PMID- 38166400
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240216
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Print)
IS  - 1525-7797 (Linking)
VI  - 25
IP  - 2
DP  - 2024 Feb 12
TI  - Noncovalent Conjugation of OVA323 to ELP Micelles Increases Immune Response.
PG  - 1027-1037
LID - 10.1021/acs.biomac.3c01091 [doi]
AB  - Subunit vaccines would benefit from a safe particle-based adjuvant. Elastin-like 
      polypeptide (ELP)-based micelles are interesting candidate adjuvants due to their 
      well-defined size and easy modification with protein-based cargo. Coiled coils 
      can facilitate noncovalent modifications, while potentially enhancing antigen 
      delivery through interaction with cell membranes. ELP micelles comprise ELP 
      diblock copolymers that self-assemble above a critical micelle temperature. In 
      this study, an amphiphilic ELP was conjugated to peptide "K", which forms a 
      heterodimeric coiled-coil complex with peptide "E". Self-assembled "covalent" 
      micelles containing ELP-OVA323 (i.e., model antigen OVA323 conjugated to ELP), 
      "coiled-coil" micelles containing ELP-K/E-OVA323 and "hybrid" micelles containing 
      ELP-K and ELP-OVA323 were shown to be monodisperse and spherical. Dendritic cells 
      (DCs) were exposed to all micelle compositions, and T-cell proliferation was 
      investigated. The presence of ELP-K enhanced micelle uptake and subsequent DC 
      maturation, resulting in enhanced CD4(+) T-cell proliferation, which makes ELPs 
      with coiled coil-associated antigens a promising vaccine platform.
FAU - van Strien, Jolinde
AU  - van Strien J
AD  - Department of Supramolecular and Biomaterials Chemistry, Leiden Institute of 
      Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
FAU - Makurat, Max
AU  - Makurat M
AD  - Department of Supramolecular and Biomaterials Chemistry, Leiden Institute of 
      Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
FAU - Zeng, Ye
AU  - Zeng Y
AD  - Department of Supramolecular and Biomaterials Chemistry, Leiden Institute of 
      Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
FAU - Olsthoorn, Rene
AU  - Olsthoorn R
AD  - Department of Supramolecular and Biomaterials Chemistry, Leiden Institute of 
      Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
FAU - Schneider, Gregory F
AU  - Schneider GF
AUID- ORCID: 0000-0001-5018-3309
AD  - Department of Supramolecular and Biomaterials Chemistry, Leiden Institute of 
      Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
FAU - Slutter, Bram
AU  - Slutter B
AD  - Department of BioTherapeutics, LACDR, Leiden University, P.O. Box 9502, 2300 RA 
      Leiden, The Netherlands.
FAU - MacKay, J Andrew
AU  - MacKay JA
AUID- ORCID: 0000-0002-3626-1654
AD  - Department of Pharmacology and Pharmaceutical Sciences, Alfred E. Mann School of 
      Pharmacy and Pharmaceutical Sciences, University of Southern California, 1985 
      Zonal Avenue, Los Angeles, California 90089-9121, United States.
FAU - Jiskoot, Wim
AU  - Jiskoot W
AD  - Department of BioTherapeutics, LACDR, Leiden University, P.O. Box 9502, 2300 RA 
      Leiden, The Netherlands.
FAU - Kros, Alexander
AU  - Kros A
AUID- ORCID: 0000-0002-3983-3048
AD  - Department of Supramolecular and Biomaterials Chemistry, Leiden Institute of 
      Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20240103
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Micelles)
RN  - 0 (Elastin-Like Polypeptides)
RN  - 9007-58-3 (Elastin)
RN  - 0 (Peptides)
RN  - 0 (Antigens)
SB  - IM
MH  - *Micelles
MH  - *Elastin-Like Polypeptides
MH  - Elastin/chemistry
MH  - Peptides/chemistry
MH  - Antigens
MH  - Lymphocyte Activation
PMC - PMC10865353
COIS- The authors declare no competing financial interest.
EDAT- 2024/01/03 09:43
MHDA- 2024/02/12 15:42
PMCR- 2024/02/14
CRDT- 2024/01/03 08:55
PHST- 2024/02/12 15:42 [medline]
PHST- 2024/01/03 09:43 [pubmed]
PHST- 2024/01/03 08:55 [entrez]
PHST- 2024/02/14 00:00 [pmc-release]
AID - 10.1021/acs.biomac.3c01091 [doi]
PST - ppublish
SO  - Biomacromolecules. 2024 Feb 12;25(2):1027-1037. doi: 10.1021/acs.biomac.3c01091. 
      Epub 2024 Jan 3.