PMID- 38193984 OWN - NLM STAT- Publisher LR - 20240109 IS - 1559-1182 (Electronic) IS - 0893-7648 (Linking) DP - 2024 Jan 9 TI - m(6)A-Mediated Upregulation of lncRNA CHASERR Promotes the Progression of Glioma by Modulating the miR-6893-3p/TRIM14 Axis. LID - 10.1007/s12035-023-03911-w [doi] AB - Long noncoding RNAs (lncRNAs) play crucial roles in tumor progression and are dysregulated in glioma. However, the functional roles of lncRNAs in glioma remain largely unknown. In this study, we utilized the TCGA (the Cancer Genome Atlas database) and GEPIA2 (Gene Expression Profiling Interactive Analysis 2) databases and observed the overexpression of lncRNA CHASERR in glioma tissues. We subsequently investigated this phenomenon in glioma cell lines. The effects of lncRNA CHASERR on glioma proliferation, migration, and invasion were analyzed using in vitro and in vivo experiments. Additionally, the regulatory mechanisms among PTEN/p-Akt/mTOR and Wnt/beta-catenin, lncRNA CHASERR, Micro-RNA-6893-3p(miR-6893-3p), and tripartite motif containing14 (TRIM14) were investigated via bioinformatics analyses, quantitative real-time PCR (qRT-PCR), western blot (WB), RNA immunoprecipitation (RIP), dual luciferase reporter assay, fluorescence in situ hybridization (FISH), and RNA sequencing assays. RIP and RT-qRCR were used to analyze the regulatory effect of N(6)-methyladenosine(m(6)A) on the aberrantly expressed lncRNA CHASERR. High lncRNA CHASERR expression was observed in glioma tissues and was associated with unfavorable prognosis in glioma patients. Further functional assays showed that lncRNA CHASERR regulates glioma growth and metastasis in vitro and in vivo. Mechanistically, lncRNA CHASERR sponged miR-6893-3p to upregulate TRIM14 expression, thereby facilitating glioma progression. Additionally, the activation of PTEN/p-Akt/mTOR and Wnt/beta-catenin pathways by lncRNA CHASERR, miR-6893-3p, and TRIM14 was found to regulate glioma progression. Moreover, the upregulation of lncRNA CHASERR was observed in response to N6-methyladenosine modification, which was facilitated by METTL3/YTHDF1-mediated RNA transcripts. This study elucidates the m6A/lncRNACHASERR/miR-6893-3p/TRIM14 pathway that contributes to glioma progression and underscores the potential of lncRNA CHASERR as a novel prognostic indicator and therapeutic target for glioma. CI - (c) 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Wu, Xingwei AU - Wu X AD - Anhui Province Key Laboratory of Non-Coding RNA Basic Research and Clinical Transformation, Wannan Medical College, Wuhu, 241001, China. AD - Key Laboratory of Non-Coding RNA Transformation Research of Anhui Higher Education Institution (Yijishan Hospital of Wannan Medical College), Wuhu, 241001, Anhui, China. AD - Central Laboratory, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China. FAU - Fu, Minjie AU - Fu M AD - Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, China. AD - Shanghai Key Laboratory of Brain Function Restoration and Neural Regeneration, Neurosurgical Institute of Fudan University, Shanghai, China. FAU - Ge, Chang AU - Ge C AD - Department of Psychology, Zhejiang Sci-Tech University, Hangzhou, 310000, Zhejiang, China. FAU - Zhou, Hanyu AU - Zhou H AD - Anhui Province Key Laboratory of Non-Coding RNA Basic Research and Clinical Transformation, Wannan Medical College, Wuhu, 241001, China. AD - Key Laboratory of Non-Coding RNA Transformation Research of Anhui Higher Education Institution (Yijishan Hospital of Wannan Medical College), Wuhu, 241001, Anhui, China. AD - Central Laboratory, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China. AD - Department of Psychology, Zhejiang Sci-Tech University, Hangzhou, 310000, Zhejiang, China. FAU - Huang, Haoyu AU - Huang H AD - Anhui Province Key Laboratory of Non-Coding RNA Basic Research and Clinical Transformation, Wannan Medical College, Wuhu, 241001, China. AD - Key Laboratory of Non-Coding RNA Transformation Research of Anhui Higher Education Institution (Yijishan Hospital of Wannan Medical College), Wuhu, 241001, Anhui, China. AD - Central Laboratory, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China. FAU - Zhong, Min AU - Zhong M AD - Anhui Province Key Laboratory of Non-Coding RNA Basic Research and Clinical Transformation, Wannan Medical College, Wuhu, 241001, China. AD - Key Laboratory of Non-Coding RNA Transformation Research of Anhui Higher Education Institution (Yijishan Hospital of Wannan Medical College), Wuhu, 241001, Anhui, China. AD - Central Laboratory, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China. AD - Department of Psychology, Zhejiang Sci-Tech University, Hangzhou, 310000, Zhejiang, China. FAU - Zhang, Mengying AU - Zhang M AD - Anhui Province Key Laboratory of Non-Coding RNA Basic Research and Clinical Transformation, Wannan Medical College, Wuhu, 241001, China. AD - Key Laboratory of Non-Coding RNA Transformation Research of Anhui Higher Education Institution (Yijishan Hospital of Wannan Medical College), Wuhu, 241001, Anhui, China. AD - Central Laboratory, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China. AD - Department of Psychology, Zhejiang Sci-Tech University, Hangzhou, 310000, Zhejiang, China. FAU - Xu, Hao AU - Xu H AD - Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, China. FAU - Zhu, Guoping AU - Zhu G AD - Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, 241001, Anhui, China. gpz2012@ahnu.edu.cn. AD - College of Life Sciences, Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education Institutes, Anhui Normal University, Wuhu, 241001, Anhui, China. gpz2012@ahnu.edu.cn. AD - Auhui Provincial Engineering Research Centre for Molecular Detection and Diagnostics, College of Life Sciences, Anhui Normal University, Wuhu, 241001, Anhui, China. gpz2012@ahnu.edu.cn. FAU - Hua, Wei AU - Hua W AD - Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 200040, China. hs_huawei@126.com. AD - Shanghai Key Laboratory of Brain Function Restoration and Neural Regeneration, Neurosurgical Institute of Fudan University, Shanghai, China. hs_huawei@126.com. FAU - Lv, Kun AU - Lv K AD - Anhui Province Key Laboratory of Non-Coding RNA Basic Research and Clinical Transformation, Wannan Medical College, Wuhu, 241001, China. lvkun315@126.com. AD - Key Laboratory of Non-Coding RNA Transformation Research of Anhui Higher Education Institution (Yijishan Hospital of Wannan Medical College), Wuhu, 241001, Anhui, China. lvkun315@126.com. AD - Central Laboratory, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China. lvkun315@126.com. AD - Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, 241001, Anhui, China. lvkun315@126.com. AD - College of Life Sciences, Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education Institutes, Anhui Normal University, Wuhu, 241001, Anhui, China. lvkun315@126.com. AD - Auhui Provincial Engineering Research Centre for Molecular Detection and Diagnostics, College of Life Sciences, Anhui Normal University, Wuhu, 241001, Anhui, China. lvkun315@126.com. AD - Clinical Research Center for Critical Respiratory Medicine of Anhui Province, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China. lvkun315@126.com. FAU - Yang, Hui AU - Yang H AUID- ORCID: 0000-0002-5307-3970 AD - Anhui Province Key Laboratory of Non-Coding RNA Basic Research and Clinical Transformation, Wannan Medical College, Wuhu, 241001, China. yanghui0203@wnmc.edu.cn. AD - Key Laboratory of Non-Coding RNA Transformation Research of Anhui Higher Education Institution (Yijishan Hospital of Wannan Medical College), Wuhu, 241001, Anhui, China. yanghui0203@wnmc.edu.cn. AD - Central Laboratory, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China. yanghui0203@wnmc.edu.cn. AD - Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, 241001, Anhui, China. yanghui0203@wnmc.edu.cn. AD - College of Life Sciences, Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education Institutes, Anhui Normal University, Wuhu, 241001, Anhui, China. yanghui0203@wnmc.edu.cn. AD - Auhui Provincial Engineering Research Centre for Molecular Detection and Diagnostics, College of Life Sciences, Anhui Normal University, Wuhu, 241001, Anhui, China. yanghui0203@wnmc.edu.cn. AD - Clinical Research Center for Critical Respiratory Medicine of Anhui Province, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241001, Anhui, China. yanghui0203@wnmc.edu.cn. LA - eng GR - 81802503/National Nature Science Foundation of China/ GR - 81772180/National Nature Science Foundation of China/ GR - 82072370/National Nature Science Foundation of China/ PT - Journal Article DEP - 20240109 PL - United States TA - Mol Neurobiol JT - Molecular neurobiology JID - 8900963 SB - IM OTO - NOTNLM OT - Glioma OT - N 6-methyladenosine OT - TRIM14 OT - lncRNA CHASERR OT - miR-6893-3p EDAT- 2024/01/09 13:42 MHDA- 2024/01/09 13:42 CRDT- 2024/01/09 11:06 PHST- 2023/08/03 00:00 [received] PHST- 2023/12/28 00:00 [accepted] PHST- 2024/01/09 13:42 [medline] PHST- 2024/01/09 13:42 [pubmed] PHST- 2024/01/09 11:06 [entrez] AID - 10.1007/s12035-023-03911-w [pii] AID - 10.1007/s12035-023-03911-w [doi] PST - aheadofprint SO - Mol Neurobiol. 2024 Jan 9. doi: 10.1007/s12035-023-03911-w.