PMID- 38180327
OWN - NLM
STAT- Publisher
LR  - 20240105
IS  - 1899-5276 (Print)
IS  - 1899-5276 (Linking)
DP  - 2024 Jan 5
TI  - MCT1 gene silencing enhances the immune effect of dendritic cells on cervical 
      cancer cells.
LID - 10.17219/acem/171446 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are a key class of immune cells that migrate to 
      the draining lymph nodes and present processed antigenic peptides to lymphocytes 
      after being activated by external stimuli, thereby establishing adaptive 
      immunity. Moreover, DCs play an important role in tumor immunity. OBJECTIVES: The 
      aim of the study was to investigate whether MCT1 gene silencing in DCs affects 
      their ability to mount an immune response against cervical cancer cells. MATERIAL 
      AND METHODS: We silenced the expression of MCT1 in DCs from mouse bone marrow 
      (BM) by infection with adenovirus. The surface antigen profile of DCs was 
      analyzed by flow cytometry and cytokine secretion was evaluated using 
      enzyme-linked immunosorbent assay (ELISA) following sodium lactate (sLA) exposure 
      and lipopolysaccharide (LPS) stimulation. Then, various groups of DC-induced 
      cytotoxic T lymphocytes (CTLs) were prepared and their cytotoxicity against U14 
      was tested. RESULTS: Without sLA exposure, silencing MCT1 did not affect the 
      expression of CD1a, CD80, CD83, CD86, and major histocompatibility complex class 
      II (MHCII) in DCs after LPS challenge. Similar results were found for interleukin 
      (IL)-6, IL-12 p70 and tumor necrosis factor alpha (TNF-alpha). After sLA exposure, 
      silencing MCT1 significantly decreased the expression of CD1a, CD80, CD83, CD86, 
      and MHCII in DCs after the LPS challenge, as well as the secretion of IL-6, IL-12 
      p70 and TNF-alpha. In addition, sLA exposure significantly reduced the toxicity and 
      inhibited the proliferation of DC-induced CTLs compared to U14 cells in vitro and 
      in vivo. However, silencing MCT1 significantly attenuated the changes caused by 
      sLA exposure. At the same time, in the absence of sLA, silencing MCT1 did not 
      affect the toxicity nor inhibit the proliferation of DC-induced CTLs on U14 
      cells. CONCLUSIONS: Lactate exposure reduces the immune effect of DCs on cervical 
      cancer cells, but MCT1 gene silencing attenuates these alterations.
FAU - Sui, Xiaoxin
AU  - Sui X
AD  - Department of Obstetrics and Gynecology, Shanghai Changzheng Hospital, Naval 
      Medical University, Shanghai, China.
FAU - Xi, Xiaowei
AU  - Xi X
AD  - Department of Obstetrics and Gynecology, General Hospital, Shanghai Jiao Tong 
      University School of Medicine, China.
LA  - eng
PT  - Journal Article
DEP - 20240105
PL  - Poland
TA  - Adv Clin Exp Med
JT  - Advances in clinical and experimental medicine : official organ Wroclaw Medical 
      University
JID - 101138582
SB  - IM
OTO - NOTNLM
OT  - MCT1
OT  - cervical cancer
OT  - dendritic cells
OT  - immunity
EDAT- 2024/01/05 12:42
MHDA- 2024/01/05 12:42
CRDT- 2024/01/05 09:58
PHST- 2022/08/29 00:00 [received]
PHST- 2023/03/01 00:00 [revised]
PHST- 2023/08/21 00:00 [accepted]
PHST- 2024/01/05 12:42 [medline]
PHST- 2024/01/05 12:42 [pubmed]
PHST- 2024/01/05 09:58 [entrez]
AID - 10.17219/acem/171446 [doi]
PST - aheadofprint
SO  - Adv Clin Exp Med. 2024 Jan 5. doi: 10.17219/acem/171446.