PMID- 38172698
OWN - NLM
STAT- MEDLINE
DCOM- 20240110
LR  - 20240229
IS  - 1471-2172 (Electronic)
IS  - 1471-2172 (Linking)
VI  - 25
IP  - 1
DP  - 2024 Jan 3
TI  - PRMT2 silencing regulates macrophage polarization through activation of STAT1 or 
      inhibition of STAT6.
PG  - 1
LID - 10.1186/s12865-023-00593-w [doi]
LID - 1
AB  - BACKGROUND: Macrophages play significant roles in innate immune responses and are 
      heterogeneous cells that can be polarized into M1 or M2 phenotypes. PRMT2 is one 
      of the type I protein arginine methyltransferases involved in inflammation. 
      However, the role of PRMT2 in M1/M2 macrophage polarization remains unclear. Our 
      study revealed the effect and mechanism of PRMT2 in macrophage polarization. 
      METHODS: Bone marrow-derived macrophages (BMDMs) were polarized to M1 or M2 state 
      by LPS plus murine recombinant interferon-gamma (IFN-gamma) or interleukin-4 (IL-4). 
      Quantitative polymerase chain reaction (qPCR), western blot and flow cytometry 
      (FCM) assay were performed and analyzed markers and signaling pathways of 
      macrophage polarization. RESULTS: We found that PRMT2 was obviously upregulated 
      in LPS/IFN-gamma-induced M1 macrophages, but it was little changed in IL-4-induced M2 
      macrophages. Furthermore, PRMT2 konckdown increased the expression of M1 
      macrophages markers through activation of STAT1 and decreased the expression of 
      M2 macrophages markers through inhibition of STAT6. CONCLUSIONS: PRMT2 silencing 
      modulates macrophage polarization by activating STAT1 to promote M1 and 
      inhibiting STAT6 to attenuate the M2 state.
CI  - (c) 2023. The Author(s).
FAU - Liu, Ting
AU  - Liu T
AD  - Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University 
      School of Medicine, Shanghai, 200025, China.
FAU - Li, Yinjiao
AU  - Li Y
AD  - Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University 
      School of Medicine, Shanghai, 200025, China.
FAU - Xu, Muqiu
AU  - Xu M
AD  - Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University 
      School of Medicine, Shanghai, 200025, China.
FAU - Huang, Hongjun
AU  - Huang H
AD  - Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University 
      School of Medicine, Shanghai, 200025, China. hhj12469@rjh.com.cn.
FAU - Luo, Yan
AU  - Luo Y
AD  - Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University 
      School of Medicine, Shanghai, 200025, China. ly11087@rjh.com.cn.
LA  - eng
GR  - No. 82102259/Yinjiao Li/
GR  - No. 82101840/Hongjun Huang/
GR  - No. 81871101/Yan Luo/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20240103
PL  - England
TA  - BMC Immunol
JT  - BMC immunology
JID - 100966980
RN  - 82115-62-6 (Interferon-gamma)
RN  - 207137-56-2 (Interleukin-4)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (STAT6 Transcription Factor)
RN  - EC 2.1.1.319 (PRMT2 protein, mouse)
RN  - 0 (Stat1 protein, mouse)
RN  - 0 (Stat6 protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Inflammation/metabolism
MH  - Interferon-gamma/metabolism
MH  - *Interleukin-4/metabolism
MH  - *Lipopolysaccharides/pharmacology/metabolism
MH  - Macrophage Activation
MH  - Macrophages
MH  - Signal Transduction
MH  - STAT6 Transcription Factor/metabolism
PMC - PMC10765854
OTO - NOTNLM
OT  - M1/M2
OT  - Macrophage polarization
OT  - PRMT2
OT  - STAT1/STAT6
COIS- The authors declare no competing interests.
EDAT- 2024/01/04 11:43
MHDA- 2024/01/05 06:42
PMCR- 2024/01/03
CRDT- 2024/01/03 23:45
PHST- 2023/02/08 00:00 [received]
PHST- 2023/12/14 00:00 [accepted]
PHST- 2024/01/05 06:42 [medline]
PHST- 2024/01/04 11:43 [pubmed]
PHST- 2024/01/03 23:45 [entrez]
PHST- 2024/01/03 00:00 [pmc-release]
AID - 10.1186/s12865-023-00593-w [pii]
AID - 593 [pii]
AID - 10.1186/s12865-023-00593-w [doi]
PST - epublish
SO  - BMC Immunol. 2024 Jan 3;25(1):1. doi: 10.1186/s12865-023-00593-w.