PMID- 38165823
OWN - NLM
STAT- MEDLINE
DCOM- 20240118
LR  - 20240202
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 12
IP  - 3
DP  - 2024 Jan 17
TI  - Bio-conjugated carbon dots for the bimodal detection of prostate cancer 
      biomarkers via sandwich fluorescence and electrochemical immunoassays.
PG  - 742-751
LID - 10.1039/d3tb02090h [doi]
AB  - Bimodal detection facilitates the accurate and reliable detection of cancer 
      biomarkers, which can assist in the early diagnosis of cancer. Herein, S-doped 
      carbon dots (OCDs) with a size of 3 nm and blue emission were synthesized by the 
      hydrothermal treatment of onion extract. The S-doped carbon dots were 
      bioconjugated with an antibody (OCDs@(PSA)Ab(HRP)) to design a nanoprobe for the 
      detection of prostate specific antigen (PSA), an important serum based prostate 
      cancer biomarker. The detection probe enabled the biomodal assay of PSA via 
      fluorescence immunoassay (FIA) and electrochemical immunoassay (ECIA). In both 
      assays, polyethylenimine stabilized polyaniline nanoparticles (PNPs) were used as 
      the immobilization matrix, which played a major role in widening the linear range 
      of biosensors (0.1 to 100 ng ml(-1) for ECIA and 5 to 120 ng ml(-1) for FIA). 
      Paper-based and smartphone-integrated fluorescence immuno-array developed using 
      the OCDs@(PSA)Ab(HRP) detection probe provided cost-effective and rapid 
      detection, while the electrochemical immunoassay provided a high sensitivity (7.8 
      muA ng(-1) ml(-1) cm(-2)) and low detection limit (38 pg ml(-1)) for PSA 
      detection. The role of OCDs in enhancing the sensor performance was deciphered by 
      carrying out detailed electrochemical studies with HRP enzyme-loaded OCDs. The 
      biosensor was used to detect PSA in human blood serum samples and the results 
      were consistent with conventional techniques. Owing to its analytical properties 
      coupled with simplicity, cost-effectiveness, and portability, the bimodal sensor 
      system has potential for application in clinical analysis.
FAU - Korram, Jyoti
AU  - Korram J
AD  - Chemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. 
      stawde@barc.gov.in.
FAU - Anbalagan, Amarnath Chellachamy
AU  - Anbalagan AC
AD  - Chemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. 
      stawde@barc.gov.in.
FAU - Banerjee, Anannya
AU  - Banerjee A
AUID- ORCID: 0009-0000-6219-2755
AD  - Fuel Chemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, 
      India.
FAU - Sawant, Shilpa N
AU  - Sawant SN
AUID- ORCID: 0000-0003-2835-4575
AD  - Chemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. 
      stawde@barc.gov.in.
AD  - Homi Bhabha National Institute, Anushaktinagar, Mumbai 400094, India.
LA  - eng
PT  - Journal Article
DEP - 20240117
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
RN  - 0 (Biomarkers, Tumor)
RN  - EC 3.4.21.77 (Prostate-Specific Antigen)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Male
MH  - Humans
MH  - Biomarkers, Tumor
MH  - Prostate-Specific Antigen/analysis
MH  - Carbon
MH  - Prostate/chemistry
MH  - *Metal Nanoparticles/chemistry
MH  - *Prostatic Neoplasms/diagnosis
MH  - Immunoassay/methods
EDAT- 2024/01/03 09:43
MHDA- 2024/01/18 06:43
CRDT- 2024/01/02 13:12
PHST- 2024/01/18 06:43 [medline]
PHST- 2024/01/03 09:43 [pubmed]
PHST- 2024/01/02 13:12 [entrez]
AID - 10.1039/d3tb02090h [doi]
PST - epublish
SO  - J Mater Chem B. 2024 Jan 17;12(3):742-751. doi: 10.1039/d3tb02090h.