PMID- 38173390
OWN - NLM
STAT- MEDLINE
DCOM- 20240117
LR  - 20240117
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 58
IP  - 2
DP  - 2024 Jan 16
TI  - Uncovering the Dynamics of Urease and Carbonic Anhydrase Genes in Ureolysis, 
      Carbon Dioxide Hydration, and Calcium Carbonate Precipitation.
PG  - 1199-1210
LID - 10.1021/acs.est.3c06617 [doi]
AB  - The hydration of CO(2) suffers from kinetic inefficiencies that make its natural 
      trapping impractically sluggish. However, CO(2)-fixing carbonic anhydrases (CAs) 
      remarkably accelerate its equilibration by 6 orders of magnitude and are, 
      therefore, "ideal" catalysts. Notably, CA has been detected in ureolytic 
      bacteria, suggesting its potential involvement in microbially induced carbonate 
      precipitation (MICP), yet the dynamics of the urease (Ur) and CA genes remain 
      poorly understood. Here, through the use of the ureolytic bacteriumSporosarcina 
      pasteurii, we investigate the differing role of Ur and CA in ureolysis, CO(2) 
      hydration, and CaCO(3) precipitation with increasing CO(2(g)) concentrations. We 
      show that Ur gene up-regulation coincides with an increase in [HCO(3)(-)] 
      following the hydration of CO(2) to HCO(3)(-) by CA. Hence, CA physiologically 
      promotes buffering, which enhances solubility trapping and affects the phase of 
      the CaCO(3) mineral formed. Understanding the role of CO(2) hydration on the 
      performance of ureolysis and CaCO(3) precipitation provides essential new 
      insights, required for the development of next-generation biocatalyzed CO(2) 
      trapping technologies.
FAU - Clara Saracho, Alexandra
AU  - Clara Saracho A
AUID- ORCID: 0000-0002-3900-8734
AD  - Department of Civil, Architectural and Environmental Engineering, The University 
      of Texas at Austin, 301 E Dean Keeton St C1700, Austin, Texas 78712, United 
      States.
FAU - Marek, Ewa J
AU  - Marek EJ
AUID- ORCID: 0000-0002-8318-2131
AD  - Department of Chemical Engineering and Biotechnology, University of Cambridge, 
      Philippa Fawcett Dr, Cambridge CB3 0AS, United Kingdom.
LA  - eng
PT  - Journal Article
DEP - 20240104
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 142M471B3J (Carbon Dioxide)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - EC 3.5.1.5 (Urease)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - 8W8T17847W (Urea)
SB  - IM
MH  - *Carbon Dioxide
MH  - Calcium Carbonate
MH  - Urease
MH  - *Carbonic Anhydrases/genetics
MH  - Urea
MH  - Chemical Precipitation
OTO - NOTNLM
OT  - CO2 sequestration
OT  - climate change
OT  - microbial metabolism
OT  - microbially induced calcium carbonate precipitation
EDAT- 2024/01/04 11:44
MHDA- 2024/01/17 06:43
CRDT- 2024/01/04 03:47
PHST- 2024/01/17 06:43 [medline]
PHST- 2024/01/04 11:44 [pubmed]
PHST- 2024/01/04 03:47 [entrez]
AID - 10.1021/acs.est.3c06617 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2024 Jan 16;58(2):1199-1210. doi: 10.1021/acs.est.3c06617. 
      Epub 2024 Jan 4.