PMID- 38194130
OWN - NLM
STAT- MEDLINE
DCOM- 20240116
LR  - 20240116
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 51
IP  - 1
DP  - 2024 Jan 9
TI  - Mesenchymal stem cells (MSCs) from the mouse bone marrow show differential 
      expression of interferon regulatory factors IRF-1 and IRF-2.
PG  - 97
LID - 10.1007/s11033-023-09025-9 [doi]
AB  - BACKGROUND: Interferon regulatory factors (IRF-1 and IRF-2) are transcription 
      factors widely implicated in various cellular processes, including regulation of 
      inflammatory responses to pathogens, cell proliferation, oncogenesis, 
      differentiation, autophagy, and apoptosis. METHODS: We have studied the 
      expression of IRF-1, IRF-2 mRNAs by RT-PCR, cellular localization of the proteins 
      by immunofluorescence, and expression of mRNAs of genes regulated by IRF-1, IRF-2 
      by RT-PCR in mouse bone marrow cells (BMCs) and mesenchymal stem cells (MSCs). 
      RESULTS: Higher level of IRF-1 mRNA was observed in BMCs and MSCs compared to 
      that of IRF-2. Similarly, differential expression of IRF-1 and IRF-2 proteins was 
      observed in BMCs and MSCs. IRF-1 was predominantly localized in the cytoplasm, 
      whereas IRF-2 was localized in the nuclei of BMCs. MSCs showed nucleo-cytoplasmic 
      distribution of IRF-1 and nuclear localization of IRF-2. Constitutive expression 
      of IRF-1 and IRF-2 target genes: monocyte chemoattractant protein-1 (MCP-1), 
      vascular cell adhesion molecule-1 (VCAM-1), cyclooxygenase-2 (COX-2), matrix 
      metalloproteinase-9 (MMP-9), and caspase-1 was observed in both BMCs and MSCs. 
      MSCs showed constitutive expression of the pluripotency-associated factors, 
      Oct3/4 and Sox-2. Lipopolysaccharide (LPS)-treatment of MSCs induced prominent 
      cellular localization of IRF-1 and IRF-2. CONCLUSIONS: Our results suggest that 
      IRF-1 and IRF-2 exhibit differential expression of their mRNAs and subcellular 
      localization of the proteins in BMCs and MSCs. These cells also show differential 
      levels of constitutive expression of IRF-1 and IRF-2 target genes. This may 
      regulate immune-responsive properties of BMCs and MSCs through IRF-1, 
      IRF-2-dependent gene expression and protein-protein interaction. Regulating IRF-1 
      and IRF-2 may be helpful for immunomodulatory functions of MSCs for cell therapy 
      and regenerative medicine.
CI  - (c) 2024. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Chaudhary, Jitendra Kumar
AU  - Chaudhary JK
AD  - Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru 
      University, New Delhi, 110067, India.
FAU - Ahamad, Naseem
AU  - Ahamad N
AD  - Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru 
      University, New Delhi, 110067, India.
FAU - Rath, Pramod C
AU  - Rath PC
AD  - Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru 
      University, New Delhi, 110067, India. pcrath@mail.jnu.ac.in.
LA  - eng
GR  - BT/INF/22/SP45382/2022/Department of Biotechnology, Ministry of Science and 
      Technology, India/
PT  - Journal Article
DEP - 20240109
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Interferon Regulatory Factors)
RN  - 0 (Irf1 protein, mouse)
RN  - 0 (Irf2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Bone Marrow
MH  - Bone Marrow Cells
MH  - Cytoplasm
MH  - *Interferon Regulatory Factors/genetics
MH  - *Mesenchymal Stem Cells
OTO - NOTNLM
OT  - Bone marrow cells
OT  - Cellular localization
OT  - IRF-1 IRF-2 expression
OT  - Mesenchymal stem cells
EDAT- 2024/01/09 13:43
MHDA- 2024/01/10 06:42
CRDT- 2024/01/09 11:16
PHST- 2023/08/17 00:00 [received]
PHST- 2023/11/27 00:00 [accepted]
PHST- 2024/01/10 06:42 [medline]
PHST- 2024/01/09 13:43 [pubmed]
PHST- 2024/01/09 11:16 [entrez]
AID - 10.1007/s11033-023-09025-9 [pii]
AID - 10.1007/s11033-023-09025-9 [doi]
PST - epublish
SO  - Mol Biol Rep. 2024 Jan 9;51(1):97. doi: 10.1007/s11033-023-09025-9.