PMID- 38165020
OWN - NLM
STAT- MEDLINE
DCOM- 20240311
LR  - 20240311
IS  - 1522-7278 (Electronic)
IS  - 1520-4081 (Linking)
VI  - 39
IP  - 4
DP  - 2024 Apr
TI  - A transcriptomics-based investigation of the mechanism of pulmonary fibrosis 
      induced by nickel oxide nanoparticles.
PG  - 2374-2389
LID - 10.1002/tox.24088 [doi]
AB  - Nickel oxide nanoparticles (NiONPs) are an emerging nanomaterial, which poses a 
      huge threat to the health of workplace population. Nanoparticles induce pulmonary 
      fibrosis, and its mechanisms are associated with noncoding RNAs (ncRNAs). 
      However, ncRNAs and competing endogenous RNA (ceRNA) networks which involved in 
      NiONP-induced pulmonary fibrosis are still unclear. This study aimed to identify 
      ncRNA-related ceRNA networks and investigate the role of the Wnt/beta-catenin 
      pathway in pulmonary fibrosis. Male Wistar rats were intratracheally instilled 
      with 0.015, 0.06, and 0.24 mg/kg NiONPs twice a week for 9 weeks. First, we found 
      there were 93 circularRNAs (circRNAs), 74 microRNAs (miRNAs), 124 long non-coding 
      RNAs (lncRNAs), and 1675 messenger RNAs (mRNAs) differentially expressed through 
      microarray analysis. Second, we constructed ceRNA networks among 
      lncRNAs/circRNAs, miRNAs and mRNAs and identified two ceRNA networks 
      (lncMelttl16/miR-382-5p/Hsd17b7 and circIqch/miR-181d-5p/Stat1) after real 
      time-quantitative polymerase chain reaction (RT-qPCR) validation. Furthermore, 
      based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) 
      analyses, ncRNAs were found to be involved in biological processes and signaling 
      pathways related to pulmonary fibrosis. KEGG analysis showed that NiONPs 
      activated the Wnt/beta-catenin pathway in rats. In vitro, HFL1 cells were treated 
      with 0, 50, 100, and 200 mug/mL NiONPs for 24 h. We found that NiONPs induced 
      collagen deposition and Wnt/beta-catenin pathway activation. Moreover, a blockade of 
      Wnt/beta-catenin pathway alleviated NiONP-induced collagen deposition. In 
      conclusion, these observations suggested that ncRNAs were crucial in pulmonary 
      fibrosis development and that the Wnt/beta-catenin pathway mediated the deposition 
      of collagen.
CI  - (c) 2024 Wiley Periodicals LLC.
FAU - Liu, Han
AU  - Liu H
AD  - Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 
      China.
FAU - Yang, Mengmeng
AU  - Yang M
AD  - Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 
      China.
FAU - Li, Kun
AU  - Li K
AUID- ORCID: 0000-0003-4374-7705
AD  - Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 
      China.
FAU - Gao, Qing
AU  - Gao Q
AD  - Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 
      China.
FAU - Zheng, Jinfa
AU  - Zheng J
AD  - Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 
      China.
FAU - Gong, Xuefeng
AU  - Gong X
AD  - Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 
      China.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 
      China.
FAU - Sun, Yingbiao
AU  - Sun Y
AUID- ORCID: 0000-0003-3711-8486
AD  - Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 
      China.
FAU - Chang, Xuhong
AU  - Chang X
AUID- ORCID: 0000-0002-5313-0576
AD  - Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, 
      China.
LA  - eng
GR  - 81602874/National Outstanding Youth Science Fund Project of National Natural 
      Science Foundation of China/
GR  - 31872757/National Natural Science Foundation of China/
GR  - 2022-rc-5/Science and Technology Plan Project of Chengguan District in Lanzhou/
GR  - 2023-2-9/Science and Technology Plan Project in Lanzhou/
PT  - Journal Article
DEP - 20240102
PL  - United States
TA  - Environ Toxicol
JT  - Environmental toxicology
JID - 100885357
RN  - 0 (beta Catenin)
RN  - C3574QBZ3Y (nickel monoxide)
RN  - 0 (RNA, Circular)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 9007-34-5 (Collagen)
RN  - 7OV03QG267 (Nickel)
SB  - IM
MH  - Male
MH  - Rats
MH  - Animals
MH  - beta Catenin/metabolism
MH  - RNA, Circular
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *Pulmonary Fibrosis/chemically induced/genetics
MH  - Rats, Wistar
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Gene Expression Profiling
MH  - Wnt Signaling Pathway/genetics
MH  - *Nanoparticles/toxicity
MH  - Collagen
MH  - Gene Regulatory Networks
MH  - *Nickel
OTO - NOTNLM
OT  - NiONPs
OT  - Wnt/beta-catenin
OT  - ceRNA network
OT  - noncoding RNA
OT  - pulmonary fibrosis
EDAT- 2024/01/02 11:44
MHDA- 2024/03/11 06:42
CRDT- 2024/01/02 08:00
PHST- 2023/10/15 00:00 [revised]
PHST- 2022/07/11 00:00 [received]
PHST- 2023/12/01 00:00 [accepted]
PHST- 2024/03/11 06:42 [medline]
PHST- 2024/01/02 11:44 [pubmed]
PHST- 2024/01/02 08:00 [entrez]
AID - 10.1002/tox.24088 [doi]
PST - ppublish
SO  - Environ Toxicol. 2024 Apr;39(4):2374-2389. doi: 10.1002/tox.24088. Epub 2024 Jan 
      2.