PMID- 38171388
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240214
IS  - 1873-6971 (Electronic)
IS  - 0367-326X (Linking)
VI  - 173
DP  - 2024 Mar
TI  - Free radical scavenging polyphenols isolated from Phyllanthus niruri L. 
      ameliorates hyperglycemia via SIRT1 induction and GLUT4 translocation in in vitro 
      and in vivo models.
PG  - 105803
LID - S0367-326X(23)00378-7 [pii]
LID - 10.1016/j.fitote.2023.105803 [doi]
AB  - Type 2 diabetes milletus (T2DM) is a complex multifaceted disorder characterized 
      by insulin resistance in skeletal muscle. Phyllanthus niruri L. is well reported 
      sub-tropical therapeutically beneficial ayurvedic medicinal plant from 
      Euphorbiaceae family used in various body ailments such as metabolic disorder 
      including diabetes. The present study emphasizes on the therapeutic potential of 
      Phyllanthus niruri L. and its phytochemical(s) against insulin resistance 
      conditions and impaired antioxidant activity thereby aiding as an 
      anti-hyperglycemic agent in targeting T2DM. Three compounds were isolated from 
      the most active ethyl acetate fraction namely compound 1 as 
      1-O-galloyl-6-O-luteoyl-beta-D-glucoside, compound 2 as brevifolincarboxylic acid 
      and compound 3 as ricinoleic acid. Compounds 1 and 2, the two polyphenols 
      enhanced the uptake of glucose and inhibited ROS levels in palmitate induced 
      C2C12 myotubes. PNEAF showed the potent enhancement of glucose uptake in 
      palmitate-induced insulin resistance condition in C2C12 myotubes and significant 
      ROS inhibition was observed in skeletal muscle cell line. PNEAF treated IR C2C12 
      myotubes and STZ induced Wistar rats elevated SIRT1, PGC1-alpha signaling cascade 
      through phosphorylation of AMPK and GLUT4 translocation resulting in insulin 
      sensitization. Our study revealed an insight into the efficacy of marker 
      compounds isolated from P. niruri and its enriched ethyl acetate fraction as ROS 
      scavenging agent and helps in attenuating insulin resistance condition in C2C12 
      myotubes as well as in STZ induced Wistar rat by restoring glucose metabolism. 
      Overall, this study can provide prospects for the marker-assisted development of 
      P. niruri as a phytopharmaceutical drug for the insulin resistance related 
      diabetic complications.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Swargiary, Deepsikha
AU  - Swargiary D
AD  - Chemical Biology Lab-I, Institute of Advanced Study in Science and Technology 
      (IASST), Guwahati, Assam, India; Academy of Scientific and Innovative Research 
      (AcSIR), Ghaziabad, U.P, India.
FAU - Kashyap, Bhaswati
AU  - Kashyap B
AD  - Chemical Biology Lab-I, Institute of Advanced Study in Science and Technology 
      (IASST), Guwahati, Assam, India.
FAU - Sarma, Pranamika
AU  - Sarma P
AD  - Chemical Biology Lab-I, Institute of Advanced Study in Science and Technology 
      (IASST), Guwahati, Assam, India.
FAU - Ahmed, Semim Akhtar
AU  - Ahmed SA
AD  - Chemical Biology Lab-I, Institute of Advanced Study in Science and Technology 
      (IASST), Guwahati, Assam, India; Academy of Scientific and Innovative Research 
      (AcSIR), Ghaziabad, U.P, India.
FAU - Gurumayum, Shalini
AU  - Gurumayum S
AD  - Chemical Biology Lab-I, Institute of Advanced Study in Science and Technology 
      (IASST), Guwahati, Assam, India.
FAU - Barge, Sagar Ramrao
AU  - Barge SR
AD  - Chemical Biology Lab-I, Institute of Advanced Study in Science and Technology 
      (IASST), Guwahati, Assam, India.
FAU - Basumatary, Devi
AU  - Basumatary D
AD  - Chemical Biology Lab-I, Institute of Advanced Study in Science and Technology 
      (IASST), Guwahati, Assam, India.
FAU - Borah, Jagat C
AU  - Borah JC
AD  - Chemical Biology Lab-I, Institute of Advanced Study in Science and Technology 
      (IASST), Guwahati, Assam, India; Academy of Scientific and Innovative Research 
      (AcSIR), Ghaziabad, U.P, India. Electronic address: borahjc@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20240101
PL  - Netherlands
TA  - Fitoterapia
JT  - Fitoterapia
JID - 16930290R
RN  - 76845O8NMZ (ethyl acetate)
RN  - 0 (Polyphenols)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - 0 (Insulin)
RN  - 0 (Palmitates)
RN  - 0 (Acetates)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Insulin Resistance
MH  - *Diabetes Mellitus, Type 2/drug therapy
MH  - Polyphenols/pharmacology/metabolism
MH  - *Phyllanthus
MH  - Reactive Oxygen Species/metabolism
MH  - Sirtuin 1
MH  - Rats, Wistar
MH  - Molecular Structure
MH  - Muscle Fibers, Skeletal
MH  - Insulin/metabolism
MH  - *Hyperglycemia
MH  - Palmitates/metabolism
MH  - Muscle, Skeletal/metabolism
MH  - *Acetates
OTO - NOTNLM
OT  - Free fatty acid
OT  - Oxidative stress
OT  - Phyllanthus niruri
OT  - Phytopharmaceuticals
OT  - Reactive oxygen species
OT  - T2DM
COIS- Declaration of competing interest The authors have declared that there is no 
      conflict of interest exists.
EDAT- 2024/01/04 11:43
MHDA- 2024/02/11 16:42
CRDT- 2024/01/03 19:10
PHST- 2023/09/29 00:00 [received]
PHST- 2023/12/12 00:00 [revised]
PHST- 2023/12/28 00:00 [accepted]
PHST- 2024/02/11 16:42 [medline]
PHST- 2024/01/04 11:43 [pubmed]
PHST- 2024/01/03 19:10 [entrez]
AID - S0367-326X(23)00378-7 [pii]
AID - 10.1016/j.fitote.2023.105803 [doi]
PST - ppublish
SO  - Fitoterapia. 2024 Mar;173:105803. doi: 10.1016/j.fitote.2023.105803. Epub 2024 
      Jan 1.