PMID- 38159155
OWN - NLM
STAT- MEDLINE
DCOM- 20240103
LR  - 20240116
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Linking)
VI  - 191
IP  - 1
DP  - 2023 Dec 30
TI  - Development of competitive and noncompetitive lateral flow immunoassays for 
      pendimethalin using synthetic peptides.
PG  - 68
LID - 10.1007/s00604-023-06151-w [doi]
AB  - Peptidomimetic and anti-immunocomplex peptides can be easily isolated from phage 
      display libraries, and can be used as alternatives to chemical competing haptens 
      to improve the sensitivity of small molecule immunoassay. In this work, 16 
      peptidomimetics and 7 anti-immunocomplex peptides of pendimethalin (PND) were 
      obtained from cyclic 7-, 8-, 9-, and 10-residue peptide phage libraries. 
      Peptidomimetic EJ-2 (CMFTGTDFPC) with the highest sensitivity in competitive 
      phage enzyme-linked immunosorbent assay (ELISA) and immunocomplex peptide EF-30 
      (CNPGWPPIPC) with the highest sensitivity in noncompetitive phage ELISA were 
      selected to prepare phage-free peptides with GGGSSK-biotin at the C-terminus. 
      Competitive and noncompetitive lateral flow immunoassays (CLFIA and NLFIA) were 
      developed by using the phage-free peptides. After optimization, the CLFIA and 
      NLFIA showed visual limit of detections (vLODs) of 5 ng/mL and 2.5 ng/mL, 
      respectively, which were improved two- and fourfold compared with a LFIA based on 
      chemical hapten. The NLFIA showed better sensitivity than CLFIA in the detection 
      of spiked samples, and can meet the detection requirements for agro-products 
      regulated by EU and China. The detection results of CLFIA and NLFIA for blind 
      samples were consistent with that of ultra performance liquid 
      chromatography/tandem mass spectrometry.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Huang, Lianrun
AU  - Huang L
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, 
      China.
AD  - State & Local Joint Engineering Research Center of Green Pesticide Invention and 
      Application, Nanjing, 210095, China.
FAU - Wang, Guixiu
AU  - Wang G
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, 
      China.
AD  - State & Local Joint Engineering Research Center of Green Pesticide Invention and 
      Application, Nanjing, 210095, China.
FAU - Wu, Yanling
AU  - Wu Y
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, 
      China.
AD  - State & Local Joint Engineering Research Center of Green Pesticide Invention and 
      Application, Nanjing, 210095, China.
FAU - Wang, Zhijia
AU  - Wang Z
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, 
      China.
AD  - State & Local Joint Engineering Research Center of Green Pesticide Invention and 
      Application, Nanjing, 210095, China.
FAU - Ding, Yuan
AU  - Ding Y
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, 
      China.
AD  - State & Local Joint Engineering Research Center of Green Pesticide Invention and 
      Application, Nanjing, 210095, China.
FAU - Liang, Hongwu
AU  - Liang H
AD  - Inner Mongolia Key Laboratory of Environmental Pollution Control & Waste Resource 
      Reuse, School of Ecology and Environment, Inner Mongolia University, Hohhot, 
      010021, China.
FAU - Hua, Xiude
AU  - Hua X
AUID- ORCID: 0000-0003-0557-1892
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, 
      China. huaxiude@njau.edu.cn.
AD  - State & Local Joint Engineering Research Center of Green Pesticide Invention and 
      Application, Nanjing, 210095, China. huaxiude@njau.edu.cn.
LA  - eng
GR  - CX(22)3075/Jiangsu Agriculture Science and Technology Innovation Fund/
GR  - 2022YFSH0050/Inner Mongolia University of Science and Technology Plan/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231230
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
RN  - VL6L14C06U (pendimethalin)
RN  - 0 (Peptidomimetics)
RN  - 0 (Peptides)
RN  - 0 (Peptide Library)
SB  - IM
MH  - *Peptidomimetics
MH  - Peptides/chemistry
MH  - Immunoassay/methods
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Peptide Library
MH  - *Bacteriophages
OTO - NOTNLM
OT  - Later flow immunoassay
OT  - Pendimethalin
OT  - Phage-display peptides
OT  - Small molecule pollutant
EDAT- 2024/01/02 11:43
MHDA- 2024/01/03 09:43
CRDT- 2023/12/30 11:05
PHST- 2023/10/05 00:00 [received]
PHST- 2023/12/11 00:00 [accepted]
PHST- 2024/01/03 09:43 [medline]
PHST- 2024/01/02 11:43 [pubmed]
PHST- 2023/12/30 11:05 [entrez]
AID - 10.1007/s00604-023-06151-w [pii]
AID - 10.1007/s00604-023-06151-w [doi]
PST - epublish
SO  - Mikrochim Acta. 2023 Dec 30;191(1):68. doi: 10.1007/s00604-023-06151-w.