PMID- 38171127 OWN - NLM STAT- MEDLINE DCOM- 20240115 LR - 20240115 IS - 1095-8630 (Electronic) IS - 0301-4797 (Linking) VI - 351 DP - 2024 Feb TI - Elevated CO(2) and nitrogen addition enhance the symbiosis and functions of rhizosphere microorganisms under cadmium exposure. PG - 120012 LID - S0301-4797(23)02800-1 [pii] LID - 10.1016/j.jenvman.2023.120012 [doi] AB - Soil microbes are fundamental to ecosystem health and productivity. How soil microbial communities are influenced by elevated atmospheric carbon dioxide (eCO(2)) concentration and nitrogen (N) deposition under heavy metal pollution remains uncertain, despite global exposure of terrestrial ecosystems to eCO(2), high N deposition and heavy metal stress. Here, we conducted a four year's open-top chamber experiment to assess the effects of soil cadmium (Cd) treatment (10 kg hm(-2) year(-1)) alone and combined treatments of Cd with eCO(2) concentration (700 ppm) and/or N addition (100 kg hm(-2) year(-1)) on tree growth and rhizosphere microbial community. Relative to Cd treatment alone, eCO(2) concentration in Cd contaminated soil increased the complexity of microbial networks, including the number links, average degree and positive/negative ratios. The combined effect of eCO(2) and N addition in Cd contaminated soil not only increased the complexity of microbial networks, but also enhanced the abundance of microbial urealysis related UreC and nitrifying related amoA1 and amoA2, and the richness of arbuscular mycorrhiza fungi (AMF), thereby improving the symbiotic functions between microorganisms and plants. Results from correlation analysis and structural equation model (SEM) further demonstrated that eCO(2) concentration and N addition acted on functions and networks differently. Elevated CO(2) positively regulated microbial networks and functions through phosphorus (P) and Cd concentration in roots, while N addition affected microbial functions through soil available N and soil organic carbon (SOC) concentration and microbial network through soil Cd concentration. Overall, our findings highlight that eCO(2) concentration and N addition make microbial communities towards ecosystem health that may mitigate Cd stress, and provide new insights into the microbiology supporting phytoremediation for Cd contaminated sites in current and future global change scenarios. CI - Copyright (c) 2023. Published by Elsevier Ltd. FAU - Chen, Minghao AU - Chen M AD - Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Science, Guangzhou, 510650, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Zhou, Shuyidan AU - Zhou S AD - Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Science, Guangzhou, 510650, China. FAU - Xiang, Ping AU - Xiang P AD - Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Science, Guangzhou, 510650, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Wang, Yutao AU - Wang Y AD - Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education and Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China. FAU - Luo, Xianzhen AU - Luo X AD - Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Science, Guangzhou, 510650, China. FAU - Zhang, Xiaofeng AU - Zhang X AD - Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Science, Guangzhou, 510650, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Wen, Dazhi AU - Wen D AD - Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Science, Guangzhou, 510650, China; College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi, 341000, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: dzwen@scbg.ac.cn. LA - eng PT - Journal Article DEP - 20240102 PL - England TA - J Environ Manage JT - Journal of environmental management JID - 0401664 RN - 00BH33GNGH (Cadmium) RN - 142M471B3J (Carbon Dioxide) RN - N762921K75 (Nitrogen) RN - 7440-44-0 (Carbon) RN - 0 (Soil) RN - 0 (Metals, Heavy) SB - IM MH - Rhizosphere MH - Cadmium/chemistry MH - Symbiosis MH - Carbon Dioxide/analysis MH - Nitrogen/analysis MH - Carbon MH - Soil Microbiology MH - Soil/chemistry MH - *Metals, Heavy/analysis MH - *Microbiota OTO - NOTNLM OT - Cadmium pollution OT - Global change OT - Nutrient cycling OT - Phytoremediation OT - Rhizosphere symbiosis COIS- Declaration of competing interest The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2024/01/04 11:43 MHDA- 2024/01/15 12:42 CRDT- 2024/01/03 18:05 PHST- 2023/10/06 00:00 [received] PHST- 2023/12/30 00:00 [revised] PHST- 2023/12/30 00:00 [accepted] PHST- 2024/01/15 12:42 [medline] PHST- 2024/01/04 11:43 [pubmed] PHST- 2024/01/03 18:05 [entrez] AID - S0301-4797(23)02800-1 [pii] AID - 10.1016/j.jenvman.2023.120012 [doi] PST - ppublish SO - J Environ Manage. 2024 Feb;351:120012. doi: 10.1016/j.jenvman.2023.120012. Epub 2024 Jan 2.