PMID- 38158877
OWN - NLM
STAT- Publisher
LR  - 20231230
IS  - 1942-7611 (Electronic)
IS  - 1942-7603 (Linking)
DP  - 2023 Dec 30
TI  - Quantitative analysis of tetrahydrocannabinol isomers and other toxicologically 
      relevant drugs in blood.
LID - 10.1002/dta.3632 [doi]
AB  - A multi-analyte liquid chromatography-tandem mass spectrometry (LC-MS/MS) method 
      is described, involving the separation of delta-9-tetrahydrocannabinol 
      (delta-9-THC) and delta-8-THC in addition to other commonly encountered drugs and 
      metabolites. Briefly, sample preparation involved an alkaline liquid-liquid 
      extraction (methyl tert-butyl ether) of blood (100 mul). The solvent layer was 
      transferred, evaporated to dryness, reconstituted, and samples then separated on 
      an Agilent Poroshell 120 EC-C18 100 A (50 mm x 3.0 mm, 2.7 mum) analytical column 
      using a multi-step gradient elution of 50 mM ammonium formate in water (pH 3.5) 
      and 0.1% formic acid in methanol over 14 min. A SCIEX Triple Quad 6500+ system 
      operating in scheduled multiple reaction monitoring and positive electrospray 
      ionization was used for detection. There were no interferences, and matrix 
      effects were generally acceptable (+/-20% of neat response). Linearity was achieved 
      within the calibration range, including methylamphetamine (MA) (10-1000 ng/ml), 
      3,4-methylenedioxy-N-methylamphetamine (MDMA) (10-1,000 ng/ml), cocaine 
      (10-1000 ng/ml), and two THC isomers (1-100 ng/ml). Accuracies of MA, MDMA, 
      cocaine, and two THC isomers were 3.6 to 8.9%, -1.2 to 4%, -5.3 to 5.8%, and -11 
      to 14%, respectively; while precision estimates of the same were 1.6 to 5.4%, 1.7 
      to 5.3%, 1.2 to 4.5%, and 2 to 10%, respectively. Autosampler stability and 
      dilution integrity were within acceptable limits, and no carryover was detected 
      at the limit of detection. This validated LC-MS/MS method made the routine 
      identification of both delta-9-THC and delta-8-THC in blood possible.
CI  - (c) 2023 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.
FAU - Mantinieks, Dylan
AU  - Mantinieks D
AUID- ORCID: 0000-0002-8943-8994
AD  - Department of Forensic Medicine, School of Public Health and Preventive Medicine, 
      Monash University, Southbank, Victoria, Australia.
AD  - Victorian Institute of Forensic Medicine, Southbank, Victoria, Australia.
FAU - Di Rago, Matthew
AU  - Di Rago M
AUID- ORCID: 0000-0003-3490-5214
AD  - Department of Forensic Medicine, School of Public Health and Preventive Medicine, 
      Monash University, Southbank, Victoria, Australia.
AD  - Victorian Institute of Forensic Medicine, Southbank, Victoria, Australia.
FAU - Drummer, Olaf H
AU  - Drummer OH
AUID- ORCID: 0000-0001-8953-0312
AD  - Department of Forensic Medicine, School of Public Health and Preventive Medicine, 
      Monash University, Southbank, Victoria, Australia.
AD  - Victorian Institute of Forensic Medicine, Southbank, Victoria, Australia.
FAU - Glowacki, Linda
AU  - Glowacki L
AUID- ORCID: 0000-0002-7161-7513
AD  - Victorian Institute of Forensic Medicine, Southbank, Victoria, Australia.
FAU - Schumann, Jennifer
AU  - Schumann J
AUID- ORCID: 0000-0002-8870-6272
AD  - Department of Forensic Medicine, School of Public Health and Preventive Medicine, 
      Monash University, Southbank, Victoria, Australia.
AD  - Victorian Institute of Forensic Medicine, Southbank, Victoria, Australia.
FAU - Gerostamoulos, Dimitri
AU  - Gerostamoulos D
AUID- ORCID: 0000-0002-7958-8235
AD  - Department of Forensic Medicine, School of Public Health and Preventive Medicine, 
      Monash University, Southbank, Victoria, Australia.
AD  - Victorian Institute of Forensic Medicine, Southbank, Victoria, Australia.
LA  - eng
GR  - Department of Education, Australian Governement/
GR  - Monash University/
PT  - Journal Article
DEP - 20231230
PL  - England
TA  - Drug Test Anal
JT  - Drug testing and analysis
JID - 101483449
SB  - IM
OTO - NOTNLM
OT  - amphetamines
OT  - cannabinoids
OT  - cocaine
OT  - driving under the influence
OT  - forensic toxicology
EDAT- 2024/01/02 11:43
MHDA- 2024/01/02 11:43
CRDT- 2023/12/30 07:35
PHST- 2023/12/09 00:00 [revised]
PHST- 2023/09/19 00:00 [received]
PHST- 2023/12/11 00:00 [accepted]
PHST- 2024/01/02 11:43 [medline]
PHST- 2024/01/02 11:43 [pubmed]
PHST- 2023/12/30 07:35 [entrez]
AID - 10.1002/dta.3632 [doi]
PST - aheadofprint
SO  - Drug Test Anal. 2023 Dec 30. doi: 10.1002/dta.3632.