PMID- 38155907 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20231230 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 14 DP - 2023 TI - Radioprotective potential of whey protein against gamma irradiation-induced lingual damage. PG - 1293230 LID - 10.3389/fphar.2023.1293230 [doi] LID - 1293230 AB - Introduction: Ionizing radiation (IR) is effectively used in the treatment of oral malignancies; however, it might also significantly harm the surrounding tissues. Whey protein isolate (WP) is a protein derived from milk that exhibits a wide range of bioactivities. Therefore, the present research aimed to delineate the mitigating impact of WP against gamma irradiation-induced lingual damage. Methods: Rats were randomized into 5 groups: Control (saline, orally, 14 days), WP (WP; 0.5 g/kg b. w., orally, 14 days), IR (saline, orally, 14 days, exposed to 6 and 3 Gy on days 4 and 6, respectively), WP+IR (WP was given orally for 14 days before and after IR exposure; exposed to 6 and 3 Gy on days 4 and 6, respectively), and IR+WP (WP, orally, started 24 h after 1(st) IR exposure till the end of the experiment) groups. Samples were collected at two-time intervals (on the 7(th) and 14(th) days). Results and Discussion: Oxidative stress was stimulated upon IR exposure in tongue, indicated by boosted malondialdehyde (MDA) level, along with a decrease in the total antioxidant capacity (TAC) level, superoxide dismutase (SOD), and catalase (CAT) activities. Additionally, IR exposure depicted an increase of serum IgE, inflammatory cytokines, including tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-6, along with overexpression mRNA levels of nuclear factor kappa-B transcription factor/p65 (NF-kappaB/p65), and down-regulation of nuclear factor erythroid 2-related factor 2 (NRF2) and heme oxygenase (HO-1) mRNA levels in tongue tissue. Moreover, IR triggered alterations in lingual histological architecture. The antioxidant and anti-inflammatory properties of WP mitigated oxidative damage, inflammation, and desquamation that were brought on following IR exposure. The protective administration of WP markedly decreases IR-induced lingual harm compared to the mitigation protocol. Our findings recommend WP supplements to the diets of cancer patients undergoing IR that might aid radioprotective effects. CI - Copyright (c) 2023 Hassan, Abdeen, Abdelrahman, Abdo, Mohammed, Abdeen, Abdelkader, Olga, Fericean, Ibrahim, Ghamry, Elgendy, Sorour, Eldeeb, Ahmed, Rashed and Bikheet. FAU - Hassan, Hanaa M AU - Hassan HM AD - Department of Agricultural Chemistry, Faculty of Agriculture, Minia University, Minia, Egypt. FAU - Abdeen, Asmaa M AU - Abdeen AM AD - Department of Oral Biology, Faculty of Dentistry, Minia University, Minia, Egypt. FAU - Abdelrahman, Ibrahim Y AU - Abdelrahman IY AD - Department of Radiation Biology, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt. FAU - Abdo, Walied AU - Abdo W AD - Department of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt. FAU - Mohammed, Saher S AU - Mohammed SS AD - Department of Oral Biology, Faculty of Dentistry, Minia University, Minia, Egypt. FAU - Abdeen, Ahmed AU - Abdeen A AD - Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt. FAU - Abdelkader, Afaf AU - Abdelkader A AD - Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University, Benha, Egypt. FAU - Olga, Rada AU - Olga R AD - Department of Biology and Plant Protection, Faculty of Agriculture, University of Life Sciences, Timisoara, Romania. FAU - Fericean, Liana AU - Fericean L AD - Department of Biology and Plant Protection, Faculty of Agriculture, University of Life Sciences, Timisoara, Romania. FAU - Ibrahim, Samah F AU - Ibrahim SF AD - Department of Clinical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Ghamry, Heba I AU - Ghamry HI AD - Nutrition and Food Sciences, Department of Home Economics, Faculty of Home Economics, King Khalid University, Abha, Saudi Arabia. FAU - Elgendy, Farouk S AU - Elgendy FS AD - Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University, Benha, Egypt. FAU - Sorour, Safwa M AU - Sorour SM AD - Department of Pharmacology, Faculty of Medicine, Benha University, Benha, Egypt. FAU - Eldeeb, Abeer A AU - Eldeeb AA AD - Department of Pharmacology, Faculty of Medicine, Benha University, Benha, Egypt. FAU - Ahmed, Osama AU - Ahmed O AD - Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt. FAU - Rashed, Fatema AU - Rashed F AD - Department of Basic Medical and Dental Science, Faculty of Dentistry, Zarqaa University, Zarqaa, Jordan. FAU - Bikheet, Maha M AU - Bikheet MM AD - Dairy Science Department, Faculty of Agriculture, Minia University, Minia, Egypt. LA - eng PT - Journal Article DEP - 20231213 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC10753789 OTO - NOTNLM OT - inflammation OT - ionizing radiation OT - mRNA expression OT - oxidative damage OT - tongue OT - whey protein COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2024/01/02 10:02 MHDA- 2024/01/02 10:03 PMCR- 2023/12/13 CRDT- 2023/12/29 03:41 PHST- 2023/09/12 00:00 [received] PHST- 2023/11/17 00:00 [accepted] PHST- 2024/01/02 10:03 [medline] PHST- 2024/01/02 10:02 [pubmed] PHST- 2023/12/29 03:41 [entrez] PHST- 2023/12/13 00:00 [pmc-release] AID - 1293230 [pii] AID - 10.3389/fphar.2023.1293230 [doi] PST - epublish SO - Front Pharmacol. 2023 Dec 13;14:1293230. doi: 10.3389/fphar.2023.1293230. eCollection 2023.