PMID- 38175302 OWN - NLM STAT- MEDLINE DCOM- 20240105 LR - 20240205 IS - 1436-3771 (Electronic) IS - 1432-6981 (Linking) VI - 28 IP - 1 DP - 2024 Jan 4 TI - Saliva biofilm-derived outer membrane vesicles regulate biofilm formation and immune response of oral epithelial cells on titanium surfaces. PG - 75 LID - 10.1007/s00784-023-05454-9 [doi] AB - OBJECTIVES: While the significant roles of outer membrane vesicles (OMVs) from individual oral bacterial species in bacterial-host interactions are known, the involvement of saliva biofilm-derived OMVs in peri-implant disease pathogenesis remains unclear. This study aimed to investigate the effect of saliva biofilm-derived OMVs on regulating saliva biofilm formation and modulating the immune response of the epithelial cells on titanium surfaces. MATERIALS AND METHODS: Saliva derived biofilms were cultured on tissue culture plates (TCP) for 4 days using pooled saliva from four healthy donors. OMVs secreted from the TCP bound biofilm (referred to as OMVs or healthy saliva biofilm OMVs) were enriched using the size-exclusion chromatography method. We then evaluated the effects of these OMVs on the viability, metabolic activity, and the presence of oral pathogens in saliva biofilm grown on titanium discs for 24 h and 72 h. Furthermore, the impact of OMVs on the mRNA expression and inflammatory cytokines [interleukin (IL)-6, IL-1alpha, and monocyte chemoattractant protein-1 (MCP-1)] in human oral epithelial cells (OKF6/TERT-2) was investigated using RT-qPCR and enzyme-linked immunosorbent assay (ELISA), respectively. RESULTS: Healthy saliva biofilm OMVs improved the biomass and activity of saliva biofilm cultured on the titanium surfaces, with inhibited Porphyromonas gingivalis and Fusobacterium nucleatum, and enhanced Streptococcus mutans expression. Additionally, OMVs increased pro-inflammatory cytokine IL-6 mRNA and IL-6 cytokine expression in human oral epithelial cells. However, IL-1alpha and MCP-1 cytokines were inhibited 24-hour post-incubation with OMVs. CONCLUSION: Healthy saliva biofilm derived OMVs regulate the activity and pathogen composition of biofilms formed on titanium, while modulating the secretion of pro-inflammation factors of oral epithelial cells grown on titanium surfaces. CLINICAL RELEVANCE: Healthy saliva biofilm OMVs may regulate the early biofilm formation on abutment surfaces and modulate epithelial cell immune response, which may alter the peri-implant niche and participate in the pathogenesis of peri-implant disease. CI - (c) 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Huang, Baoxin AU - Huang B AD - Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, People's Republic of China. AD - Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, People's Republic of China. AD - The University of Queensland, School of Dentistry, QLD, Brisbane, 4006, Australia. AD - The University of Queensland, School of Dentistry, Centre for Oral-Facial Regeneration, Rehabilitation and Reconstruction (COR3), Brisbane, Queensland, Australia. FAU - Liu, Chun AU - Liu C AD - The University of Queensland, School of Dentistry, QLD, Brisbane, 4006, Australia. AD - The University of Queensland, School of Dentistry, Centre for Oral-Facial Regeneration, Rehabilitation and Reconstruction (COR3), Brisbane, Queensland, Australia. FAU - Yang, Jieting AU - Yang J AD - Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, People's Republic of China. AD - Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, People's Republic of China. FAU - Xiang, Enmao AU - Xiang E AD - The University of Queensland, School of Dentistry, QLD, Brisbane, 4006, Australia. AD - The University of Queensland, School of Dentistry, Centre for Oral-Facial Regeneration, Rehabilitation and Reconstruction (COR3), Brisbane, Queensland, Australia. FAU - Ivanovski, Saso AU - Ivanovski S AD - The University of Queensland, School of Dentistry, QLD, Brisbane, 4006, Australia. s.ivanovski@uq.edu.au. AD - The University of Queensland, School of Dentistry, Centre for Oral-Facial Regeneration, Rehabilitation and Reconstruction (COR3), Brisbane, Queensland, Australia. s.ivanovski@uq.edu.au. FAU - Han, Pingping AU - Han P AD - The University of Queensland, School of Dentistry, QLD, Brisbane, 4006, Australia. p.han@uq.edu.au. AD - The University of Queensland, School of Dentistry, Centre for Oral-Facial Regeneration, Rehabilitation and Reconstruction (COR3), Brisbane, Queensland, Australia. p.han@uq.edu.au. LA - eng PT - Journal Article DEP - 20240104 PL - Germany TA - Clin Oral Investig JT - Clinical oral investigations JID - 9707115 RN - 0 (Interleukin-6) RN - D1JT611TNE (Titanium) RN - 0 (Cytokines) RN - 0 (RNA, Messenger) MH - Humans MH - *Saliva MH - Interleukin-6 MH - Titanium/pharmacology MH - *Peri-Implantitis MH - Cytokines MH - Biofilms MH - Epithelial Cells MH - Immunity MH - RNA, Messenger OTO - NOTNLM OT - Dental implants OT - Epithelial cells OT - Outer membrane vesicles OT - Saliva biofilm OT - Titanium EDAT- 2024/01/04 12:41 MHDA- 2024/01/05 06:42 CRDT- 2024/01/04 11:11 PHST- 2023/08/12 00:00 [received] PHST- 2023/12/18 00:00 [accepted] PHST- 2024/01/05 06:42 [medline] PHST- 2024/01/04 12:41 [pubmed] PHST- 2024/01/04 11:11 [entrez] AID - 10.1007/s00784-023-05454-9 [pii] AID - 10.1007/s00784-023-05454-9 [doi] PST - epublish SO - Clin Oral Investig. 2024 Jan 4;28(1):75. doi: 10.1007/s00784-023-05454-9.