 
      significance as a biomarker with elevated levels in physiological fluids in 
      various conditions, there is an increasing demand for a simple and accurate TNF-alpha 
      detection strategy. In this article, we present a liquid crystal (LC)-based 
      biosensor developed for sensitive TNF-alpha detection. The biosensor operates as 
      follows: TNF-alpha and detection antibodies (DAbs) form complexes during 
      preincubation. These complexes then bind with the surface-immobilized capture 
      antibodies (CAbs), facilitating the antigen-antibody reaction between the CAbs 
      and the TNF-alpha/DAb complexes. This target recognition interaction alters the 
      surface topography, disrupting the vertical orientation of LCs produced by 
      dimethyloctadecyl[3-(trimethoxysilyl)-propyl]ammonium chloride. The orientational 
      change in the LCs can be easily visualized with a polarized optical microscope, 
      resulting in brighter images as TNF-alpha levels rise. Our results demonstrated a 
      linear range of 5.00-500 pg/mL, with a limit of detection and limit of 
      quantification being 1.08 and 3.56 pg/mL, respectively. Recovery experiments on 
      diluted saliva samples produced reasonable results, with TNF-alpha recoveries ranging 
      from 97.1% +/- 2.58% to 107% +/- 5.95%.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Ryu, Je-Jin
AU  - Ryu JJ
AD  - Department of Chemistry, Gachon University, Seongnam-daero 1342, Sujeong-Gu, 
      Seongnam-Si, Gyeonggi-Do, 13120, Republic of Korea.
FAU - Jang, Chang-Hyun
AU  - Jang CH
AUID- ORCID: 0000-0003-3165-4747
AD  - Department of Chemistry, Gachon University, Seongnam-daero 1342, Sujeong-Gu, 
      Seongnam-Si, Gyeonggi-Do, 13120, Republic of Korea. chjang4u@gachon.ac.kr.
LA  - eng
GR  - GCU-202110460001/Gachon University/
GR  - NRF-2019R1A2C1003862/National Institute for International Education/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231228
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
RN  - 0 (Antibodies)
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Antibodies
MH  - Antibodies, Immobilized
MH  - Cytokines
MH  - Liquid Crystals
MH  - *Tumor Necrosis Factor-alpha/analysis
MH  - Humans
MH  - *Biosensing Techniques
OTO - NOTNLM
OT  - Antibody
OT  - Biosensor
OT  - Liquid crystal
OT  - Polarized optical microscope
OT  - Tumor necrosis factor-alpha
EDAT- 2023/12/28 12:42
MHDA- 2023/12/29 06:43
CRDT- 2023/12/28 11:21
PHST- 2023/08/18 00:00 [received]
PHST- 2023/11/26 00:00 [accepted]
PHST- 2023/12/29 06:43 [medline]
PHST- 2023/12/28 12:42 [pubmed]
PHST- 2023/12/28 11:21 [entrez]
AID - 10.1007/s00604-023-06125-y [pii]
AID - 10.1007/s00604-023-06125-y [doi]
PST - epublish
SO  - Mikrochim Acta. 2023 Dec 28;191(1):55. doi: 10.1007/s00604-023-06125-y.