PMID- 38161029 OWN - NLM STAT- MEDLINE DCOM- 20240208 LR - 20240208 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) VI - 258 IP - Pt 2 DP - 2024 Feb TI - miR-2765 involved in ammonia nitrogen stress via negative regulation of autophagy in shrimp. PG - 129084 LID - S0141-8130(23)05983-4 [pii] LID - 10.1016/j.ijbiomac.2023.129084 [doi] AB - MicroRNA (miRNA) is a highly conserved non-coding tiny endogenous RNA molecule that regulates various cellular functions by inhibiting mRNA translation or promoting the degradation of proteins. In this study, we identified a specific miRNA (designed as Pva-miR-2765) from Penaeus vannamei, which widely distributed in different tissues of shrimp, with the highest concentration found in the intestine. Through fluorescence in situ hybridization (FISH), we observed that Pva-miR-2765 is primarily located in the cytoplasm. Interestingly, we found that the expression of Pva-miR-2765 significantly decreased in hemocytes, hepatopancreas and gill under ammonia nitrogen stress. Furthermore, when Pva-miR-2765 was silenced, the autophagy level in shrimp significantly increased. Additionally, Pva-miR-2765 was found to promote pathological damage in the hepatopancreas of shrimp. Subsequently, correlation analysis revealed a negative relationship between the expression of Pva-miR-2765 and PvTBC1D7. To confirm this interaction, we conducted a dual luciferase reporter gene assay, which demonstrated that Pva-miR-2765 inhibit the expression of PvTBC1D7 by interacting with its 3'UTR. And the expression level of PvTBC1D7 in shrimp decreased significantly under ammonia nitrogen stress in Pva-miR-2765 overexpressed. Our findings suggest that Pva-miR-2765 can reduce autophagy in P. vannamei by inhibiting the regulation of PvTBC1D7, thereby participating in the oxidative stress of shrimp caused by ammonia nitrogen stress. CI - Copyright (c) 2023 Elsevier B.V. All rights reserved. FAU - Wang, Feifei AU - Wang F AD - Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, PR China; Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China. Electronic address: ffwong0101@163.com. FAU - Zhao, Fei AU - Zhao F AD - Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, PR China. FAU - Deng, Yuting AU - Deng Y AD - Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, PR China. FAU - Tan, Aiping AU - Tan A AD - Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, PR China. FAU - Lai, Yingtiao AU - Lai Y AD - Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, PR China. FAU - Gong, Hua AU - Gong H AD - Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, PR China. FAU - Huang, Zhibin AU - Huang Z AD - Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, PR China. FAU - Liu, Yuan AU - Liu Y AD - Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China. FAU - Liang, Qingjian AU - Liang Q AD - Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China; Laboratory of Aquatic Animal Diseases and Immunity, School of Fishery, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: liang.qj@foxmail.com. FAU - Wang, Weina AU - Wang W AD - Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China. Electronic address: wangwn@scnu.edu.cn. LA - eng PT - Journal Article DEP - 20231229 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 RN - 7664-41-7 (Ammonia) RN - N762921K75 (Nitrogen) RN - 0 (MicroRNAs) SB - IM MH - Animals MH - Ammonia MH - In Situ Hybridization, Fluorescence MH - Nitrogen MH - *MicroRNAs MH - Autophagy MH - *Penaeidae OTO - NOTNLM OT - Ammonia nitrogen OT - Autophagy OT - Penaeus vannamei OT - PvTBC1D7 OT - Pva-miR-2765 COIS- Declaration of competing interest No potential conflict of interest was reported by the authors. EDAT- 2024/01/02 11:45 MHDA- 2024/02/08 06:43 CRDT- 2023/12/31 19:18 PHST- 2023/09/13 00:00 [received] PHST- 2023/11/27 00:00 [revised] PHST- 2023/12/18 00:00 [accepted] PHST- 2024/02/08 06:43 [medline] PHST- 2024/01/02 11:45 [pubmed] PHST- 2023/12/31 19:18 [entrez] AID - S0141-8130(23)05983-4 [pii] AID - 10.1016/j.ijbiomac.2023.129084 [doi] PST - ppublish SO - Int J Biol Macromol. 2024 Feb;258(Pt 2):129084. doi: 10.1016/j.ijbiomac.2023.129084. Epub 2023 Dec 29.