PMID- 3819434
OWN - NLM
STAT- MEDLINE
DCOM- 19870409
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 97
IP  - 1
DP  - 1987 Feb 26
TI  - Cell cycle kinetics model of LPS-stimulated spleen cells correlates switch region 
      rearrangements with S phase.
PG  - 111-7
AB  - The cell cycle kinetics of lipopolysaccharide (LPS)-stimulated spleen cells were 
      measured by acridine orange (AO) staining and flow cytometry. We have devised a 
      computer model to predict the proportions of cells in each cell cycle phase using 
      iteratively varied parameters. The optimum fit between the predicted and observed 
      proportions of cells in various phases of the cell cycle was determined using the 
      minimum sigma chi 2. The model then correlates the variability of intermitotic 
      phase time with the proportion of genomic DNA available for immunoglobulin (Ig) 
      switch region (S mu) rearrangement. This analysis predicts that rearrangements at 
      S mu are cell cycle-dependent events which occur during the first S phase after 
      LPS activation. Molecular analysis of this system confirms these predictions.
FAU - Kenter, A L
AU  - Kenter AL
FAU - Watson, J V
AU  - Watson JV
LA  - eng
GR  - R01 AI052400/AI/NIAID NIH HHS/United States
GR  - R21 AI106328/AI/NIAID NIH HHS/United States
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - *Cell Cycle
MH  - Cells, Cultured
MH  - Interphase
MH  - Kinetics
MH  - Lipopolysaccharides/immunology
MH  - Lymphocytes/cytology/*immunology
MH  - Mathematics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Biological
MH  - Spleen/immunology
EDAT- 1987/02/26 00:00
MHDA- 1987/02/26 00:01
CRDT- 1987/02/26 00:00
PHST- 1987/02/26 00:00 [pubmed]
PHST- 1987/02/26 00:01 [medline]
PHST- 1987/02/26 00:00 [entrez]
AID - 0022-1759(87)90112-8 [pii]
AID - 10.1016/0022-1759(87)90112-8 [doi]
PST - ppublish
SO  - J Immunol Methods. 1987 Feb 26;97(1):111-7. doi: 10.1016/0022-1759(87)90112-8.