PMID- 38187760
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240210
DP  - 2023 Dec 23
TI  - Dual carbon sequestration with photosynthetic living materials.
LID - 2023.12.22.572991 [pii]
LID - 10.1101/2023.12.22.572991 [doi]
AB  - Natural ecosystems offer efficient pathways for carbon sequestration, serving as 
      a resilient approach to remove CO(2) from the atmosphere with minimal 
      environmental impact. However, the control of living systems outside of their 
      native environments is often challenging. Here, we engineered a photosynthetic 
      living material for dual CO(2) sequestration by immobilizing photosynthetic 
      microorganisms within a printable polymeric network. The carbon concentrating 
      mechanism of the cyanobacteria enabled accumulation of CO(2) within the cell, 
      resulting in biomass production. Additionally, the metabolic production of OH(-) 
      ions in the surrounding medium created an environment for the formation of 
      insoluble carbonates via microbially-induced calcium carbonate precipitation 
      (MICP). Digital design and fabrication of the living material ensured sufficient 
      access to light and nutrient transport of the encapsulated cyanobacteria, which 
      were essential for long-term viability (more than one year) as well as efficient 
      photosynthesis and carbon sequestration. The photosynthetic living materials 
      sequestered approximately 2.5 mg of CO(2) per gram of hydrogel material over 30 
      days via dual carbon sequestration, with 2.2 +/- 0.9 mg stored as insoluble 
      carbonates. Over an extended incubation period of 400 days, the living materials 
      sequestered 26 +/- 7 mg of CO(2) per gram of hydrogel material in the form of 
      stable minerals. These findings highlight the potential of photosynthetic living 
      materials for scalable carbon sequestration, carbon-neutral infrastructure, and 
      green building materials. The simplicity of maintenance, coupled with its 
      scalability nature, suggests broad applications of photosynthetic living 
      materials as a complementary strategy to mitigate CO(2) emissions.
FAU - Dranseike, Dalia
AU  - Dranseike D
AUID- ORCID: 0000-0002-5935-3666
AD  - Macromolecular Engineering Laboratory, Department of Mechanical and Process 
      Engineering, ETH Zurich, Zurich, CH.
FAU - Cui, Yifan
AU  - Cui Y
AUID- ORCID: 0000-0002-3772-4876
AD  - Macromolecular Engineering Laboratory, Department of Mechanical and Process 
      Engineering, ETH Zurich, Zurich, CH.
FAU - Ling, Andrea S
AU  - Ling AS
AUID- ORCID: 0000-0003-2383-4473
AD  - Digital Building Technologies, Department of Architecture, ETH Zurich, Zurich, 
      CH.
FAU - Donat, Felix
AU  - Donat F
AUID- ORCID: 0000-0002-3940-9183
AD  - Laboratory of Energy Science and Engineering, Department of Mechanical and 
      Process Engineering, ETH Zurich, Zurich, CH.
FAU - Bernhard, Stephane
AU  - Bernhard S
AUID- ORCID: 0000-0002-6534-6905
AD  - Macromolecular Engineering Laboratory, Department of Mechanical and Process 
      Engineering, ETH Zurich, Zurich, CH.
FAU - Bernero, Margherita
AU  - Bernero M
AUID- ORCID: 0009-0000-2327-3544
AD  - Institute for Biomechanics, Department of Health Sciences and Technology, ETH 
      Zurich, Zurich, CH.
FAU - Areeckal, Akhil
AU  - Areeckal A
AD  - Macromolecular Engineering Laboratory, Department of Mechanical and Process 
      Engineering, ETH Zurich, Zurich, CH.
FAU - Qin, Xiao-Hua
AU  - Qin XH
AUID- ORCID: 0000-0001-8355-3230
AD  - Institute for Biomechanics, Department of Health Sciences and Technology, ETH 
      Zurich, Zurich, CH.
FAU - Oakey, John S
AU  - Oakey JS
AUID- ORCID: 0000-0003-1023-1964
AD  - Department of Chemical and Biomedical Engineering, University of Wyoming, 
      Laramie, Wyoming, US.
FAU - Dillenburger, Benjamin
AU  - Dillenburger B
AUID- ORCID: 0000-0002-5153-2985
AD  - Digital Building Technologies, Department of Architecture, ETH Zurich, Zurich, 
      CH.
FAU - Studart, Andre R
AU  - Studart AR
AUID- ORCID: 0000-0003-4205-8545
AD  - Complex Materials, Department of Materials, ETH Zurich, Zurich, CH.
FAU - Tibbitt, Mark W
AU  - Tibbitt MW
AUID- ORCID: 0000-0002-4917-7187
AD  - Macromolecular Engineering Laboratory, Department of Mechanical and Process 
      Engineering, ETH Zurich, Zurich, CH.
LA  - eng
GR  - P20 GM103432/GM/NIGMS NIH HHS/United States
PT  - Preprint
DEP - 20231223
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
PMC - PMC10769394
OTO - NOTNLM
OT  - 3D printing
OT  - CO2 sequestration
OT  - living materials
EDAT- 2024/01/08 06:42
MHDA- 2024/01/08 06:43
PMCR- 2024/01/05
CRDT- 2024/01/08 04:54
PHST- 2024/01/08 06:42 [pubmed]
PHST- 2024/01/08 06:43 [medline]
PHST- 2024/01/08 04:54 [entrez]
PHST- 2024/01/05 00:00 [pmc-release]
AID - 2023.12.22.572991 [pii]
AID - 10.1101/2023.12.22.572991 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2023 Dec 23:2023.12.22.572991. doi: 
      10.1101/2023.12.22.572991.