PMID- 38162872
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240103
IS  - 1658-3612 (Electronic)
IS  - 1658-3612 (Linking)
VI  - 18
IP  - 6
DP  - 2023 Dec
TI  - Effect of low frequency ultrasound waves on the morphology and viability of 
      cultured human gingival fibroblasts.
PG  - 1406-1416
LID - 10.1016/j.jtumed.2023.05.005 [doi]
AB  - OBJECTIVES: The aim of this study was to investigate the effect of the vibration 
      amplitude of mechanical ultrasound waves (27 kHz) on the viability and morphology 
      of human gingival fibroblasts (hGFs) when cultured on a biomaterial substrate. 
      METHOD: hGFs were seeded on tissue culture plates (TCPs) and an Ti6Al4V titanium 
      alloy surface in two groups for three days and seven days of cell culture. The 
      cells were subjected to three vibration amplitudes for 20 min each day. Scanning 
      electron microscope (SEM) images were used to characterize cell morphology. 
      RESULTS: Experiments showed that hGF cells became detached from their plates at a 
      vibration amplitude comparable to an intensity of 260 mW/cm2. In addition, hGfs 
      that received a vibrational amplitude comparable to an intensity of 50 mW/cm2 
      underwent significant proliferation proliferated significantly; however, cells 
      receiving higher amplitudes suffered from adverse effects. CONCLUSIONS: SEM 
      images of hGFs on titanium disks at vibration amplitude comparable to an 
      intensity 50 mW/cm2 showed a remarkable hexagonal architecture, which we refer to 
      as a honeycomb pattern. On day 6 the observed hGFs on TCPs, proliferated at a 
      higher rate and new cells attached uniformly on the existing layer of cells. 
      These data indicate the effect of cellular tissue as a substrate on the growth of 
      new hGFs under low-intensity ultrasound.
CI  - (c) 2023 The Authors.
FAU - Afshari, Mojtaba
AU  - Afshari M
AD  - Department of Manufacturing, Faculty of Mechanical Engineering, University of 
      Kashan, Kashan, Iran.
FAU - Amini, Saeid
AU  - Amini S
AD  - Department of Manufacturing, Faculty of Mechanical Engineering, University of 
      Kashan, Kashan, Iran.
FAU - Hashemibeni, Batool
AU  - Hashemibeni B
AD  - Torabinejad Dentistry Research Center and Department of Anatomical Sciences and 
      Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, 
      Isfahan, Iran.
AD  - Department of Anatomical Sciences, Medical School, Isfahan University of Medical 
      Sciences, Isfahan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20230518
PL  - Saudi Arabia
TA  - J Taibah Univ Med Sci
JT  - Journal of Taibah University Medical Sciences
JID - 101621911
PMC - PMC10757313
OTO - NOTNLM
OT  - Cell architecture
OT  - Honeycomb structure
OT  - Stress adaptation
OT  - Ultrasound device
EDAT- 2024/01/02 11:45
MHDA- 2024/01/02 11:46
PMCR- 2023/05/18
CRDT- 2024/01/01 04:30
PHST- 2023/01/21 00:00 [received]
PHST- 2023/03/13 00:00 [revised]
PHST- 2023/05/08 00:00 [accepted]
PHST- 2024/01/02 11:46 [medline]
PHST- 2024/01/02 11:45 [pubmed]
PHST- 2024/01/01 04:30 [entrez]
PHST- 2023/05/18 00:00 [pmc-release]
AID - S1658-3612(23)00072-0 [pii]
AID - 10.1016/j.jtumed.2023.05.005 [doi]
PST - epublish
SO  - J Taibah Univ Med Sci. 2023 May 18;18(6):1406-1416. doi: 
      10.1016/j.jtumed.2023.05.005. eCollection 2023 Dec.