PMID- 38160652
OWN - NLM
STAT- MEDLINE
DCOM- 20240112
LR  - 20240112
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 165
DP  - 2024 Jan
TI  - Lipoproteins are key immunostimulatory components of Bacillus species for 
      dendritic cell maturation and activation.
PG  - 82-91
LID - S0161-5890(23)00248-1 [pii]
LID - 10.1016/j.molimm.2023.12.009 [doi]
AB  - Dendritic cells (DCs) play an important role in immunity by sensing and 
      responding to invasive microbes. Bacillus species are rod-shaped sporulating 
      bacteria that include the pathogenic Bacillus cereus and commensal Bacillus 
      subtilis. Although the interaction between DC and these two Bacillus species has 
      been studied, their key structural component that prompts DC activation is poorly 
      understood. Here, we investigated the two Bacillus species in DC activation by 
      whole cells and their representative microbe-associated molecular patterns 
      (MAMPs). MAMPs including lipoteichoic acid (LTA), lipoprotein (LPP), and 
      peptidoglycan (PGN) were purified from the two Bacillus species. Among the MAMPs, 
      LPP from both species most potently induced the maturation and activation of DCs 
      while PGN, but not LTA, moderately stimulated DCs. LPPs from both Bacillus 
      species enhanced the expression of DC maturation markers including CCR7, CD40, 
      CD80, CD83, CD86, CD205, MHC-I, and MHC-II. Among the MAMPs from B. cereus, PGN 
      most considerably lowered the endocytic capacity of DCs implying DC maturation 
      whereas PGN from B. subtilis lowered it to a similar degree to its LPP. 
      Furthermore, DCs sensitized with LPPs from both Bacillus species and PGN from B. 
      subtilis moderately induced TNF-alpha and IL-6 production. Notably, a combination of 
      MAMPs did not show any synergistic effect on DC activation. Taken together, our 
      results demonstrate that LPP is the key structural component in B. cereus and B. 
      subtilis that leads to DC activation.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Jeong, Sungho
AU  - Jeong S
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Im, Jintaek
AU  - Im J
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Lee, Dongwook
AU  - Lee D
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Ko, Kwang Hyun
AU  - Ko KH
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Yun, Cheol-Heui
AU  - Yun CH
AD  - Department of Agricultural Biotechnology, and Research Institute of Agriculture 
      and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
FAU - Han, Seung Hyun
AU  - Han SH
AD  - Department of Oral Microbiology and Immunology, and Dental Research Institute, 
      School of Dentistry, Seoul National University, Seoul 08826, Republic of Korea. 
      Electronic address: shhan-mi@snu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20231230
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Transcription Factors)
RN  - 0 (Lipoproteins)
RN  - 0 (Cytokines)
SB  - IM
MH  - *Bacillus/metabolism
MH  - Cell Differentiation
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Transcription Factors/metabolism
MH  - Dendritic Cells
MH  - Lipoproteins/metabolism
MH  - Cytokines/metabolism
OTO - NOTNLM
OT  - Bacillus cereus
OT  - Bacillus subtilis
OT  - Dendritic cells
OT  - Lipoproteins
COIS- Declaration of Competing Interest The authors declared that they have no 
      conflicts of interest to this work. We declare that we do not have any commercial 
      or associative interest that represents a conflict of interest in connection with 
      the work submitted.
EDAT- 2024/01/02 11:43
MHDA- 2024/01/12 06:43
CRDT- 2023/12/31 18:09
PHST- 2023/06/09 00:00 [received]
PHST- 2023/12/11 00:00 [revised]
PHST- 2023/12/22 00:00 [accepted]
PHST- 2024/01/12 06:43 [medline]
PHST- 2024/01/02 11:43 [pubmed]
PHST- 2023/12/31 18:09 [entrez]
AID - S0161-5890(23)00248-1 [pii]
AID - 10.1016/j.molimm.2023.12.009 [doi]
PST - ppublish
SO  - Mol Immunol. 2024 Jan;165:82-91. doi: 10.1016/j.molimm.2023.12.009. Epub 2023 Dec 
      30.